{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:31:32Z","timestamp":1790724692259,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:00:00Z","timestamp":1578268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["UMO 2016\/23\/B\/NZ4\/01379"],"award-info":[{"award-number":["UMO 2016\/23\/B\/NZ4\/01379"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Polish Ministry of Science and Higher Education","award":["IP2015 048474"],"award-info":[{"award-number":["IP2015 048474"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This work focused on a detailed assessment of lung tissue affected by metastasis of breast cancer. We used large-area chemical scanning implemented in Fourier transform infrared (FTIR) spectroscopic imaging supported with classical histological and morphological characterization. For the first time, we differentiated and defined biochemical changes due to metastasis observed in the lung parenchyma, atelectasis, fibrous, and muscle cells, as well as bronchi ciliate cells, in a qualitative and semi-quantitative manner based on spectral features. The results suggested that systematic extracellular matrix remodeling with the progress of the metastasis process evoked a decrease in the fraction of the total protein in atelectasis, fibrous, and muscle cells, as well as an increase of fibrillar proteins in the parenchyma. We also detected alterations in the secondary conformations of proteins in parenchyma and atelectasis and changes in the level of hydroxyproline residues and carbohydrate moieties in the parenchyma. The results indicate the usability of FTIR spectroscopy as a tool for the detection of extracellular matrix remodeling, thereby enabling the prediction of pre-metastatic niche formation.<\/jats:p>","DOI":"10.3390\/molecules25010236","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T10:34:46Z","timestamp":1578306886000},"page":"236","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Tracking Extracellular Matrix Remodeling in Lungs Induced by Breast Cancer Metastasis. Fourier Transform Infrared Spectroscopic Studies"],"prefix":"10.3390","volume":"25","author":[{"given":"Karolina","family":"Chrabaszcz","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Kaminska","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 30-384 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Augustyniak","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-4239","authenticated-orcid":false,"given":"Monika","family":"Kujdowicz","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland"},{"name":"Department of Pathology, Medical Faculty, Jagiellonian University Medical College, 31-531 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Smeda","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 30-384 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Jasztal","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 30-384 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Stojak","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 30-384 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8098-4622","authenticated-orcid":false,"given":"Katarzyna M.","family":"Marzec","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 30-384 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0582-2743","authenticated-orcid":false,"given":"Kamilla","family":"Malek","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/nrc2627","article-title":"Parallel progression of primary tumours and metastases","volume":"9","author":"Klein","year":"2009","journal-title":"Nat. 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