{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:25:32Z","timestamp":1789730732625,"version":"4.0.1"},"reference-count":35,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["21151406"],"award-info":[{"award-number":["21151406"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Antofagasta","award":["NEXER; Project [ANT1756]"],"award-info":[{"award-number":["NEXER; Project [ANT1756]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Alkaloids derived from plants have shown great medicinal benefits, and are often reported for their use in cardiovascular disease management. Aristotelia chilensis (Molina) Stuntz (Maqui) has shown important medicinal properties in traditional useage. In this study, we evaluated the effect of the indole-alkaloid aristoteline (ARI), isolated from leaves of Maqui, on vascular reactivity of isolated aortic rings from normotensive rats. ARI induced relaxation (100%) in a concentration-dependent manner in intact or denuded-endothelium aortic rings pre-contracted with phenylephrine (PE; 1 \u03bcM). However, a specific soluble guanylyl cyclase inhibitor (ODQ; 1 \u03bcM) significantly reduced the relaxation to ARI in aortic rings pre-contracted with PE. In the presence of ARI, the contraction induced by KCl or PE was significantly (p &lt; 0.05) decreased. Interestingly, the potassium channel blockade with 10 \u03bcM BaCl2 (Kir), 10 \u03bcM glibenclamide (KATP), 1 mM tetraethylammonium (TEA; KCa1.1), or 1 mM 4-aminopyridine (4-AP; Kv) significantly (p &lt; 0.05) reduced the ARI-induced relaxation. ARI significantly (p &lt; 0.05) reduced the contractile response to agonist of CaV1.2 channels (Bay K8644; 10 nM), likely reducing the influx of extracellular calcium through plasma membrane. The mechanisms associated with this process suggest an activation of the potassium channels, a calcium-induced antagonism and endothelium independent vasodilation that possibly involves the nitric oxide-independent soluble guanylate cyclase pathway.<\/jats:p>","DOI":"10.3390\/molecules24152748","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T11:20:18Z","timestamp":1564399218000},"page":"2748","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8189-0954","authenticated-orcid":false,"given":"Fernando","family":"Romero","sequence":"first","affiliation":[{"name":"Vicerrector\u00eda de Investigaci\u00f3n y Postgrado, Programa de Doctorado en Ciencias M\u00e9dicas, Universidad de la Frontera, Temuco 4780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7997-9295","authenticated-orcid":false,"given":"Javier","family":"Palacios","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Aplicada, Departamento de Qu\u00edmica y Farmacia, Facultad Ciencias de la Salud, Universidad Arturo Prat, Iquique 1110939, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0142-2446","authenticated-orcid":false,"given":"Ignacio","family":"Jofr\u00e9","sequence":"additional","affiliation":[{"name":"Laboratorio de Neurociencias y Biolog\u00eda de P\u00e9ptidos, Centro de Excelencia en Biotecnolog\u00eda de La Reproducci\u00f3n, Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4668-4984","authenticated-orcid":false,"given":"Cristian","family":"Paz","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales y Descubrimiento de F\u00e1rmacos, Departamento de Ciencias b\u00e1sicas, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7092-9382","authenticated-orcid":false,"given":"Chukwuemeka R.","family":"Nwokocha","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences Physiology Section, Faculty of Medical Sciences, The University of the West Indies, Mona, Kingston 7, KGN, Jamaica (W.I.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-8582","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Paredes","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica Biol\u00f3gica, Instituto Antofagasta, Universidad de Antofagasta, Antofagasta 1270300, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5922-8892","authenticated-orcid":false,"given":"Fredi","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Experimental, Instituto Antofagasta, Universidad de Antofagasta, Antofagasta 1270300, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1007\/s11101-017-9533-1","article-title":"Phytochemistry and biological properties of Aristotelia chilensis a Chilean blackberry: a review","volume":"16","author":"Zuniga","year":"2017","journal-title":"Phytochem. 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