{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T01:52:39Z","timestamp":1778723559519,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Special Training Program for Outstanding Scientists of the Beijing Academy of Agricultural and Forestry Sciences, grant number","award":["JKZX201908"],"award-info":[{"award-number":["JKZX201908"]}]},{"name":"Collaborative Innovation Center of the Beijing Academy of Agricultural and Forestry Sciences","award":["KJCX201915"],"award-info":[{"award-number":["KJCX201915"]}]},{"name":"the Efficient Ecological Agriculture Innovation Project of the Taishan Industry Leading Talent Program, Shandong Province","award":["LJNY201705"],"award-info":[{"award-number":["LJNY201705"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Yam yellow pigments (YP) are natural pigments formed during the storage of freshly cut yam (Dioscorea opposita) under certain conditions. The interaction of YP with calf thymus DNA (ctDNA) and its protective effect against DNA oxidative damage were investigated using multiple spectroscopic techniques, competitive binding experiments, viscosity measurements, and gel electrophoresis. Results showed that YP participated in intercalative binding with ctDNA. YP exhibited a protective effect against hydroxyl-induced DNA damage, which was attributed to the high hydroxyl radical scavenging activity of YP. Our findings improve our understanding of the mechanism of interaction between YP and ctDNA, and provide a theoretical basis for the application of YP in the food and drug industry.<\/jats:p>","DOI":"10.3390\/molecules25010175","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T11:55:07Z","timestamp":1578052507000},"page":"175","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)"],"prefix":"10.3390","volume":"25","author":[{"given":"Lei","family":"Zhao","sequence":"first","affiliation":[{"name":"Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and rural affairs, Beijing 100097, China"},{"name":"College of Food Science, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and rural affairs, Beijing 100097, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Ma","sequence":"additional","affiliation":[{"name":"Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and rural affairs, Beijing 100097, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Longda Food Group Company Limited, Shandong, Jinan 265231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and rural affairs, Beijing 100097, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"\u0141odyga-Chru\u015bci\u0144ska, E., Kowalska-Baron, A., B\u0142azi\u0144ska, P., Pilo, M., Zucca, A., Korolevich, V.M., and Cheshchevik, V.T. (2019). Position impact of hydroxy groups on spectral, acid\u2013base profiles and DNA interactions of several monohydroxy flavanones. Molecules, 24.","DOI":"10.3390\/molecules24173049"},{"key":"ref_2","first-page":"259","article-title":"Evaluation of the DNA damage protective activity of the germinated peanut (Arachis hypogaea) in relation to antioxidant and anti-inflammatory activity","volume":"101","author":"Limmongkon","year":"2019","journal-title":"Food Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Liu, B., Jin, S.F., Li, H.-C., Sun, X.-Y., Yan, S.-Q., Deng, S.-J., and Zhao, P. (2019). The Bio-safety concerns of three domestic temporary hair dye molecules: Fuchsin Basic, Victoria Blue B and Basic Red 2. Molecules, 24.","DOI":"10.3390\/molecules24091744"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Bravo-Anaya, L., Roux, D., Soltero Mart\u00ednez, J., Carvajal Ramos, F., Pignon, F., Mannix, O., and Rinaudo, M. (2018). Role of electrostatic interactions on supramolecular organization in calf-thymus DNA Solutions under Flow. Polymers, 10.","DOI":"10.3390\/polym10111204"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1111\/pce.13428","article-title":"Apigenin produced by maize flavone synthase I and II protects plants against UV-B-induced damage","volume":"42","author":"Righini","year":"2019","journal-title":"Plant Cell Environ."},{"key":"ref_6","first-page":"1200","article-title":"A new function of Chinese bayberry extract: Protection against oxidative DNA damage","volume":"60","author":"Chen","year":"2015","journal-title":"Food Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.jchromb.2017.11.044","article-title":"Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry","volume":"1072","author":"Aybastler","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jnutbio.2004.05.005","article-title":"Ochratoxin A-induced DNA damage in human fibroblast: Protective effect of cyanidin 3-O-\u03b2-d-glucoside","volume":"16","author":"Russo","year":"2005","journal-title":"J. Nutr. Biochem."},{"key":"ref_9","unstructured":"Lei, Z., Dan, W., Yue, M., Yan, Z., and Xiaoyan, Z. (2019). Yellow pigment formation, pigment composition, and quality of fresh-cut yam (Dioscorea opposita) slices. RSC Adv., accepted."},{"key":"ref_10","first-page":"177","article-title":"Spectroscopic studies of the effects of anticancer drug mitoxantrone interaction with calf-thymus DNA","volume":"120","author":"Agarwal","year":"2013","journal-title":"J. Food Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ijbiomac.2014.10.017","article-title":"Interaction of coumarin with calf thymus DNA: Deciphering the mode of binding by in vitro studies","volume":"73","author":"Sarwar","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Sirajuddin, M., Ali, S., and Badshah, A. (2013). Drug-DNA interactions and their study by UV-Visible, fluorescence spectroscopies and cyclic voltametry. J. Photochem. Photobiol. B-Biol., 124C.","DOI":"10.1016\/j.jphotobiol.2013.03.013"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1016\/j.bmc.2012.03.006","article-title":"Synthesis of novel 9-O-N-aryl\/aryl-alkyl amino carbonyl methyl substituted berberine analogs and evaluation of DNA binding aspects","volume":"20","author":"Basu","year":"2012","journal-title":"Bioorg Med Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.ica.2018.02.035","article-title":"DNA interaction analysis of fluorenylidene double bridged cyclotriphosphazene derivatives","volume":"477","year":"2018","journal-title":"Inorg. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jphotobiol.2011.12.011","article-title":"Spectroscopic studies on the interaction between carbaryl and calf thymus DNA with the use of ethidium bromide as a fluorescence probe","volume":"108","author":"Zhang","year":"2012","journal-title":"J. Photochem. Photobiol. B-Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1080\/10942912.2010.489209","article-title":"Color characteristics of carrots: Effect of drying and rehydration","volume":"15","author":"Zielinska","year":"2012","journal-title":"Int. J. Food Prop."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.jlumin.2018.10.106","article-title":"Positive curvature in Stern-Volmer plot described by a generalized model for static quenching","volume":"206","author":"Ciotta","year":"2019","journal-title":"J. Lumines"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ijbiomac.2006.03.020","article-title":"Studies on the interaction between Oxaprozin-E and bovine serum albumin by spectroscopic methods","volume":"39","author":"Sun","year":"2006","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.foodcont.2019.03.031","article-title":"Membrane disruption and DNA binding of Fusarium graminearum cell induced by C16-Fengycin A produced by Bacillus amyloliquefaciens","volume":"102","author":"Liu","year":"2019","journal-title":"Food Control."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.snb.2009.10.060","article-title":"Study of interaction between kaempferol\u2013Eu3+ complex and DNA with the use of the Neutral Red dye as a fluorescence probe","volume":"144","author":"Zhang","year":"2010","journal-title":"Sens. Actuator B-Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/j.elecom.2004.09.001","article-title":"Electrochemical investigation of neutral red binding to DNA at the surface","volume":"6","author":"Heli","year":"2004","journal-title":"Electrochem. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/j.talanta.2004.09.008","article-title":"Synchronous fluorescence and UV-vis spectrometric study of the competitive interaction of chlorpromazine hydrochloride and Neutral Red with DNA using chemometrics approaches","volume":"65","author":"Yongnian","year":"2005","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.molliq.2018.02.031","article-title":"Probing the binding of lomefloxacin to a calf thymus DNA-histone H1 complex by multi-spectroscopic and molecular modeling techniques","volume":"256","author":"Sohrabi","year":"2018","journal-title":"J. Mol. Liq."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.aca.2006.11.006","article-title":"Interaction between quercetin-copper (II) complex and DNA with the use of the Neutral Red dye fluorophor probe","volume":"584","author":"Ni","year":"2007","journal-title":"Anal. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jphotobiol.2014.10.011","article-title":"Intercalation binding of food antioxidant butylated hydroxyanisole to calf thymus DNA","volume":"141","author":"Wang","year":"2014","journal-title":"J. Photochem. Photobiol. B-Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.poly.2019.01.052","article-title":"DNA binding and molecular docking studies of a new Cu(II) complex of isoxsuprine drug","volume":"162","author":"Hajibabaei","year":"2019","journal-title":"Polyhedron"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1016\/j.jlumin.2009.06.010","article-title":"Study of interaction of a fluorescent probe with DNA","volume":"129","author":"Wu","year":"2009","journal-title":"J. Lumines"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s00217-010-1226-6","article-title":"In vitro studies on calf thymus DNA interaction and 2-tert-butyl-4-methylphenol food additive","volume":"230","author":"Kashanian","year":"2010","journal-title":"Eur. Food Res. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2638","DOI":"10.1021\/jf400017f","article-title":"Probing the binding of insecticide permethrin to calf thymus DNA by spectroscopic techniques merging with chemometrics method","volume":"61","author":"Zhang","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1002\/ejic.200300718","article-title":"Nucleobase oxidation of DNA by (Terpyridyl)chromium(III) derivatives","volume":"2004","author":"Vaidyanathan","year":"2010","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.molliq.2019.03.018","article-title":"Interaction of isoeugenol with calf thymus DNA and its protective effect on DNA oxidative damage","volume":"282","author":"Wang","year":"2019","journal-title":"J. Mol. Liq."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zhang, G., Wang, L.-H., Zhou, X., Li, Y., and Gong, D. (2014). Binding Characteristics of Sodium Saccharin with calf thymus DNA in vitro. J. Agric. Food Chem., 62.","DOI":"10.1021\/jf405085g"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ab.2013.03.014","article-title":"A hydroxyl-scavenging assay based on DNA damage in vitro","volume":"438","author":"Li","year":"2014","journal-title":"Anal. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"H889","DOI":"10.1111\/1750-3841.12823","article-title":"Binding characteristics and protective capacity of cyanidin-3-glucoside and its aglycon to calf thymus DNA","volume":"80","author":"Zhang","year":"2015","journal-title":"J. Food Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s12957-018-1400-z","article-title":"Quercetin inhibits prostate cancer by attenuating cell survival and inhibiting anti-apoptotic pathways","volume":"16","author":"Ward","year":"2018","journal-title":"World J. Surg. Oncol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ibtissam, T., Matkarimov, B., Tch\u00e9nio, T., Zharkov, D., and Saparbaev, M. (2016). Aberrant base excision repair pathway of oxidatively damaged DNA: Implications for degenerative diseases. Free Radic. Biol. Med., 107.","DOI":"10.1016\/j.freeradbiomed.2016.11.040"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jinorgbio.2015.08.015","article-title":"In vitro antitumor activity of free and nano-encapsulated Na5[PMo10V2O40]\u00b7nH2O and its binding properties with ctDNA by using combined spectroscopic methods","volume":"152","author":"Dianat","year":"2015","journal-title":"J. Inorg. Biochem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Amado, A., Pazin, W., Ito, A., Kuzmin, V., and Borissevitch, I. (2017). Acridine orange interaction with DNA: Effect of ionic strength. Biochim. Biochim. Biophys. Acta-Gen. Subj., 1861.","DOI":"10.1016\/j.bbagen.2017.01.023"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.ijbiomac.2014.12.018","article-title":"Groove binding interaction between daphnetin and calf thymus DNA","volume":"74","author":"Zhou","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.jscs.2014.06.004","article-title":"Flos chrysanthemi indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism","volume":"19","author":"Li","year":"2015","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jpba.2018.08.033","article-title":"In vitro binding interaction of atorvastatin with calf thymus DNA: Multispectroscopic, gel electrophoresis and molecular docking studies","volume":"161","year":"2018","journal-title":"J Pharm Biomed Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.foodchem.2004.11.043","article-title":"Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models","volume":"94","author":"Mathew","year":"2006","journal-title":"Food Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/1\/175\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:03:27Z","timestamp":1760364207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/1\/175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,1]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["molecules25010175"],"URL":"https:\/\/doi.org\/10.3390\/molecules25010175","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,1]]}}}