{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:42:22Z","timestamp":1760240542205,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Influenza A viruses leading to infectious respiratory diseases cause seasonal epidemics and sometimes periodic global pandemics. Viral polymerase is an attractive target in inhibiting viral replication, and 4\u2032-ethynyladenosine, which has been reported as a highly potent anti-human immunodeficiency virus (HIV) nucleoside derivative, can work as an anti-influenza agent. Herein, we designed and synthesized a 4\u2032-ethynyl-2\u2032-deoxyadenosine 5\u2032-monophosphate analog called EdAP (5). EdAP exhibited potent inhibition against influenza virus multiplication in Madin\u2013Darby canine kidney (MDCK) cells transfected with human \u03b12-6-sialyltransferase (SIAT1) cDNA and did not show any toxicity toward the cells. Surprisingly, this DNA-type nucleic acid analog (5) inhibited the multiplication of influenza A virus, although influenza virus is an RNA virus that does not generate DNA.<\/jats:p>","DOI":"10.3390\/molecules24142603","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T11:25:12Z","timestamp":1563362712000},"page":"2603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Design, Synthesis, and Biological Evaluation of EdAP, a 4\u2032-Ethynyl-2\u2032-Deoxyadenosine 5\u2032-Monophosphate Analog, as a Potent Influenza a Inhibitor"],"prefix":"10.3390","volume":"24","author":[{"given":"Toshifumi","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nongluk","family":"Sriwilaijaroen","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand"},{"name":"Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Sakuraba","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ei","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Kamisuki","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Ohrui","sequence":"additional","affiliation":[{"name":"Yokohama University of Pharmacy, Matano-cho 601, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,17]]},"reference":[{"key":"ref_1","unstructured":"(2019). 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