{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:31:34Z","timestamp":1761611494341,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["GIU16\/04"],"award-info":[{"award-number":["GIU16\/04"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["CTQ2013-46179-R"],"award-info":[{"award-number":["CTQ2013-46179-R"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Dried blood spot (DBS) has lately experienced an increase in its use in bioanalysis due to its several advantages compared with traditional blood sampling methods. Nevertheless, the use of DBS with quantitative purposes is hindered by the heterogeneous distribution of some compounds in the supporting matrix and the dependence of the response on different factors, such as the hematocrit, blood volume, and sampling position. In this study the effect of those factors in the analytical response was investigated by ultra high performance liquid chromatography coupled to fluorescence detection, using amiloride and propranolol as model compounds. The results showed a heterogeneous and drug-dependent distribution of the compounds in the blood spot. While amiloride concentration was higher in the center, propranolol concentration was higher in the periphery of the spot. Besides, the influence of the hematocrit on the quantitative results was observed. MALDI mass spectrometry imaging (MALDI-IMS) has allowed study of the distribution of the two cardiovascular drugs when they were placed in the DBS card using water:methanol solutions, demonstrating that they followed a similar distribution pattern as in blood. This work has showed the potentiality of the MALDI-IMS technique to predict the distribution of the drugs in the DBS card.<\/jats:p>","DOI":"10.3390\/molecules24234320","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:57:27Z","timestamp":1574765847000},"page":"4320","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of the Heterogeneous Distribution of Amiloride and Propranolol in Dried Blood Spot by UHPLC-FLD and MALDI-IMS"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4048-0606","authenticated-orcid":false,"given":"Beatriz","family":"Uribe","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oskar","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Encarnaci\u00f3n","family":"Blanco","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3663-6794","authenticated-orcid":false,"given":"Oihane Elena","family":"Alb\u00f3niga","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Luz","family":"Alonso","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9046-6602","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"Alonso","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), Sarriena s\/n, 48940 Leioa, Biscay, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.forsciint.2014.04.015","article-title":"Simplifying sample pretreatment: Application of dried blood spot (DBS) method to blood samples, including postmortem, for UHPLC-MS\/MS analysis of drugs of abuse","volume":"243","author":"Odoardi","year":"2014","journal-title":"Forensic Sci. 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