{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:55:57Z","timestamp":1762520157179,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81260506"],"award-info":[{"award-number":["81260506"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joint Foundation of Guizhou Province Department of Science and Technology and Guizhou Medical University","award":["[2015]7357"],"award-info":[{"award-number":["[2015]7357"]}]},{"name":"Excellent Scientific and Technological Young Talent of Guizhou Province","award":["[2015]32"],"award-info":[{"award-number":["[2015]32"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["201610660034"],"award-info":[{"award-number":["201610660034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Acetaminophen (APAP) overdose is very common worldwide and has been widely recognized as the leading cause of drug-induced liver injury in the Western world. In our previous investigation, auriculatone, a natural product firstly obtained from Aster auriculatus, has demonstrated a potent protective effect against APAP-induced hepatotoxicity in HL-7702 cells. However, the poor water solubility and low bioavailability restrict its application. Auriculatone sulfate (AS) is a sulfated derivative of auriculatone with highly improved water-solubility. Hepatoprotective effects against APAP-induced liver injury (AILI) showed that intragastric pretreatment with AS at 50 mg\/kg almost completely prevented mice against APAP-induced increases of serum alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and ATPase. Histological results showed that AS could protect the liver tissue damage. In addition, AS pretreatment not only significantly retained hepatic malondialdehyde and the activities of glutathione, superoxide dismutase, and glutathione peroxidase at normal levels, but also markedly suppressed the increase of pro-inflammatory cytokines TNF-\u03b1, IL-1\u03b2, and IL-6 levels in mouse liver caused by overdose APAP. Immunohistochemical analysis showed that AS obviously attenuated the expression of CD45 and HNE in liver tissue. Further mechanisms of action investigation showed that inhibition of cytochrome P450 3A11 (CYP 3A11) and CYP2E1 enzymatic activities (but not that of CYP1A2) was responsible for APAP bioactivation. In conclusion, AS showed a hepatoprotective effect against AILI through alleviating oxidative stress and inflammation and inhibiting CYP-mediated APAP bioactivation. It may be an effective hepatoprotective agent for AILI and other forms of human liver disease.<\/jats:p>","DOI":"10.3390\/molecules24203642","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T11:25:57Z","timestamp":1570620357000},"page":"3642","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Auriculatone Sulfate Effectively Protects Mice Against Acetaminophen-Induced Liver Injury"],"prefix":"10.3390","volume":"24","author":[{"given":"Liangcai","family":"Lin","sequence":"first","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; School of Pharmacy, Guizhou Medical University, Guizhou 550025, China"},{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanyu","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; School of Pharmacy, Guizhou Medical University, Guizhou 550025, China"},{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; School of Pharmacy, Guizhou Medical University, Guizhou 550025, China"},{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangming","family":"Liao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; School of Pharmacy, Guizhou Medical University, Guizhou 550025, China"},{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifei","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; 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School of Pharmacy, Guizhou Medical University, Guizhou 550025, China"},{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun","family":"He","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou 550004, China"},{"name":"School of Medicine and Health Management, Guizhou Medical University, Guizhou 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinjuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Guizhou Medical University, Guizhou 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0479-6259","authenticated-orcid":false,"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"},{"name":"Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonglin","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guizhou 550004, China"},{"name":"Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou 550004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants &amp; 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