{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T19:01:46Z","timestamp":1774638106927,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T00:00:00Z","timestamp":1578528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772278 and 31300285"],"award-info":[{"award-number":["81772278 and 31300285"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Educational and Scientific Research Project for Young and Middle-aged Teachers of the Fujian Province","award":["JT180012"],"award-info":[{"award-number":["JT180012"]}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2015J01348"],"award-info":[{"award-number":["2015J01348"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["20720170105"],"award-info":[{"award-number":["20720170105"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Traditional Chinese medicine (TCM)has played an important role in promoting the health of Chinese people. The TCM Psoralea corylifolia L. has been used in the treatment of various kinds of diseases including enuresis, vitiligo, and calvities. However, therapeutic effects of P. corylifolia L. have often influenced by the quality of plants. So, it is very important to control the quality of P. corylifolia L. In this study, analytical high-speed countercurrent chromatography (HSCCC) was successfully used to fingerprint P. corylifolia L. Samples of P. corylifolia L. were extracted by ultrasonic extraction. n-hexane-ethyl acetate\u2013methanol\u2013water at a ratio of 5:5.5:6.5:5 (v\/v) was selected as a two-phase solvent system and the condition of HSCCC were optimized in order to good separation. And the method of HSCCC was verified (reproducibility, precision, and stability). HSCCC chromatograms exhibited six common peaks, which were selected as indicator compounds for the quality control of P. corylifolia L. Within 20 types of medicinal materials, chemical components are similar, but the levels of components are quite different in HSCCC fingerprint. The present results demonstrate that the HSCCC method provides a reliable basis for the quality control of P. corylifolia L. and can also be applied to confirm the authenticity of plant materials.<\/jats:p>","DOI":"10.3390\/molecules25020279","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T04:06:51Z","timestamp":1578629211000},"page":"279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Quality Control of Psoralea corylifolia L. Based on High-Speed Countercurrent Chromatographic Fingerprinting"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6409-6687","authenticated-orcid":false,"given":"Xiaoxue","family":"Wu","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Gao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinmei","family":"Liu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huayu","family":"Yang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Song","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Funan","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.foodchem.2004.04.029","article-title":"Antioxidants from a Chinese medicinal herb\u2013Psoralea corylifolia L.","volume":"91","author":"Guo","year":"2005","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"363052","DOI":"10.1093\/ecam\/nen087","article-title":"Screening antitumor compounds psoralen and isopsoralen from Psoralea corylifolia L. seeds","volume":"2011","author":"Wang","year":"2010","journal-title":"Evid-Based Compl. Alt."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"12500","DOI":"10.3390\/molecules200712500","article-title":"Constituents of Psoralea corylifolia Fruits and their effects on methicillin-resistant staphylococcus aureus","volume":"20","author":"Cui","year":"2015","journal-title":"Molecules"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1016\/j.tet.2005.12.041","article-title":"Psoracorylifols A\u2013E, five novel compounds with activity against Helicobacter pylori from seeds of Psoralea corylifolia","volume":"62","author":"Yin","year":"2006","journal-title":"Tetrahedron"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1002\/tox.22564","article-title":"The ethanol extraction of prepared Psoralea corylifolia induces apoptosis and autophagy and alteres genes expression assayed by cDNA microarray in human prostate cancer PC-3 cells","volume":"33","author":"Lin","year":"2018","journal-title":"Environ. Toxicol."},{"key":"ref_6","first-page":"192","article-title":"P-glycoprotein on blood-brain barrier plays a vital role in fentanyl brain exposure and respiratory toxicity in rats","volume":"33","author":"Yu","year":"2018","journal-title":"Toxicol. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/10286020500289618","article-title":"Studies on the chemical constituents of Psoralea corylifolia L.","volume":"9","author":"Ruan","year":"2007","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Li, J., Chen, Y., Han, L., and Liu, K. (2018). The role of hepatic antioxidant capacity and hepatobiliary transporter in liver injury induced by isopsoralen in zebrafish larvae. Hum. Exp. Toxicol., 38.","DOI":"10.1177\/0960327118774873"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4387","DOI":"10.1016\/j.bmc.2004.06.014","article-title":"Antibacterial prenylflavone derivatives from Psoralea corylifolia, and their structure-activity relationship study","volume":"12","author":"Yin","year":"2004","journal-title":"Bioorgan. Med. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1055\/s-2001-18343","article-title":"Osteoblastic proliferation stimulating activity of Psoralea corylifolia extracts and two of its flavonoids","volume":"67","author":"Wang","year":"2001","journal-title":"Planta Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"943","DOI":"10.2174\/138620710793360310","article-title":"Chromatographic fingerprinting and metabolomics for quality control of TCM","volume":"13","author":"Liang","year":"2010","journal-title":"Comb. Chem. High Throughput Scr."},{"key":"ref_12","first-page":"172","article-title":"Effect of fingerprintology of traditional Chinese medicine (TCM) in the innovative development of TCM","volume":"26","author":"Sun","year":"2008","journal-title":"Chin. J. Chromatogr."},{"key":"ref_13","first-page":"19","article-title":"Research Progress on fingerprinting of traditional Chinese medicine","volume":"5","author":"Junhong","year":"2009","journal-title":"J. Agric. Sci."},{"key":"ref_14","first-page":"572","article-title":"Application of chromatography in fingerprint study of Chinese traditional medicine","volume":"21","author":"Chaoshen","year":"2003","journal-title":"Chin. J. Chromatogr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.sajb.2009.02.002","article-title":"Identification and classification of Dendrobium candidum species by fingerprint technology with capillary electrophoresis","volume":"75","author":"Zha","year":"2009","journal-title":"S. Afr. J. Bot."},{"key":"ref_16","first-page":"23","article-title":"Evaluation of flavonoid and polyphenol constituents in mulberry leaves using HPLC fingerprint analysis","volume":"19","author":"He","year":"2019","journal-title":"Int. J. Food Sci. Tech."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.indcrop.2018.05.062","article-title":"A combination of simultaneous quantification of four triterpenes and fingerprint analysis using HPLC for rapid identification of Centella asiatica from its related plants and classification based on cultivation ages","volume":"122","author":"Rafi","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_18","first-page":"643","article-title":"GC-MS-olfactometric to control the aroma fingerprint of extra-virgin olive oil from three tunisian cultivars at three harvest time","volume":"66","author":"Brahim","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","unstructured":"Qian, H.Q., Li, C.J., and Xie, P.S. (2001, April 03). On GC Fingerprint of Rhizome of Aplinia Officinarum (Gao Liang Jiang) and Its Congeners. Available online: http:\/\/wprim.whocc.org.cn\/admin\/article\/articleDetail?WPRIMID=569867&articleId=569867."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1126\/science.167.3916.281","article-title":"Countercurrent chromatography: Liquid-liquid partition chromatography without solid support","volume":"167","author":"Ito","year":"1970","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.bjp.2017.01.003","article-title":"Isolation of quinoline alkaloids from three choisya species by high-speed countercurrent chromatography and the determination of their antioxidant capacity","volume":"27","author":"Pereira","year":"2017","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.chroma.2008.09.116","article-title":"Preparative separation of a terpenoid and alkaloids from Tripterygium wilfordii Hook. f. using high-performance counter-current chromatography: Comparison of various elution and operating strategies","volume":"1213","author":"Ye","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"ref_23","first-page":"885","article-title":"Separation offlavonoids from the Leaves of Oroxylum indicum by HSCCC","volume":"68","author":"Yuan","year":"2008","journal-title":"Sutherland"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Guo, W., Dong, H.J., Wang, D.J., Yang, B., Wang, X., and Huang, L.Q. (2018). Separation of seven polyphenols from the rhizome of Smilax glabra by offline two dimensions recycling HSCCC with extrusion mode. Molecules, 23.","DOI":"10.3390\/molecules23020505"},{"key":"ref_25","first-page":"1770","article-title":"Progress in predicting animal feed intake of plant secondary compounds by spectral analysis","volume":"27","author":"Wang","year":"2007","journal-title":"J. Guang Pu."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.chroma.2012.11.029","article-title":"Preparative isolation and purification of macrolactin antibiotics from marine bacterium Bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode","volume":"1272","author":"He","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.seppur.2010.08.007","article-title":"Separation of minor coumarins from Peucedanum praeruptorum using HSCCC and preparative HPLC guided by HPLC\/MS","volume":"75","author":"Hou","year":"2010","journal-title":"Sep. Purif. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1002\/jsfa.7451","article-title":"Isolation of cytotoxic diterpenoids from the Chilean medicinal plant Azorella compacta Phil from the Atacama Desert by high-speed counter-current chromatography","volume":"96","author":"Bartolucci","year":"2016","journal-title":"J. Sci. Food Agric."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/2\/279\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:04:22Z","timestamp":1760364262000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/2\/279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,9]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["molecules25020279"],"URL":"https:\/\/doi.org\/10.3390\/molecules25020279","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,9]]}}}