{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:32:37Z","timestamp":1792017157464,"version":"4.4.0"},"reference-count":53,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pleurotus eryngii (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of P. eryngii is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elements with a functional activity in the human body. In this study, the ability of P. eryngii mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of P. eryngii was determined as well. To enhance Se and Zn content in P. eryngii fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L\u22121, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L\u22121, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20 mg L\u22121. The content of organic compounds include phenolic compounds and lovastatin, which were determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of P. eryngii to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be different depending on the type of salt used. The present study also showed that conducting mycelium of P. eryngii in in vitro culture, with a higher content of zinc ions, can result in obtaining the materials with better antioxidant ability. The results of this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements.<\/jats:p>","DOI":"10.3390\/molecules25040889","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T05:44:12Z","timestamp":1582695852000},"page":"889","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Selenium and Zinc Biofortification of Pleurotus eryngii Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity"],"prefix":"10.3390","volume":"25","author":[{"given":"Piotr","family":"Zi\u0119ba","sequence":"first","affiliation":[{"name":"Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Ka\u0142a","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5121-467X","authenticated-orcid":false,"given":"Anna","family":"W\u0142odarczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Szewczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Kunicki","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-5742","authenticated-orcid":false,"given":"Agnieszka","family":"S\u0119kara","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5007-1486","authenticated-orcid":false,"given":"Bo\u017cena","family":"Muszy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jff.2018.12.034","article-title":"Immune-modulating activities of glucans extracted from Pleurotus ostreatus and Pleurotus eryngii","volume":"54","author":"Vetvicka","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_2","unstructured":"Stamets, P. (2011). Growing Gourmet and Medicinal Mushrooms, Ten Speed Press. [3rd ed.]."},{"key":"ref_3","first-page":"73","article-title":"Determination of culture process for obtaining basidiocarp of Pleurotus eryngii (DC. ex Fr.) Quel. var. ferulae Lanzi: A speciality mushroom","volume":"67","author":"Kirbag","year":"2010","journal-title":"Indian J. Hortic."},{"key":"ref_4","first-page":"1","article-title":"Auricularia spp.-mushrooms as Novel Food and therapeutic agents-a review","volume":"67","author":"Sekara","year":"2015","journal-title":"Sydowia"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s00253-006-0684-z","article-title":"DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex","volume":"74","author":"Urbanelli","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1099\/00221287-147-11-3183","article-title":"Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters","volume":"147","author":"Zervakis","year":"2001","journal-title":"Microbiology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/07388550802688821","article-title":"Biology of Pleurotus eryngii and role in biotechnological processes: A review","volume":"29","author":"Vukojevic","year":"2009","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Avni, S., Ezov, N., Hanani, H., Yadid, I., Karpovsky, M., Hayby, H., Gover, O., Hadar, Y., Schwartz, B., and Danay, O. (2017). Olive Mill Waste Enhances \u03b1-Glucan Content in the Edible Mushroom Pleurotus eryngii. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18071564"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s13568-018-0678-0","article-title":"Supplementation in mushroom crops and its impact on yield and quality","volume":"8","author":"Carrasco","year":"2018","journal-title":"AMB Express"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1039\/C7FO00515F","article-title":"Development of nutraceutical formulations based on the mycelium of Pleurotus ostreatus and Agaricus bisporus","volume":"8","author":"Cardoso","year":"2017","journal-title":"Food Funct."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.22207\/JPAM.10.4.11","article-title":"Comparative Mycelial Growth of Pleurotus djamor and Pleurotus ostreatus in Culture Media","volume":"10","author":"Tello","year":"2016","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref_12","unstructured":"Souilem, F., Fernandes, \u00c2., Calhelha, R.C., Barreira, J., Barros, L., Skhiri, F., and Ferreira, I.C. (2018, January 3\u20136). Bioactive compounds and anti-inflammatory activity of Pleurotus eryngii and Suillus bellinii: A comparison between fruiting bodies and mycelia. Proceedings of the 9es Journ\u00e9es Scientifiques Internationales sur la Valorisation des Bioressources, Monastir, Tunisia."},{"key":"ref_13","first-page":"835","article-title":"Box Wilson factorial design methodology for the optimization of laccase production by Pleurotus djamor in liquid Culture","volume":"2","year":"2016","journal-title":"Curr. Res. Microbiol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s00217-015-2474-2","article-title":"Supplementation of cultivated mushroom species with selenium: Bioaccumulation and speciation study","volume":"241","author":"Niedzielski","year":"2015","journal-title":"Eur. Food Res. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1007\/s00217-015-2580-1","article-title":"Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc","volume":"242","author":"Mleczek","year":"2016","journal-title":"Eur. Food Res. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1007\/s00217-017-2921-3","article-title":"Bio-enriched Pleurotus mushrooms for deficiency control and improved antioxidative protection of human platelets?","volume":"243","author":"Mleczek","year":"2017","journal-title":"Eur. Food Res. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"66","DOI":"10.5943\/cream\/9\/1\/7","article-title":"Nutrient contents and antioxidant properties of Pleurotus spp. cultivated on substrate fortified with Selenium","volume":"9","author":"Fasoranti","year":"2019","journal-title":"Curr. Res. Environ. Appl. Mycol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"12","DOI":"10.4489\/MYCO.2011.39.1.012","article-title":"Assessment of Antioxidant and Phenolic Compound Concentrations as well as Xanthine Oxidase and Tyrosinase Inhibitory Properties of Different Extracts of Pleurotus citrinopileatus Fruiting Bodies","volume":"39","author":"Alam","year":"2011","journal-title":"Mycobiology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/978-981-10-5978-0_11","article-title":"Mushrooms: A Pandora Box of Cardioprotective Phytochemicals","volume":"Volume 4","author":"Shibu","year":"2017","journal-title":"Medicinal and Aromatic Plants of the Middle-East"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.cbi.2008.12.012","article-title":"Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats","volume":"179","author":"Priscilla","year":"2009","journal-title":"Chem. Interact."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1021\/acs.jafc.7b00715","article-title":"Mycochemical Changes Induced by Selenium Enrichment in P. ostreatus Fruiting Bodies","volume":"65","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1080\/10942912.2016.1210162","article-title":"Therapeutic properties of Pleurotus species (Oyster mushrooms) for Atherosclerosis: A review","volume":"20","author":"Abidin","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1038\/sj.leu.2402501","article-title":"The cholesterol lowering drug lovastatin induces cell death in myeloma plasma cells","volume":"16","author":"Kamphuis","year":"2002","journal-title":"Leukemia"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2478\/fhort-2018-0012","article-title":"Bioactive compounds and medicinal properties of Oyster mushrooms (Pleurotus sp.)","volume":"30","author":"Siwulski","year":"2018","journal-title":"Folia Hortic."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ijbiomac.2016.11.060","article-title":"Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var. tuoliensis","volume":"95","author":"Xu","year":"2017","journal-title":"Int. J. Boil. Macromol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0048-9697(99)00411-8","article-title":"Concentrations of mercury, cadmium, lead and copper in fruiting bodies of edible mushrooms in an emission area of a copper smelter and a mercury smelter","volume":"246","author":"Svoboda","year":"2000","journal-title":"Sci. Total. Environ."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1079\/PHN2001143","article-title":"Selenium, selenoproteins and human health: A review","volume":"4","author":"Brown","year":"2001","journal-title":"Public Health Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1002\/mnfr.200700330","article-title":"The influence of selenium on immune responses","volume":"52","author":"Hoffmann","year":"2008","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"9776","DOI":"10.1074\/jbc.M110.163964","article-title":"Presenilins Promote the Cellular Uptake of Copper and Zinc and Maintain Copper Chaperone of SOD1-dependent Copper\/Zinc Superoxide Dismutase Activity","volume":"286","author":"Greenough","year":"2011","journal-title":"J. Boil. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.3390\/ijerph7041342","article-title":"The Essential Toxin: Impact of Zinc on Human Health","volume":"7","author":"Plum","year":"2010","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/s12011-012-9454-1","article-title":"Antioxidant and Antitumor Activities of Selenium- and Zinc-Enriched Oyster Mushroom in Mice","volume":"150","author":"Yan","year":"2012","journal-title":"Boil. Trace Elem. Res."},{"key":"ref_32","first-page":"151","article-title":"Growth and Tolerance of Pleurotus ostreatus at Different Selenium Forms","volume":"11","author":"Silva","year":"2019","journal-title":"J. Agric. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1021\/jf052973u","article-title":"Se-Enriched Mycelia ofPleurotus ostreatus: Distribution of Selenium in Cell Walls and Cell Membranes\/Cytosol","volume":"54","author":"Kubachka","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1021\/jf8008553","article-title":"Phenolic Compound Concentration and Antioxidant Activities of Edible and Medicinal Mushrooms from Korea","volume":"56","author":"Kim","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1111\/1750-3841.12189","article-title":"Effects of Various Heat Treatments on Phenolic Profiles and Antioxidant Activities of Pleurotus eryngii Extracts","volume":"78","author":"Li","year":"2013","journal-title":"J. Food Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"326","DOI":"10.2306\/scienceasia1513-1874.2009.35.326","article-title":"Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju","volume":"35","author":"Chirinang","year":"2009","journal-title":"Sci. Asia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.fct.2011.10.056","article-title":"Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study","volume":"50","author":"Reis","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1615\/IntJMedMushr.v14.i2.30","article-title":"Screening of medicinal higher Basidiomycetes mushrooms from Turkey for lovastatin production","volume":"14","author":"Atli","year":"2012","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s12011-013-9710-z","article-title":"Effects of Different Selenium Levels on Gene Expression of a Subset of Selenoproteins and Antioxidative Capacity in Mice","volume":"154","author":"Zhang","year":"2013","journal-title":"Boil. Trace Elem. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1007\/s11274-014-1636-x","article-title":"Effects of different selenium levels on growth and regulation of laccase and versatile peroxidase in white-rot fungus, Pleurotus eryngii","volume":"30","author":"Kim","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Wang, J., Wang, B., Zhang, D., and Wu, Y. (2016). Selenium uptake, tolerance and reduction in Flammulina velutipes supplied with selenite. PeerJ, 4.","DOI":"10.7717\/peerj.1993"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kaur, G., Kalia, A., and Sodhi, H.S. (2018). Selenium biofortification of Pleurotus species and its effect on yield, phytochemical profiles, and protein chemistry of fruiting bodies. J. Food Biochem., 42.","DOI":"10.1111\/jfbc.12467"},{"key":"ref_43","first-page":"467","article-title":"Ability of selenium absorption by mycelia of Pleurotus eryngii (DC.: Fr.) Quel., depending on selenium source in medium","volume":"7","author":"Stajic","year":"2005","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1007\/s13197-012-0656-5","article-title":"Important nutritional constituents, flavour components, antioxidant and antibacterial properties of Pleurotus sajor-caju","volume":"51","author":"Gogavekar","year":"2014","journal-title":"J. Food Sci. Tech."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6419","DOI":"10.1021\/jf020608m","article-title":"Basic Composition and Amino Acid Contents of Mushrooms Cultivated in Finland","volume":"50","author":"Mattila","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3387","DOI":"10.1007\/s12010-015-1510-9","article-title":"Improvement of Zinc Bioaccumulation and Biomass Yield in the Mycelia and Fruiting Bodies of Pleurotus florida Cultured on Liquid Media","volume":"175","author":"Poursaeid","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.lwt.2013.06.016","article-title":"Antioxidant activities, total phenolics and metal contents in Pleurotus ostreatus mushrooms enriched with iron, zinc or lithium","volume":"54","author":"Vieira","year":"2013","journal-title":"LWT"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0308-8146(99)00264-2","article-title":"A review of trace element concentrations in edible mushrooms","volume":"69","year":"2000","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1007\/s00217-019-03299-9","article-title":"The effects of germanium and selenium on growth, metalloid accumulation and ergosterol content in mushrooms: Experimental study in Pleurotus ostreatus and Ganoderma lucidum","volume":"245","author":"Siwulski","year":"2019","journal-title":"Eur. Food Res. Technol."},{"key":"ref_50","unstructured":"U.S. Food and Drug Administration (2012, September 28). Available online: http:\/\/www.fda.gov\/."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1039\/C7FO01747B","article-title":"Circular reuse of bio-resources: The role of Pleurotus spp. in the development of functional foods","volume":"9","author":"Lavelli","year":"2018","journal-title":"Food Funct."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s11274-009-0049-8","article-title":"Enhancement of the antioxidants ergothioneine and selenium in Pleurotus eryngii var. eryngii basidiomata through cultural practices","volume":"25","author":"Estrada","year":"2009","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1080\/10942912.2016.1219742","article-title":"Kinetics of extracted bioactive components from mushrooms in artificial digestive juices","volume":"20","author":"Krakowska","year":"2017","journal-title":"Int. J. Food Prop."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/4\/889\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:58:32Z","timestamp":1760173112000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/4\/889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,17]]},"references-count":53,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["molecules25040889"],"URL":"https:\/\/doi.org\/10.3390\/molecules25040889","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,17]]}}}