{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:42:06Z","timestamp":1788576126081,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:00:00Z","timestamp":1574208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Leishmaniasis is a group of neglected tropical diseases caused by protozoan parasites of the Leishmania genus. The absence of effective vaccines and the limitations of current treatments make the search for effective therapies a real need. Different plant-derived essential oils (EOs) have shown antileishmanial effects, in particular from Bixa orellana L. (EO-Bo) and Dysphania ambrosioides (L.) Mosyakin &amp; Clemants (EO-Da). In the present study, the EO-Bo and EO-Da, formulated in nanocochleates (EO-Bo-NC and EO-Da-NC, respectively), were evaluated in vitro and in vivo against L. amazonensis. The EO-Bo-NC and EO-Da-NC did not increase the in vitro inhibitory activity of the EOs, although the EO-Bo-NC showed reduced cytotoxic effects. In the animal model, both formulations (30 mg\/kg\/intralesional route\/every 4 days\/4 times) showed no deaths or weight loss greater than 10%. In the animal (mouse) model, EO-Bo-NC contributed to the control of infection (p &lt; 0.05) in comparison with EO-Bo treatment, while the mice treated with EO-Da-NC exhibited larger lesions (p &lt; 0.05) compared to those treated with EO-Da. The enhanced in vivo activity observed for EO-Bo-NC suggests that lipid-based nanoformulations like nanocochleates should be explored for their potential in the proper delivery of drugs, and in particular, the delivery of hydrophobic materials for effective cutaneous leishmaniasis treatment.<\/jats:p>","DOI":"10.3390\/molecules24234222","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T11:06:03Z","timestamp":1574247963000},"page":"4222","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Bixa orellana L. (Bixaceae) and Dysphania ambrosioides (L.) Mosyakin &amp; Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against Leishmania amazonensis"],"prefix":"10.3390","volume":"24","author":[{"given":"Laura","family":"Mach\u00edn","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Tamargo","sequence":"additional","affiliation":[{"name":"Department of Physiological Science, Latin American School of Medical Sciences, Havana 11300, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel","family":"Pi\u00f1\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine Pedro Kour\u00ed, Havana 17100, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regla C.","family":"At\u00edes","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Scull","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-0528","authenticated-orcid":false,"given":"William N.","family":"Setzer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA"},{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"},{"name":"Research Network: Natural Products against Neglected Diseases (ResNet NPND)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianet","family":"Monzote","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine Pedro Kour\u00ed, Havana 17100, Cuba"},{"name":"Research Network: Natural Products against Neglected Diseases (ResNet NPND)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13071-017-2028-5","article-title":"The history of leishmaniasis","volume":"10","author":"Steverding","year":"2017","journal-title":"Parasit. 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