{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T18:48:37Z","timestamp":1775414917868,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T00:00:00Z","timestamp":1577750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Currently, there is no doubt surrounding a theory that the cardiotropic effects of sex hormones can be due to their direct effect on the cardiovascular system. In recent years, interest in the study of steroid glycosides has increased. We studied the effects of furostanol glycosides (protodioscin and deltozid) from the cell culture of the Dioscorea deltoidea (laboratory code DM-05) on the physiological and biochemical parameters of vascular endothelial function in hypoestrogen-induced endothelial dysfunction after bilateral ovariectomy. It was shown that the use of DM-05 at a dose of 1 mg\/kg makes it possible to prevent the development of arterial hypertension (the level of systolic blood pressure (SBP) decreases by 9.7% (p &lt; 0.05) and diastolic blood pressure (DBP) by 8.2%), to achieve a decrease in the coefficient of endothelial dysfunction by 1.75 times against the background of a hypoestrogenic state. With DM-05, an increase in the concentration of stable nitric oxide metabolites (NOx) by 45.6% (p &lt; 0.05) and an increase in mRNA endothelial nitric oxide synthase (eNOS) expression by 34.8% (p &lt; 0.05) was established, which indicates a positive effect of furostanol glycosides on the metabolism of nitric oxide after ovariectomy. Positive dynamics in the histological structure of the heart and the abdominal aorta indicate the pronounced endothelio- and atheroprotective effects of DM-05.<\/jats:p>","DOI":"10.3390\/molecules25010169","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T07:40:55Z","timestamp":1577778055000},"page":"169","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Studies to Elucidate the Effects of Furostanol Glycosides from Dioscorea deltoidea Cell Culture in a Rat Model of Endothelial Dysfunction"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5402-0697","authenticated-orcid":false,"given":"Mikhail","family":"Korokin","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg","family":"Gudyrev","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Gureev","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliya","family":"Korokina","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Peresypkina","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Pokrovskaia","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina","family":"Lazareva","sequence":"additional","affiliation":[{"name":"Department of obstetrics and gynecology FPE, Kursk State Medical University, 305000 Kursk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Soldatov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariya","family":"Zatolokina","sequence":"additional","affiliation":[{"name":"Department of histology, embryology, cytology, Kursk State Medical University, 305000 Kursk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail","family":"Pokrovskii","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacology, Institute of medicine, Belgorod State National Research University, 308015 Belgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vph.2017.05.005","article-title":"Oxidative stress and reactive oxygen species in endothelial dysfunction associated with cardiovascular and metabolic diseases","volume":"100","author":"Incalza","year":"2017","journal-title":"Vasc. 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