{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:01:46Z","timestamp":1772550106664,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T00:00:00Z","timestamp":1582329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS 1446310"],"award-info":[{"award-number":["IOS 1446310"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006733","name":"Indiana University","doi-asserted-by":"publisher","award":["BRG"],"award-info":[{"award-number":["BRG"]}],"id":[{"id":"10.13039\/100006733","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08DK113226"],"award-info":[{"award-number":["K08DK113226"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001212","name":"Koret Foundation","doi-asserted-by":"publisher","award":["1"],"award-info":[{"award-number":["1"]}],"id":[{"id":"10.13039\/100001212","id-type":"DOI","asserted-by":"publisher"}]},{"name":"George H. Clowe\u2019s Memorial Research Career Development Award","award":["1"],"award-info":[{"award-number":["1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Manganese porphyrins (MnPs), MnTE-2-PyP5+, MnTnHex-2-PyP5+ and MnTnBuOE-2-PyP5+, are superoxide dismutase (SOD) mimetics and form a redox cycle between O2 and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H2O2). We previously found that MnPs oxidize hydrogen sulfide (H2S) to polysulfides (PS; H2Sn, n = 2\u20136) in buffer. Here, we examine the effects of MnPs for 24 h on H2S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H2S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H2S production and increased intracellular PS. H2S metabolism and PS production were unaffected by cellular O2 (5% versus 21% O2), H2O2 or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H2S production in HEK293 cells and that MnPs decrease mitochondrial H2S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H2S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.<\/jats:p>","DOI":"10.3390\/molecules25040980","type":"journal-article","created":{"date-parts":[[2020,2,27]],"date-time":"2020-02-27T03:21:16Z","timestamp":1582773676000},"page":"980","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effects of Manganese Porphyrins on Cellular Sulfur Metabolism"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0178-2203","authenticated-orcid":false,"given":"Kenneth R.","family":"Olson","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine-South Bend Center, South Bend, IN 46617, USA"},{"name":"Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine-South Bend Center, South Bend, IN 46617, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea K.","family":"Steiger","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3604-653X","authenticated-orcid":false,"given":"Michael D.","family":"Pluth","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Oregon, Eugene, OR 97403, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Tessier","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine-South Bend Center, South Bend, IN 46617, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troy A.","family":"Markel","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Riley Hospital for Children at IU Health, 705 Riley Hospital Dr, RI 2500, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Boone","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine-South Bend Center, South Bend, IN 46617, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5443-7863","authenticated-orcid":false,"given":"Robert V.","family":"Stahelin","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Batinic-Haberle","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, School of Medicine, Duke University, Durham, NC 27710, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl D.","family":"Straubg","sequence":"additional","affiliation":[{"name":"Central Arkansas Veteran\u2019s Healthcare System, Little Rock, AR 72205, USA"},{"name":"Departments of Medicine and Biochemistry, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Batinic-Haberle, I., and Tome, M.E. 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