{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T23:59:06Z","timestamp":1782518346588,"version":"3.54.5"},"reference-count":24,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["107-2113-M-001-001"],"award-info":[{"award-number":["107-2113-M-001-001"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A group of prenyltransferases catalyze chain elongation of farnesyl diphosphate (FPP) to designated lengths via consecutive condensation reactions with specific numbers of isopentenyl diphosphate (IPP). cis-Prenyltransferases, which catalyze cis-double bond formation during IPP condensation, usually synthesize long-chain products as lipid carriers to mediate peptidoglycan biosynthesis in prokaryotes and protein glycosylation in eukaryotes. Unlike only one or two cis-prenyltransferases in bacteria, yeast, and animals, plants have several cis-prenyltransferases and their functions are less understood. As reported here, a cis-prenyltransferase from Lilium longiflorum anther, named LLA66, was expressed in Saccharomyces cerevisiae and characterized to produce C40\/C45 products without the capability to restore the growth defect from Rer2-deletion, although it was phylogenetically categorized as a long-chain enzyme. Our studies suggest that evolutional mutations may occur in the plant cis-prenyltransferase to convert it into a shorter-chain enzyme.<\/jats:p>","DOI":"10.3390\/molecules24152728","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T03:06:58Z","timestamp":1564369618000},"page":"2728","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther"],"prefix":"10.3390","volume":"24","author":[{"given":"Jyun-Yu","family":"Yao","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Hsun","family":"Teng","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0032-1288","authenticated-orcid":false,"given":"Ming-Che","family":"Liu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Co-Shine","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Huang","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,26]]},"reference":[{"key":"ref_1","unstructured":"Porter, J.W., and Spurgeon, S.L. 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