{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:50:42Z","timestamp":1782391842095,"version":"3.54.5"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u0420\u043e\u0441\u0441\u0438\u0439\u0441\u043a\u0438\u0439 \u0424\u043e\u043d\u0434 \u0424\u0443\u043d\u0434\u0430\u043c\u0435\u043d\u0442\u0430\u043b\u044c\u043d\u044b\u0445 \u0418\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u0439 (\u0420\u0424\u0424\u0418)","award":["19-03-00043"],"award-info":[{"award-number":["19-03-00043"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>Enzyme-catalyzed hydrolysis of echothiophate, a P\u2013S bonded organophosphorus (OP) model, was spectrofluorimetrically monitored, using Calbiochem Probe IV as the thiol reagent. OP hydrolases were: the G117H mutant of human butyrylcholinesterase capable of hydrolyzing OPs, and a multiple mutant of Brevundimonas diminuta phosphotriesterase, GG1, designed to hydrolyze a large spectrum of OPs at high rate, including V agents. Molecular modeling of interaction between Probe IV and OP hydrolases (G117H butyrylcholinesterase, GG1, wild types of Brevundimonas diminuta and Sulfolobus solfataricus phosphotriesterases, and human paraoxonase-1) was performed. The high sensitivity of the method allowed steady-state kinetic analysis of echothiophate hydrolysis by highly purified G117H butyrylcholinesterase concentration as low as 0.85 nM. Hydrolysis was michaelian with Km = 0.20 \u00b1 0.03 mM and kcat = 5.4 \u00b1 1.6 min\u22121. The GG1 phosphotriesterase hydrolyzed echothiophate with a high efficiency (Km = 2.6 \u00b1 0.2 mM; kcat = 53400 min\u22121). With a kcat\/Km = (2.6 \u00b1 1.6) \u00d7 107 M\u22121min\u22121, GG1 fulfills the required condition of potential catalytic bioscavengers. quantum mechanics\/molecular mechanics (QM\/MM) and molecular docking indicate that Probe IV does not interact significantly with the selected phosphotriesterases. Moreover, results on G117H mutant show that Probe IV does not inhibit butyrylcholinesterase. Therefore, Probe IV can be recommended for monitoring hydrolysis of P\u2013S bonded OPs by thiol-free OP hydrolases.<\/jats:p>","DOI":"10.3390\/molecules25061371","type":"journal-article","created":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T07:29:07Z","timestamp":1584689347000},"page":"1371","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P\u2013S Bonded Organophosphorus as Monitored by Spectrofluorimetry"],"prefix":"10.3390","volume":"25","author":[{"given":"Irina V.","family":"Zueva","sequence":"first","affiliation":[{"name":"Arbuzov Institute of Organic and Physical Chemistry, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, Arbuzov str. 8, 420088 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6922-020X","authenticated-orcid":false,"given":"Sofya V.","family":"Lushchekina","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygin str 4, 119334 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Daud\u00e9","sequence":"additional","affiliation":[{"name":"Gene&amp;GreenTK, HU M\u00e9diterran\u00e9e Infection, Jean Moulin Blvd 19\u201321, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Chabri\u00e8re","sequence":"additional","affiliation":[{"name":"Aix-Marseille University, IRD, APHM, MEPHI, IHU-M\u00e9diterran\u00e9e Infection, 15005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7837-3662","authenticated-orcid":false,"given":"Patrick","family":"Masson","sequence":"additional","affiliation":[{"name":"Kazan Federal University, Neuropharmacology Laboratory, Kremlevskaya str 18, 480002 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S0140-6736(07)61202-1","article-title":"Management of acute organophosphorus pesticide poisoning","volume":"371","author":"Eddleston","year":"2008","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.cca.2014.01.024","article-title":"Acute organophosphorus poisoning","volume":"431","author":"Chowdhary","year":"2014","journal-title":"Clin. 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