{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:22:03Z","timestamp":1785813723508,"version":"3.56.0"},"reference-count":38,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Jiangxi Provincial Education Department","award":["YC2018-B073"],"award-info":[{"award-number":["YC2018-B073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Gouty arthritis (GA) is commonly caused by deposition of monosodium urate (MSU) crystals within the joint capsule, bursa, cartilage, bone, or other periarticular tissues after chronic hyperuricemia. Clinically, GA is characterized by acute episodes of joint inflammation, which is most frequently encountered in the major joints, and also has a significant impact on quality of life. Pulchinenoside b4(P-b4) has a wide range of biological activities, including antitumor, anti-inflammatory, antiviral and immunomodulatory activities. Currently, the anti-GA activity and metabolomic profiles after being treated by P-b4 have not been reported. In this paper, for the first time, we have performed a non-targeted metabolomics analysis of serum obtained from an MSU crystal-induced GA rat model intervened by P-b4, using ultra-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. In this study, the main pharmacodynamics of different dosing methods and dosages of P-b4 was firstly investigated. Results have shown that P-b4 possesses high anti-inflammatory activity. These results demonstrated changes in serum metabolites with 32 potential biomarkers. Arachidonic acid, sphingolipid, and glycerophospholipid metabolism are considered to be the most relevant metabolic pathway with P-b4 treatment effect in this study. Moreover, the changes of metabolites and the self-extinction of model effects within 24 h reveals important information for GA diagnostic criteria: The regression of clinical symptoms or the decline of some biochemical indicators cannot be regarded as the end point of GA treatment. Furthermore, our research group plans to conduct further metabolomics research on the clinical course of GA.<\/jats:p>","DOI":"10.3390\/molecules24173161","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T10:31:17Z","timestamp":1567161077000},"page":"3161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["LC-MS Analysis of Serum for the Metabolomic Investigation of the Effects of Pulchinenoside b4 Administration in Monosodium Urate Crystal-Induced Gouty Arthritis Rat Model"],"prefix":"10.3390","volume":"24","author":[{"given":"Shang","family":"Lyu","sequence":"first","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruowen","family":"Ding","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Liu","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"OuYang","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"},{"name":"State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Feng","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"},{"name":"State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Rao","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilin","family":"Yang","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/nrrheum.2015.91","article-title":"Global epidemiology of gout: Prevalence, incidence and risk factors","volume":"11","author":"Kuo","year":"2015","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1056\/NEJMcp1001124","article-title":"Gout","volume":"364","author":"Neogi","year":"2011","journal-title":"N Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S0140-6736(09)60883-7","article-title":"Gout","volume":"375","author":"Richette","year":"2010","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1016\/S0140-6736(16)00346-9","article-title":"Gout","volume":"388","author":"Dalbeth","year":"2016","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jbspin.2012.09.017","article-title":"Polyarticular sonographic assessment of gout: A hospital-based cross-sectional study","volume":"80","author":"Roddy","year":"2013","journal-title":"Joint Bone Spine"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e3914","DOI":"10.1002\/bmc.3914","article-title":"Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion in the study of anemoside B4, a novel antiviral agent candidate, in rats","volume":"31","author":"He","year":"2017","journal-title":"Biomed. Chromatogr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3109\/08923970902814129","article-title":"Chinese herbal medicinal ingredients inhibit secretion of IL-6, IL-8, E-selectin and TXB(2) in LPS-induced rat intestinal microvascular endothelial cells","volume":"31","author":"Hu","year":"2009","journal-title":"Immunopharm. Immunot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3109\/1547691X.2015.1017624","article-title":"Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells","volume":"13","author":"Hu","year":"2016","journal-title":"J. Immunot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/13880209.2014.907323","article-title":"Cytotoxicity of the compounds isolated from Pulsatilla chinensis saponins and apoptosis induced by 23-hydroxybetulinic acid","volume":"53","author":"Liu","year":"2015","journal-title":"Pharm. Bio."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nrrheum.2017.155","article-title":"Inflammation in gout: Mechanisms and therapeutic targets","volume":"13","author":"So","year":"2017","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nri.2016.58","article-title":"Inflammasomes: Mechanism of assembly, regulation and signalling","volume":"16","author":"Broz","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.taap.2018.05.010","article-title":"Inhibition of COX-2\/mPGES-1 and 5-LOX in macrophages by leonurine ameliorates monosodium urate crystal-induced inflammation","volume":"351","author":"Liu","year":"2018","journal-title":"Toxicolo Appl. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Thottam, G.E., Krasnokutsky, S., and Pillinger, M.H. (2017). Gout and Metabolic Syndrome: A Tangled Web. Curr. Rheumatol. Rep., 19.","DOI":"10.1007\/s11926-017-0688-y"},{"key":"ref_14","first-page":"443","article-title":"Analytical strategies in metabonomics","volume":"1","author":"Eva","year":"2007","journal-title":"J. Proteome Res."},{"key":"ref_15","first-page":"4182","article-title":"Application of metabolomics and related technologies in the R&D field of traditional Chinese medicine","volume":"43","author":"Lyu","year":"2018","journal-title":"China J. Chin. Mater. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11083","DOI":"10.1021\/acs.analchem.7b03160","article-title":"AntDAS: Automatic Data Analysis Strategy for UPLC-QTOF-Based Nontargeted Metabolic Profiling Analysis","volume":"89","author":"Fu","year":"2017","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, X.Y., Li, J.M., Xie, B., Wu, B., Lei, S., Yao, Y., He, M.Z., Ouyang, H., Feng, Y.L., and Xu, W. (2018). Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.00282"},{"key":"ref_18","first-page":"S90","article-title":"Alteration of the microRNA expression profile in familial Mediterranean fever patients","volume":"35","author":"Karadag","year":"2017","journal-title":"Clin. Exp. Rheumatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"W486","DOI":"10.1093\/nar\/gky310","article-title":"MetaboAnalyst 4.0: Towards more transparent and integrative metabolomics analysis","volume":"46","author":"Chong","year":"2018","journal-title":"Nucl. Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00296-017-3891-2","article-title":"An investigation of the independent risk factors that differentiate gout from pseudogout in patients with crystal-induced acute arthritis: A cross-sectional study","volume":"38","author":"Muangchan","year":"2017","journal-title":"Rheumatol. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1007\/s00330-016-4237-2","article-title":"Gouty arthritis: The diagnostic and therapeutic impact of dual-energy CT","volume":"26","author":"Finkenstaedt","year":"2016","journal-title":"Eur. Radiol."},{"key":"ref_22","first-page":"687","article-title":"BET 1: Prednisolone for the treatment of acute gouty arthritis","volume":"34","year":"2017","journal-title":"Emerg. Med. J."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Van Walsem, A., Pandhi, S., Nixon, R.M., Guyot, P., Karabis, A., and Moore, R.A. (2015). Relative benefit-risk comparing diclofenac to other traditional non-steroidal anti-inflammatory drugs and cyclooxygenase-2 inhibitors in patients with osteoarthritis or rheumatoid arthritis: A network meta-analysis. Arthritis Res. Ther., 17.","DOI":"10.1186\/s13075-015-0554-0"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3109\/14397595.2013.874732","article-title":"Colchicine-responsive protracted gouty arthritis with systemic inflammatory reactions","volume":"24","author":"Nonaka","year":"2014","journal-title":"Mod. Rheumatol."},{"key":"ref_25","first-page":"25","article-title":"The CD25+\/CD4+ T cell ratio and levels of CII, CIX and CXI antibodies in serum may serve as biomarkers of pristane-induced arthritis in rats and Rheumatoid Arthritis in humans","volume":"217","author":"Chen","year":"2019","journal-title":"Comp. Biochem. Phys. C"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Otsubo, H., Tsuneyoshi, Y., Nakamura, T., Matsuda, T., Komiya, S., and Matsuyama, T. (2018). Serum-soluble folate receptor \u03b2 as a biomarker for the activity of rheumatoid arthritis synovitis and the response to anti-TNF agents. Clin. Rheumatol.","DOI":"10.1007\/s10067-018-4202-3"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1007\/s10067-015-2922-1","article-title":"Serum microRNAs as Potential Biomarkers of Juvenile Idiopathic Arthritis","volume":"34","author":"Kamiya","year":"2015","journal-title":"Clin. Rheumatol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Daniela, C., Kun, L., Punit, S., Ihor, B., Eleftherios, P.D., and Vinod, C. (2015). Quantitative tandem mass-spectrometry of skin tissue reveals putative psoriatic arthritis biomarkers. Clin. Proteom., 12.","DOI":"10.1186\/1559-0275-12-1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jbspin.2018.04.008","article-title":"Comparison of several biomarkers (MMP-2, MMP-9, the MMP-9 inhibitor TIMP-1, CTX-II, calprotectin, and COMP) in the synovial fluid and serum of patients with and without septic arthritis","volume":"86","author":"Couderc","year":"2019","journal-title":"Joint Bone Spine"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.jiac.2018.10.015","article-title":"Early diagnosis of septic arthritis using synovial fluid presepsin: A preliminary study","volume":"25","author":"Imagama","year":"2019","journal-title":"J. Infect. Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, W., Yang, G.J., Zhang, J., Chen, C., Jia, Z.Y., Li, J., and Xu, W.D. (2016). Plasma, urine and ligament tissue metabolite profiling reveals potential biomarkers of ankylosing spondylitis using NMR-based metabolic profiles. Arthritis Res. Ther., 18.","DOI":"10.1186\/s13075-016-1139-2"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Stefano, A., Dario, B., Barbara, T., Laura, B., Luca, P., Maria, R., Domenico, B., Anna, L., Giusy, P., and Francesco, F. (2019). Differential synovial tissue biomarkers among psoriatic arthritis and rheumatoid factor\/anti-citrulline antibody-negative rheumatoid arthritis. Arthritis Res. Ther., 21.","DOI":"10.1186\/s13075-019-1898-7"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1021\/acs.accounts.5b00226","article-title":"Diverse ways of perturbing the human arachidonic acid metabolic network to control inflammation","volume":"48","author":"Meng","year":"2015","journal-title":"Accounts Chem. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s11306-014-0669-3","article-title":"Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism","volume":"11","author":"Jung","year":"2014","journal-title":"Metabolomics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1021\/pr070183p","article-title":"Within-day reproducibility of an HPLC-MS-based method for metabonomic analysis: Application to human urine","volume":"6","author":"Gika","year":"2007","journal-title":"J. Proteome Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zou, L., Zhang, Y., Li, W., Zhang, J.M., Wang, D., Fu, J., and Wang, P. (2017). Comparison of Chemical Profiles, Anti-Inflammatory Activity, and UPLC-Q-TOF\/MS-Based Metabolomics in Endotoxic Fever Rats between Synthetic Borneol and Natural Borneol. Molecules, 22.","DOI":"10.3390\/molecules22091446"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zheng, T.T., Su, S.L., Dai, X.X., Zhang, L.W., Duan, J.M., and OuYang, Z. (2018). Metabolomic Analysis of Biochemical Changes in the Serum and Urine of Freund\u2019s Adjuvant-Induced Arthritis in Rats after Treatment with Silkworm Excrement. Molecules, 23.","DOI":"10.3390\/molecules23061490"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/nmeth.3484","article-title":"Phenolyzer: Phenotype-based prioritization of candidate genes for human diseases","volume":"12","author":"Hang","year":"2015","journal-title":"Nat. Methods"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/17\/3161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:15:17Z","timestamp":1760188517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/17\/3161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,30]]},"references-count":38,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["molecules24173161"],"URL":"https:\/\/doi.org\/10.3390\/molecules24173161","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,30]]}}}