{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:47:05Z","timestamp":1791863225836,"version":"4.3.4"},"reference-count":86,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BlueSky Research Grant received from the University of Pavia","award":["BSR17"],"award-info":[{"award-number":["BSR17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>An LC-MS\/MS method for the identification and quantification of antidepressants and antipsychotics was developed on dried blood spots (DBSs). Moreover, analyte stability on DBSs within a 3-month period was monitored. Aliquots of 85 \u00b5L of blood from autopsy cases were pipetted onto DBS cards, which were dried and stored at room temperature. DBSs were analyzed in triplicate immediately, within the following 3 weeks, and after 3 months. For each analysis, a whole blood stain was extracted in phosphate buffer and purified using Solid Phase Extraction (SPE) cartridges in order to avoid matrix effects and injected in the LC-MS\/MS system. Thirty-nine molecules were screened. Limits of detection (LODs) ranged between 0.1 and 3.2 ng\/mL (g) and 0.1 and 5.2 ng\/mL (g) for antidepressants and antipsychotics, respectively. Limits of quantification (LOQs) varied from 5 to 10.0 ng\/mL for both. Sixteen cases among the 60 analyzed resulted positive for 17 different analytes; for 14 of these the method was fully validated. A general good agreement between the concentrations on DBSs and those measured in conventional blood samples (collected concurrently and stored at \u221220 \u00b0C) was observed. The degradation\/enhancement percentage for most of the substances was lower than 20% within the 3-month period. Our results, obtained from real post-mortem cases, suggest that DBSs can be used for routine sample storage.<\/jats:p>","DOI":"10.3390\/molecules24203636","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T11:25:57Z","timestamp":1570620357000},"page":"3636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Determination of Antidepressants and Antipsychotics in Dried Blood Spots (DBSs) Collected from Post-Mortem Samples and Evaluation of the Stability over a Three-Month Period"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-9366","authenticated-orcid":false,"given":"Matteo","family":"Moretti","sequence":"first","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Freni","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Valentini","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Vignali","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Groppi","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Damiana","family":"Vison\u00e0","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Marco Maria","family":"Osculati","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Morini","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.addbeh.2018.08.027","article-title":"A systematic review into the incidence, severity and duration of antidepressant withdrawal effects: Are guidelines evidence-based?","volume":"97","author":"Davies","year":"2018","journal-title":"Addict. Behav."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jad.2019.05.025","article-title":"Antidepressant pharmacogenetics in children and young adults: A systematic review","volume":"254","author":"Maruf","year":"2019","journal-title":"J. Affect. Disord."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/S0140-6736(17)32802-7","article-title":"Comparative efficacy and acceptability of 21 antidepressant drugs for the acute treatment of adults with major depressive disorder: A systematic review and network meta-analysis","volume":"391","author":"Cipriani","year":"2018","journal-title":"Lancet"},{"key":"ref_4","first-page":"123","article-title":"Mechanism of action of antidepressants","volume":"36","author":"Artigas","year":"2002","journal-title":"Psychopharmacol. Bull."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"438","DOI":"10.5694\/mja14.01116","article-title":"A prospective cohort study of trends in self-poisoning, Newcastle, Australia, 1987-2012: Plus ca change, plus c\u2019est la meme chose","volume":"202","author":"Buckley","year":"2015","journal-title":"Med. J. Aust."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/bcpt.12541","article-title":"Antidepressant or Antipsychotic Overdose in the Intensive Care Unit\u2014Identification of Patients at Risk","volume":"119","author":"Borg","year":"2016","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4068\/cmj.2018.54.2.101","article-title":"Addressing the Side Effects of Contemporary Antidepressant Drugs: A Comprehensive Review","volume":"54","author":"Wang","year":"2018","journal-title":"Chonnam. Med. J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Santarsieri, D., and Schwartz, T.L. (2015). Antidepressant efficacy and side-effect burden: A quick guide for clinicians. Drugs Context, 4.","DOI":"10.7573\/dic.212290"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/bcp.12927","article-title":"The antipsychotic story: Changes in prescriptions and overdose without better safety","volume":"82","author":"Berling","year":"2016","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_10","unstructured":"Chokhawala, K., and Stevens, L. (2019, April 22). Antipsychotic Medications, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK519503\/."},{"key":"ref_11","first-page":"1163","article-title":"Clinical pharmacology of atypical antipsychotics: An update","volume":"13","author":"Mauri","year":"2014","journal-title":"EXCLI J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/s11481-007-9091-1","article-title":"Cocaine mediated alteration in thitght juncion protein expression and modulation of CCL2\/CCR2 axis across the blood brain barrier: Implications for HIV dementia","volume":"3","author":"Dhillon","year":"2007","journal-title":"J. Neuroimmune. Pharm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2788.2009.01232.x","article-title":"Pharmacotherapy for aggressive behaviours in persons with intellectual disabilities: Treatment or mistreatment?","volume":"54","author":"Tsiouris","year":"2010","journal-title":"J. Intellect. Disabil. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/acps.12160","article-title":"Antidepressants and the risk of suicide in young persons\u2014Prescription trends and toxicological analyses","volume":"129","author":"Isacsson","year":"2014","journal-title":"Acta Psychiatr. Scand."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"324","DOI":"10.4103\/0019-5545.58304","article-title":"The story of antipsychotics: Past and present","volume":"51","author":"Ramachandraiah","year":"2009","journal-title":"Indian J. Psychiatry"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.pedhc.2011.10.009","article-title":"Antipsychotic Medication Prescribing Trends in Children and Adolescents","volume":"26","author":"Harrison","year":"2012","journal-title":"J. Pediatr. Health Care"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1093\/jat\/bky030","article-title":"Comparative Study of Postmortem Concentrations of Antidepressants in Several Different Matrices","volume":"42","author":"Oiestad","year":"2018","journal-title":"J. Anal. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00414-007-0178-2","article-title":"Frequency of different anti-depressants associated with suicides and drug deaths","volume":"122","author":"Drasch","year":"2008","journal-title":"Int. J. Legal. Med."},{"key":"ref_19","unstructured":"Goodman, L.S., Brunton, L.L., Chabner, B., and Knollmann, B.C. (2011). Goodman & Gilman\u2019s Pharmacological Basis of Therapeutics, McGraw-Hill."},{"key":"ref_20","unstructured":"Levine, B., and American Association for Clinical Chemistry (2013). Principles of Forensic Toxicology, AACC Press. [4th ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.forsciint.2016.05.002","article-title":"Antipsychotics\u2014Postmortem fatal and non-fatal reference concentrations","volume":"266","author":"Soderberg","year":"2016","journal-title":"Forensic Sci. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1111\/j.1528-1167.2009.02204.x","article-title":"A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC-tandem mass spectrometry","volume":"50","author":"Malvagia","year":"2009","journal-title":"Epilepsia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1097\/FTD.0b013e31819e91ce","article-title":"Dried blood spot methods in therapeutic drug monitoring: Methods, assays, and pitfalls","volume":"31","author":"Edelbroek","year":"2009","journal-title":"Ther. Drug Monit."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1353\/dem.2007.0038","article-title":"What a drop can do: Dried blood spots as a minimally invasive method for integrating biomarkers into population-based research","volume":"44","author":"McDade","year":"2007","journal-title":"Demography"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/bmc.1367","article-title":"Dried blood spot sampling in combination with LC-MS\/MS for quantitative analysis of small molecules","volume":"24","author":"Li","year":"2010","journal-title":"Biomed. Chromatogr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1542\/peds.32.3.338","article-title":"A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants","volume":"32","author":"Guthrie","year":"1963","journal-title":"Pediatrics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.trac.2017.12.004","article-title":"Dried matrix spots and clinical elemental analysis. Current status, difficulties, and opportunities","volume":"99","author":"Resano","year":"2018","journal-title":"TrAC Trend. Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.4155\/bio.10.73","article-title":"Performance properties of filter paper devices for whole blood collection","volume":"2","author":"Mei","year":"2010","journal-title":"Bioanalysis"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.clinbiochem.2010.06.014","article-title":"The analysis of dried blood spot samples using liquid chromatography tandem mass spectrometry","volume":"44","author":"Keevil","year":"2011","journal-title":"Clin. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.pharep.2013.06.012","article-title":"Oral dosing in adult zebrafish: Proof-of-concept using pharmacokinetics and pharmacological evaluation of carbamazepine","volume":"66","author":"Kulkarni","year":"2014","journal-title":"Pharmacol. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"230","DOI":"10.3109\/10408444.2011.650790","article-title":"Dried blood spots in toxicology: From the cradle to the grave?","volume":"42","author":"Stove","year":"2012","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.talanta.2014.10.041","article-title":"A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS\/MS","volume":"134","author":"Berm","year":"2015","journal-title":"Talanta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.clinbiochem.2016.12.008","article-title":"Comparison between dried blood spot and plasma sampling for therapeutic drug monitoring of antiepileptic drugs in children with epilepsy: A step towards home sampling","volume":"50","author":"Linder","year":"2017","journal-title":"Clin. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.euroneuro.2017.01.009","article-title":"Dried Blood Spot sampling in psychiatry: Perspectives for improving therapeutic drug monitoring","volume":"27","author":"Martial","year":"2017","journal-title":"Eur. Neuropsychopharmacol."},{"key":"ref_35","first-page":"288","article-title":"Advantages and Challenges of Dried Blood Spot Analysis by Mass Spectrometry Across the Total Testing Process","volume":"27","author":"Zakaria","year":"2016","journal-title":"EJIFCC"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.forsciint.2014.04.015","article-title":"Simplifying sample pretreatment: Application of dried blood spot (DBS) method to blood samples, including postmortem, for UHPLC-MS\/MS analysis of drugs of abuse","volume":"243","author":"Odoardi","year":"2014","journal-title":"Forensic Sci. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.chroma.2017.02.037","article-title":"Sensitive screening of abused drugs in dried blood samples using ultra-high-performance liquid chromatography-ion booster-quadrupole time-of-flight mass spectrometry","volume":"1491","author":"Chepyala","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jchromb.2011.11.035","article-title":"On-line liquid chromatography\/tandem mass spectrometry simultaneous determination of opiates, cocainics and amphetamines in dried blood spots","volume":"885\u2013886","author":"Saussereau","year":"2012","journal-title":"J. Chromatogr. B"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.jpba.2017.02.046","article-title":"Dried blood spots combined to an UPLC-MS\/MS method for the simultaneous determination of drugs of abuse in forensic toxicology","volume":"147","author":"Dias","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jpba.2016.05.011","article-title":"Identification and quantification of psychoactive drugs in whole blood using dried blood spot (DBS) by ultra-performance liquid chromatography tandem mass spectrometry","volume":"128","author":"Kyriakou","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1002\/dta.1505","article-title":"Rapid and simple LC-MS\/MS screening of 64 novel psychoactive substances using dried blood spots","volume":"6","author":"Ambach","year":"2014","journal-title":"Drug Test Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1007\/s00216-009-3412-6","article-title":"Use of the dried blood spot sampling process coupled with fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry: Application to fluoxetine, norfluoxetine, reboxetine, and paroxetine analysis","volume":"396","author":"Deglon","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1016\/j.jpba.2009.02.001","article-title":"On-line desorption of dried blood spot: A novel approach for the direct LC\/MS analysis of micro-whole blood samples","volume":"49","author":"Deglon","year":"2009","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1007\/s00216-014-7619-9","article-title":"Determination of venlafaxine and O-desmethylvenlafaxine in dried blood spots for TDM purposes, using LC-MS\/MS","volume":"406","author":"Berm","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1097\/FTD.0000000000000502","article-title":"Dried Blood Spot Analysis for Therapeutic Drug Monitoring of Antipsychotics: Drawbacks of Its Clinical Application","volume":"40","author":"Kloosterboer","year":"2018","journal-title":"Ther. Drug Monit."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1002\/dta.1698","article-title":"The use of dried blood spots for quantification of 15 antipsychotics and 7 metabolites with ultra-high performance liquid chromatography\u2014Tandem mass spectrometry","volume":"7","author":"Patteet","year":"2015","journal-title":"Drug Test Anal."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.4155\/bio.15.100","article-title":"Are capillary DBS applicable for therapeutic drug monitoring of common antipsychotics? A proof of concept","volume":"7","author":"Patteet","year":"2015","journal-title":"Bioanalysis"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1002\/dta.2399","article-title":"A liquid chromatography-tandem mass spectrometry method for the determination of cocaine and metabolites in blood and in dried blood spots collected from postmortem samples and evaluation of the stability over a 3-month period","volume":"10","author":"Moretti","year":"2018","journal-title":"Drug Test Anal."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1002\/dta.2653","article-title":"Determination of benzodiazepines in blood and in dried blood spots (DBS) collected from postmortem samples and evaluation of the stability over a three-month period","volume":"11","author":"Moretti","year":"2019","journal-title":"Drug Test Anal."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jchromb.2014.08.039","article-title":"Simple and sensitive screening and quantitative determination of 88 psychoactive drugs and their metabolites in blood through LC-MS\/MS: Application on postmortem samples","volume":"970","author":"Sempio","year":"2014","journal-title":"J. Chromatogr. B"},{"key":"ref_51","first-page":"5455","article-title":"Method Development in Forensic Toxicology","volume":"23","author":"Peters","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.forsciint.2011.02.022","article-title":"Assessment of the stability of 30 antipsychotic drugs in stored blood specimens","volume":"215","author":"Saar","year":"2012","journal-title":"Forensic Sci. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/ph3010019","article-title":"The Timing of Antidepressant Effects: A Comparison of Diverse Pharmacological and Somatic Treatments","volume":"3","author":"Baumann","year":"2010","journal-title":"Pharmaceuticals"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.forsciint.2013.03.021","article-title":"Stability of some atypical antipsychotics in human plasma, haemolysed whole blood, oral fluid, human serum and calf serum","volume":"229","author":"Fisher","year":"2013","journal-title":"Forensic Sci. Int."},{"key":"ref_55","first-page":"73","article-title":"Storage and Transport Stability of New Antidepressant and Antipsychotic Drugs","volume":"38","author":"Heller","year":"2005","journal-title":"Pharmacopsychiatry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.4155\/bio.11.132","article-title":"EBF recommendation on the validation of bioanalytical methods for dried blood spots","volume":"3","author":"Timmerman","year":"2011","journal-title":"Bioanalysis"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Zhang, J., Majumdar, T.K., Flarakos, J., and Tse, F.L.S. (2013). Best Practices in LC-MS Method Development and Validation for Dried Blood Spots. Handb. LC-MS Bioanal., 379\u2013389.","DOI":"10.1002\/9781118671276.ch30"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.forsciint.2017.08.028","article-title":"Death after 25C-NBOMe and 25H-NBOMe consumption","volume":"279","author":"Morini","year":"2017","journal-title":"Forensic Sci. Int."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/jat\/bky047","article-title":"Distribution of the Synthetic Cathinone alpha-Pyrrolidinohexiophenone in Biological Specimens","volume":"43","author":"Vignali","year":"2019","journal-title":"J. Anal. Toxicol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1097\/00007691-200408000-00019","article-title":"Assessment of storage and transport stability of new antidepressant and antipsychotic drugs for a nationwide TDM service","volume":"26","author":"Heller","year":"2004","journal-title":"Ther. Drug Monit."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1002\/jms.194","article-title":"Stable isotope dilution negative ion chemical ionization gas chromatography-mass spectrometry for the quantitative analysis of paroxetine in human plasma","volume":"36","author":"Leis","year":"2001","journal-title":"J. Mass. Spectrom."},{"key":"ref_62","first-page":"340","article-title":"Validation of a selective method for determination of paroxetine in human plasma by LC-MS\/MS","volume":"8","author":"Massaroti","year":"2005","journal-title":"J. Pharm. Pharm. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0378-4347(87)80160-3","article-title":"Determination of the new monoamine oxidase inhibitor moclobemide and three of its metabolites in biological fluids by high-performance liquid chromatography","volume":"420","author":"Geschke","year":"1987","journal-title":"J. Chromatogr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jchromb.2003.09.046","article-title":"Measurement of total mirtazapine and normirtazapine in plasma\/serum by liquid chromatography with fluorescence detection","volume":"798","author":"Morgan","year":"2003","journal-title":"J. Chromatogr. B"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0378-4347(93)80239-Z","article-title":"Determination of fluoxetine and norfluoxetine in human plasma by capillary gas chromatography with electron-capture detection","volume":"614","author":"Lantz","year":"1993","journal-title":"J. Chromatogr."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0378-4347(98)00460-5","article-title":"High-performance liquid chromatographic assay with fluorescence detection for the routine monitoring of the antidepressant mirtazapine and its demethyl metabolite in human plasma","volume":"721","author":"Maris","year":"1999","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"9294","DOI":"10.1021\/acs.analchem.5b02031","article-title":"Liquid chromatography, in combination with a quadrupole time-of-flight instrument, with sequential window acquisition of all theoretical fragment-ion spectra acquisition: Validated quantification of 39 antidepressants in whole blood as part of a simultaneous screening and quantification procedure","volume":"87","author":"Roemmelt","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0378-4347(01)00121-9","article-title":"Rapid determination of citalopram in human plasma by high-performance liquid chromatography","volume":"755","author":"Macek","year":"2001","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s00216-007-1267-2","article-title":"Stability of analytes in biosamples\u2014An important issue in clinical and forensic toxicology?","volume":"388","author":"Peters","year":"2007","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1093\/clinchem\/40.6.929","article-title":"Monitoring tricyclic antidepressant concentrations in serum by fluorescence polarization immunoassay compared with gas chromatography and HPLC","volume":"40","author":"Rao","year":"1994","journal-title":"Clin. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s00414-008-0287-6","article-title":"Determination of antidepressants in human postmortem blood, brain tissue, and hair using gas chromatography-mass spectrometry","volume":"123","author":"Wille","year":"2009","journal-title":"Int. J. Legal. Med."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0379-0738(92)90180-5","article-title":"Post-mortem toxico-kinetics of trazodone","volume":"56","author":"Martin","year":"1992","journal-title":"Forensic Sci. Int."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1520\/JFS2003433","article-title":"Stability of drugs in stored postmortem femoral blood and vitreous humor","volume":"49","author":"Holmgren","year":"2004","journal-title":"J. Forensic Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5807218","DOI":"10.1155\/2018\/5807218","article-title":"Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid Chromatography\/Tandem Mass Spectrometry (LC-MS\/MS)","volume":"2018","author":"Khelfi","year":"2018","journal-title":"Int. J. Anal. Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1007\/s00216-011-5187-9","article-title":"Ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte procedure for target screening and quantification in human blood plasma: Validation and application for 31 neuroleptics, 28 benzodiazepines, and Z-drugs","volume":"401","author":"Remane","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1093\/jat\/bku080","article-title":"Long-term storage of authentic postmortem forensic blood samples at \u221220 degrees C: Measured concentrations of benzodiazepines, central stimulants, opioids and certain medicinal drugs before and after storage for 16\u201318 years","volume":"38","author":"Karinen","year":"2014","journal-title":"J. Anal. Toxicol."},{"key":"ref_77","unstructured":"Lewis, R.J., Angier, M.K., Johnson, R.D., Rains, B.M., and Nepal, S. (2011). Analysis of Citalopram and Desmethylcitalopram in Postmortem Fluids and Tissues Using Liquid Chromatography-Mass Spectrometry, Technical Report for Federal Aviation Administration Oklahoma City OK Civil Aerospace Medical Institute."},{"key":"ref_78","first-page":"853","article-title":"A Rapid and Sensitive HPLC-Fluorescence Method for Determination of Mirtazapine and Its two Major Metabolites in Human Plasma","volume":"13","author":"Lavasani","year":"2014","journal-title":"Iran J. Pharm. Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jscs.2010.07.001","article-title":"Rapid quantification of mirtazapine and desmethyl mirtazapine in human plasma by LC\u2013ESI-MS\/MS: Application to a bioequivalence study","volume":"15","author":"Kuchekar","year":"2011","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"S117","DOI":"10.1111\/j.1556-4029.2012.02266.x","article-title":"Stability of serotonin-selective antidepressants in sterile and decomposing liver tissue","volume":"58","author":"Butzbach","year":"2013","journal-title":"J. Forensic Sci."},{"key":"ref_81","first-page":"311","article-title":"Stability of fluoxetine in stored plasma, aqueous, and methanolic solutions determined by HPLC with UV detection","volume":"56","author":"Binsumait","year":"2001","journal-title":"Pharmazie"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1093\/anatox\/35.8.583","article-title":"Comparison of the stability of stock solutions of drugs of abuse and other drugs stored in a freezer, refrigerator, and at ambient temperature for up to one year","volume":"35","author":"Karinen","year":"2011","journal-title":"J. Anal. Toxicol."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Youssef, R.M., Abdine, H.H., Barary, M.A., and Wagih, M.M. (2016). Selective RP-HPLC method for determination of quetiapine in presence of coadministered drugs: Application for long-term stability study of quetiapine in whole bloo. Acta Chromatogr., 28.","DOI":"10.1556\/1326.2016.28.2.2"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1556-4029.2012.02280.x","article-title":"Bacterial degradation of risperidone and paliperidone in decomposing blood","volume":"58","author":"Butzbach","year":"2013","journal-title":"J. Forensic Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.forsciint.2006.05.021","article-title":"Validation of new methods","volume":"165","author":"Peters","year":"2007","journal-title":"Forensic Sci. Int."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1007\/s00216-014-7763-2","article-title":"Validation of a multi-analyte LC-MS\/MS method for screening and quantification of 87 psychoactive drugs and their metabolites in hair","volume":"406","author":"Fisichella","year":"2014","journal-title":"Anal. Bioanal. Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/20\/3636\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:28:37Z","timestamp":1760189317000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/20\/3636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,9]]},"references-count":86,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["molecules24203636"],"URL":"https:\/\/doi.org\/10.3390\/molecules24203636","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,9]]}}}