{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:47:23Z","timestamp":1781279243806,"version":"3.54.1"},"reference-count":48,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T00:00:00Z","timestamp":1567728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Green tea is a popular beverage that is rich in polyphenolic compounds such as catechins. Its major content, (-)-epigallocatechin-3-gallate, has been shown to have beneficial effects on several diseases including cancer, metabolic syndrome, cardiovascular diseases, and neurodegenerative diseases. The aim of this study was to assess the anti-obesity effects and the underlying molecular mechanisms of green tea extract (GTE) using zebrafish larva and adult obesity models. We administered 100 \u03bcg\/mL GTE to zebrafish larvae and performed a short-term obesogenic test. GTE significantly decreased the visceral adipose tissue volume induced by a high-fat diet. Oral administration (250 \u00b5g\/g body weight\/day) of GTE to adult diet-induced obese zebrafish also significantly reduced their visceral adipose tissue volume, with a reduction of plasma triglyceride and total cholesterol levels. To investigate the molecular mechanism underlying the GTE effects, we conducted RNA sequencing using liver tissues of adult zebrafish and found that GTE may ameliorate the obese phenotypes via the activation of Wnt\/\u03b2-catenin and adenosine monophosphate-activated protein kinase (AMPK) pathway signaling. In addition, the comparative transcriptome analysis revealed that zebrafish and mammals may share a common molecular response to GTE. Our findings suggest that daily consumption of green tea may be beneficial for the prevention and treatment of obesity.<\/jats:p>","DOI":"10.3390\/molecules24183256","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T03:14:41Z","timestamp":1567998881000},"page":"3256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["RNA-seq Based Transcriptome Analysis of the Anti-Obesity Effect of Green Tea Extract Using Zebrafish Obesity Models"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-795X","authenticated-orcid":false,"given":"Liqing","family":"Zang","sequence":"first","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan"},{"name":"Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-8262","authenticated-orcid":false,"given":"Yasuhito","family":"Shimada","sequence":"additional","affiliation":[{"name":"Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan"},{"name":"Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan"},{"name":"Department of Bioinformatics, Mie University Life Science Research Centre, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-6428","authenticated-orcid":false,"given":"Hiroko","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan"},{"name":"Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngil","family":"Kim","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djong-Chi","family":"Chu","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lekh Raj","family":"Juneja","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Kuroyanagi","sequence":"additional","affiliation":[{"name":"UMOU Science Lab, Mastusaka, Mie 515-0303, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-4013","authenticated-orcid":false,"given":"Norihiro","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan"},{"name":"Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.foodchem.2005.03.015","article-title":"Extraction of active ingredients from green tea (Camellia sinensis): Extraction efficiency of major catechins and caffeine","volume":"96","author":"Skerget","year":"2006","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1039\/b102541b","article-title":"Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection","volume":"126","author":"Sano","year":"2001","journal-title":"Analyst"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1111\/bph.12009","article-title":"Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea","volume":"168","author":"Steinmann","year":"2013","journal-title":"Br. 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