{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:24:09Z","timestamp":1788470649721,"version":"build-2803163510"},"reference-count":28,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T00:00:00Z","timestamp":1566345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SHANGHAI SAILING PROGRAM","award":["17YF1413600"],"award-info":[{"award-number":["17YF1413600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Many studies have shown that Orthosiphon stamineus extract (OE) has antioxidant activity, and we previously reported that OE protects the intestine against injury from a high-fat diet. However, the molecular mechanism underlying this protective effect of OE was unclear. Here, OE was separated according to polarity and molecular weight, and the antioxidant activity of each component was compared. The components with the highest antioxidant activity were analyzed by HPLC, which confirmed that rosmarinic acid (RA) was the main effective constituent in OE. OE and RA were then tested in a mouse high-fat diet-induced intestinal injury model. The antioxidant indices and morphological characteristics of the mouse jejunum were measured, and activation of the nuclear factor E2-related factor 2 (Nrf2) pathway and apoptosis of jejunal epithelial cells were analyzed. Of all the constituents in OE, RA contributed the most. Both RA and OE activated the Nrf2 pathway and increased downstream antioxidant enzyme activity. RA and OE protected the mouse intestine against high-fat diet-induced oxidative stress by preventing intestinal epithelial cell apoptosis via both extracellular and intracellular pathways. Thus, RA, the main effective constituent in OE, inhibits intestinal epithelial apoptosis by regulating the Nrf2 pathway in mice.<\/jats:p>","DOI":"10.3390\/molecules24173027","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T11:19:06Z","timestamp":1566386346000},"page":"3027","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Rosmarinic Acid, the Main Effective Constituent of Orthosiphon stamineus, Inhibits Intestinal Epithelial Apoptosis Via Regulation of the Nrf2 Pathway in Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4413-8637","authenticated-orcid":false,"given":"Xuan","family":"Cai","sequence":"first","affiliation":[{"name":"Shanghai Shenfeng Animal Husbandry and Veterinary Science Technology Co., Ltd., Shanghai 201106, China"},{"name":"Institute of Animal Husbandry &amp; Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai 201106, China"},{"name":"Shanghai Engineering Research Center of Breeding Pig, Shanghai 201106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Biology Department, College of Life and Environment Science, Shanghai Normal University,100 Guilin Road, Shanghai 200234, China"},{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China"},{"name":"Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry &amp; Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai 201106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Xia","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry &amp; Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai 201106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Biology Department, College of Life and Environment Science, Shanghai Normal University,100 Guilin Road, Shanghai 200234, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqin","family":"Xue","sequence":"additional","affiliation":[{"name":"Shanghai Shenfeng Animal Husbandry and Veterinary Science Technology Co., Ltd., Shanghai 201106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Lu","sequence":"additional","affiliation":[{"name":"Shanghai Shenfeng Animal Husbandry and Veterinary Science Technology Co., Ltd., Shanghai 201106, China"},{"name":"Institute of Animal Husbandry &amp; Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai 201106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.3945\/jn.108.104125","article-title":"Western-Style Diets Induce Oxidative Stress and Dysregulate Immune Responses in the Colon in a Mouse Model of Sporadic Colon Cancer","volume":"139","author":"Erdelyi","year":"2009","journal-title":"J. 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