{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:45:27Z","timestamp":1760240727261,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/06034-2"],"award-info":[{"award-number":["2016\/06034-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["301971\/2017-0","404769\/2018-7"],"award-info":[{"award-number":["301971\/2017-0","404769\/2018-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["Global Challenges Research Funder - A Global Network for Neglected Tropical Diseases (MR\/P027989\/1)"],"award-info":[{"award-number":["Global Challenges Research Funder - A Global Network for Neglected Tropical Diseases (MR\/P027989\/1)"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 0641"],"award-info":[{"award-number":["PIP 0641"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Trypanosoma cruzi, the etiological agent of Chagas disease, is dependent on proline for a variety of processes, such as energy metabolism, host cell invasion, differentiation, and resistance to osmotic, metabolic, and oxidative stress. On this basis, we investigated a possible relationship between prolinemia and severity of T. cruzi infection in chronic patients, as reported here. The study population consisted of 112 subjects, separated into 83 chronically T. cruzi-infected patients and 29 age-matched healthy volunteers (control) of both sexes, recruited at the Chagas Disease Service from the Department of Cardiology, Hospital Provincial del Centenario de Rosario (Rosario, Argentina). Chagasic patients were separated into three groups: chronic asymptomatic, mild\/moderate, and severe chronic chagasic cardiomyopathy (CCC) subjects. We observed a significant decrease of 11.7% in prolinemia in chagasic patients when compared to controls. Further analysis within the three groups of chagasic patients also revealed a statistically significant decrease of prolinemia in severe CCC patients compared to controls, showing a relative difference of 13.6% in proline concentrations. These data point to the possibility that collagen\u2014which participates in the healing process of cardiac tissue\u2014and proline metabolism in the myocardium could constitute new factors affecting the evolution of Chagas disease.<\/jats:p>","DOI":"10.3390\/molecules24173167","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T10:31:17Z","timestamp":1567161077000},"page":"3167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diminished Prolinemia in Chronic Chagasic Patients: A New Clue for Disease Pathology?"],"prefix":"10.3390","volume":"24","author":[{"given":"Sandra Carla","family":"Rocha","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry of Tryps\u2014LaBTryps, Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, Av. Prof. Lineu Prestes 1374, Sao Paulo 05508000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Rosa","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER-CONICET UNR), Suipacha 590, Rosario 2000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Beloscar","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER-CONICET UNR), Suipacha 590, Rosario 2000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2526-7281","authenticated-orcid":false,"given":"Oscar","family":"Bottasso","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER-CONICET UNR), Suipacha 590, Rosario 2000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4528-4732","authenticated-orcid":false,"given":"Ariel Mariano","family":"Silber","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry of Tryps\u2014LaBTryps, Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, Av. Prof. Lineu Prestes 1374, Sao Paulo 05508000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"ref_1","unstructured":"(2018, March 20). World Health Organization Chagas Disease (American Trypanosomiasis) Home Page. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs340\/en\/."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi","year":"2010","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.molbiopara.2009.07.006","article-title":"Glucose uptake in the mammalian stages of Trypanosoma cruzi","volume":"168","author":"Silber","year":"2009","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Magdaleno, A., Ahn, I.Y., Paes, L.S., and Silber, A.M. (2009). 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Anal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/BF00018060","article-title":"Rapid determination of free proline for water-stress studies","volume":"39","author":"Bates","year":"1973","journal-title":"Plant Soil"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/17\/3167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:15:32Z","timestamp":1760188532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/17\/3167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,30]]},"references-count":23,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["molecules24173167"],"URL":"https:\/\/doi.org\/10.3390\/molecules24173167","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2019,8,30]]}}}