{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T19:20:43Z","timestamp":1777317643774,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,29]],"date-time":"2019-12-29T00:00:00Z","timestamp":1577577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020","doi-asserted-by":"publisher","award":["633589"],"award-info":[{"award-number":["633589"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Testing and screening of plant-derived molecules on normal human cells in vitro is a widely used approach for discovering their eventual health beneficial effects for human ageing and longevity. As little is known about age-associated differential effects of such molecules, here we report that young (&lt;25% replicative lifespan completed) and near-senescent (&gt;90% replicative lifespan completed) human skin fibroblasts exposed for 1\u201315 days to a wide range of concentrations (0.1\u2013100 \u03bcM) of the three selected phytochemicals, namely \u03b1-boswellic acid acetate (ABC), praeruptorin-A (PTA), and salvianolic acid-B (SAB) had age-related differential effects. The parameters studied were the metabolic activity (MTT assay), cellular morphological phenotype, one-step growth characteristics, expression of genes involved in the cell cycle regulation and cytokine network genes, protein levels of p53, cytosolic superoxide dismutase (SOD1) and microtubule-associated protein 1A\/1B-light chain 3 (LC3), and the extent of protein carbonylation and protein aggregation as a sign of oxidative stress. All three compounds showed biphasic hormetic dose response by stimulating cell growth, survival and metabolic activity at low doses (up to 1 \u03bcM), while showing inhibitory effects at high doses (&gt;10 \u03bcM). Furthermore, the response of early passage young cells was different from that of the late passage near-senescent cells, especially with respect to the expression of cell cycle-related and inflammation-related genes. Such studies have importance with respect to the use of low doses of such molecules as health-promoting and\/or ageing-interventions through the phenomenon of hormesis.<\/jats:p>","DOI":"10.3390\/molecules25010141","type":"journal-article","created":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T05:49:41Z","timestamp":1577684981000},"page":"141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Plant-Derived Molecules \u03b1-Boswellic Acid Acetate, Praeruptorin-A, and Salvianolic Acid-B Have Age-Related Differential Effects in Young and Senescent Human Fibroblasts In Vitro"],"prefix":"10.3390","volume":"25","author":[{"given":"Anna","family":"Lewinska","sequence":"first","affiliation":[{"name":"Department of Biotechnology, University of Rzeszow, 35-310 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshman","family":"Sodagam","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Ageing, Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominika","family":"Bloniarz","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Rzeszow, 35-310 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"Siems","sequence":"additional","affiliation":[{"name":"AnalytiCon Discovery GmbH, 14473 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Wnuk","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Rzeszow, 35-310 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suresh I. S.","family":"Rattan","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Ageing, Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1089\/rej.2010.1043","article-title":"The role of polyphenolic antioxidants in health, disease, and aging","volume":"13","author":"Obrenovich","year":"2010","journal-title":"Rejuvenation Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/nyas.13399","article-title":"Understanding adaptogenic activity: Specificity of the pharmacological action of adaptogens and other phytochemicals","volume":"1401","author":"Panossian","year":"2017","journal-title":"Ann. N. Y. Acad. 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