{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T22:28:40Z","timestamp":1773700120121,"version":"3.50.1"},"reference-count":19,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Zhejiang province of China","award":["LZ20C170004"],"award-info":[{"award-number":["LZ20C170004"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872363"],"award-info":[{"award-number":["31872363"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lactoferrin (Lf) is a conserved iron-binding glycoprotein with antimicrobial activity, which is present in secretions that recover mucosal sites regarded as portals of invaded pathogens. Although numerous studies have focused on exogenous Lf, little is known about its expression of endogenous Lf upon bacterial infection. In this study, we investigated the distribution of Lf in mice intestine during Escherichia coli (E. coli) K88 infection. PCR and immunohistology staining showed that mRNA levels of Lf significantly increased in duodenum, ileum and colon, but extremely decreased in jejunum at 8 h and 24 h after infection. Meanwhile, endogenous Lf was mostly located in the lamina propria of intestine villi, while Lf receptor (LfR) was in the crypts. It suggested that endogenous Lf-LfR interaction might not be implicated in the antibacterial process. In addition, it was interesting to find that the infiltration of neutrophils into intestine tissues was changed similarly to Lf expression. It indicated that the variations of Lf expression were rather due to an equilibrium between the recruitment of neutrophils and degranulation of activated neutrophils. Thus, this new knowledge will pave the way to a more effective understanding of the role of Lf in intestinal mucosal immunity.<\/jats:p>","DOI":"10.3390\/molecules25071496","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T05:55:16Z","timestamp":1585893316000},"page":"1496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Distribution of Lactoferrin Is Related with Dynamics of Neutrophils in Bacterial Infected Mice Intestine"],"prefix":"10.3390","volume":"25","author":[{"given":"Li","family":"Liang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen-Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-You","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Xia","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Social Medicine, School of Medicine, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-Hua","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1139\/o11-071","article-title":"A structural perspective on lactoferrin function","volume":"90","author":"Baker","year":"2012","journal-title":"Biochem. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1139\/o2012-018","article-title":"Lactoferrin--50 years on","volume":"90","author":"Brock","year":"2012","journal-title":"Biochem. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1139\/o2012-013","article-title":"Lactoferrin: An alternative view of its role in human biological fluids","volume":"90","author":"Alexander","year":"2012","journal-title":"Biochem. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.biochi.2008.05.015","article-title":"Antimicrobial properties of lactoferrin","volume":"91","author":"Jenssen","year":"2009","journal-title":"Biochimie"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1016\/j.jpeds.2016.02.071","article-title":"Overview of lactoferrin as a natural immune modulator","volume":"173","author":"Legrand","year":"2016","journal-title":"J. Pediatr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.jnutbio.2013.10.012","article-title":"Bioactive peptides derived from human milk proteins--mechanisms of action","volume":"25","author":"Wada","year":"2014","journal-title":"J. Nutr. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Drago-Serrano, M.E., Campos-Rodr\u00edguez, R., Carrero, J.C., and de la Garza, M. (2017). Lactoferrin: Balancing ups and downs of inflammation due to microbial infections. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18030501"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.3168\/jds.S0022-0302(05)72883-6","article-title":"Developmental gene expression of lactoferrin and effect of dietary iron on gene regulation of lactoferrin in mouse mammary gland","volume":"88","author":"Wang","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1046\/j.1523-1755.2000.00050.x","article-title":"Expression of lactoferrin in the kidney: Implications for innate immunity and iron metabolism","volume":"57","author":"Larsson","year":"2000","journal-title":"Kidney Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.repbio.2013.10.005","article-title":"Lactoferrin expression and secretion in the stallion epididymis","volume":"14","author":"Pearl","year":"2014","journal-title":"Reprod. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1139\/o11-056","article-title":"Lactoferrin, a key molecule in immune and inflammatory processes","volume":"90","author":"Legrand","year":"2012","journal-title":"Biochem. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1093\/clinids\/12.Supplement_1.S73","article-title":"Epidemiology of travelers\u2019 diarrhea and relative importance of various pathogens","volume":"1","author":"Black","year":"1990","journal-title":"Rev. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ahmed, I., Roy, B., Khan, S., Septer, S., and Umar, S. (2016). Microbiome, metabolome and inflammatory bowel disease. Microorganisms, 4.","DOI":"10.3390\/microorganisms4020020"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"988","DOI":"10.3945\/jn.114.192120","article-title":"Dietary Dietary arginine supplementation of mice alters the microbial population and activates intestinal innate immunity","volume":"144","author":"Ren","year":"2014","journal-title":"J. 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Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1089\/ars.2015.6458","article-title":"Lactoferrin, a pleiotropic protein in health and disease","volume":"24","author":"Mayeur","year":"2016","journal-title":"Antioxid. Redox Signal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1038\/mi.2012.24","article-title":"The role of neutrophils during intestinal inflammation","volume":"5","author":"Fournier","year":"2012","journal-title":"Mucosal. 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