{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:53:12Z","timestamp":1767649992139,"version":"build-2065373602"},"reference-count":24,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2011-0001759, 2014R1A1A1007464, and 2016R1D1A1A02937214"],"award-info":[{"award-number":["NRF-2011-0001759, 2014R1A1A1007464, and 2016R1D1A1A02937214"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["KK-1806-01"],"award-info":[{"award-number":["KK-1806-01"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Mycobacterium abscessus is the most difficult-to-treat nontuberculous mycobacteria because of its resistance to many antibiotics. In this study, we screened the Korea Chemical Bank library for a bioluminescent reporter assay to identify molecules capable of acting against M. abscessus. On application of the assay, rifamycin O showed excellent in vitro activity with a narrow range of the minimum inhibitory concentration required to inhibit the growth of 90% of the bacterium (MIC90 = 4.0\u20136.2 \u03bcM); its in vivo efficacy in the zebrafish (Danio rerio) infection model was comparable to that of rifabutin at 25 \u03bcM. Furthermore, rifamycin O did not show significant toxicity in cells and the zebrafish model. These results are the first in vivo indication that rifamycin O may be a drug candidate for treating M. abscessus infections.<\/jats:p>","DOI":"10.3390\/molecules25071597","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T05:55:16Z","timestamp":1585893316000},"page":"1597","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Rifamycin O, An Alternative Anti-Mycobacterium abscessus Agent"],"prefix":"10.3390","volume":"25","author":[{"given":"Bui Thi Bich","family":"Hanh","sequence":"first","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea"},{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"June-Woo","family":"Park","sequence":"additional","affiliation":[{"name":"Environmental Biology Research Group, Korea Institute of Toxicology, Jinju 52834, Korea"},{"name":"Human and Environmental Toxicology Program, Korea University of Science and Technology (UST), Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea"},{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bionano Technology, Hanyang University, Seoul 04763, Korea"},{"name":"Department of Molecular &amp; Life Science, Hanyang University, Ansan 15588, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4918-961X","authenticated-orcid":false,"given":"Chul-Su","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Bionano Technology, Hanyang University, Seoul 04763, Korea"},{"name":"Department of Molecular &amp; Life Science, Hanyang University, Ansan 15588, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiseok","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hanyang University College of Medicine, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinsheng","family":"Cui","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Chan","family":"Oh","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jichan","family":"Jang","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea"},{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.3201\/2109.141634","article-title":"Mycobacterium abscessus complex infections in humans","volume":"21","author":"Lee","year":"2015","journal-title":"Emerg. 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