{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:06Z","timestamp":1787029086378,"version":"build-2736575974"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A new \u03b2-class carbonic anhydrase was cloned and purified from the filamentous ascomycete Sordaria macrospora, CAS3. This enzyme has a higher catalytic activity compared to the other two such enzymes from this fungus, CAS1 and CAS2, which were reported earlier, with the following kinetic parameters: kcat of (7.9 \u00b1 0.2) \u00d7 105 s\u22121, and kcat\/Km of (9.5 \u00b1 0.12) \u00d7 107 M\u22121\u2219s\u22121. An inhibition study with a panel of sulfonamides and one sulfamate was also performed. The most effective CAS3 inhibitors were benzolamide, brinzolamide, dichlorophnamide, methazolamide, acetazolamide, ethoxzolamide, sulfanilamide, methanilamide, and benzene-1,3-disulfonamide, with KIs in the range of 54\u201395 nM. CAS3 generally shows a higher affinity for this class of inhibitors compared to CAS1 and CAS2. As S. macrospora is a model organism for the study of fruiting body development in fungi, these data may be useful for developing antifungal compounds based on CA inhibition.<\/jats:p>","DOI":"10.3390\/molecules25051036","type":"journal-article","created":{"date-parts":[[2020,2,27]],"date-time":"2020-02-27T03:21:16Z","timestamp":1582773676000},"page":"1036","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sulfonamide Inhibition Studies of the \u03b2-Class Carbonic Anhydrase CAS3 from the Filamentous Ascomycete Sordaria macrospora"],"prefix":"10.3390","volume":"25","author":[{"given":"Daniela","family":"Vullo","sequence":"first","affiliation":[{"name":"Dipartimento di Chimica Ugo Schiff, Universit\u00e0 degli Studi di Firenze, 50019 Sesto Fiorentino (Florence), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronny","family":"Lehneck","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August-University, 37077 Gottingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6622-8193","authenticated-orcid":false,"given":"William A.","family":"Donald","sequence":"additional","affiliation":[{"name":"University of New South Wales, School of Chemistry, Sydney, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6842-4489","authenticated-orcid":false,"given":"Stefanie","family":"P\u00f6ggeler","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August-University, 37077 Gottingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"University of New South Wales, School of Chemistry, Sydney, NSW 2052, Australia"},{"name":"Neurofarba Dept., Section of Pharmaceutical and Nutriceutical Sciences, Universit\u00e0 degli Studi di Firenze, 50019 Sesto Fiorentino (Florence), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,25]]},"reference":[{"key":"ref_1","first-page":"201","article-title":"The filamentous fungus Sordaria macrospora as a genetic model to study fruiting body development","volume":"87","author":"Teichert","year":"2014","journal-title":"Adv. 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