{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:03:32Z","timestamp":1780632212371,"version":"3.54.1"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004166","name":"Warszawski Uniwersytet Medyczny","doi-asserted-by":"publisher","award":["mini-grant No. FW25\/NM3\/18"],"award-info":[{"award-number":["mini-grant No. FW25\/NM3\/18"]}],"id":[{"id":"10.13039\/501100004166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["Operational Programme \u2018Innovative economy\u2019 for 2007\u20132013"],"award-info":[{"award-number":["Operational Programme \u2018Innovative economy\u2019 for 2007\u20132013"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Preparations from the flowers or herb of the white dead nettle (Lamium album L.) are recommended for the treatment of upper respiratory tract disorders or as a topical medication for mild inflammation of the throat, mouth, and skin. Taking into consideration the significance of L. album in traditional medicines across Europe, as well as the lack of studies describing the quantities of their most abundant constituents, we aimed to design a high-performance liquid chromatography coupled with diode-array detection (HPLC\u2013DAD) method for potential standardization procedures of extracts from flowers of L. album. The HPLC\u2013DAD method was developed and validated for quantification of iridoids (lamalbid), phenolic acids\/depsides (chlorogenic acid), phenylpropanoids (verbascoside), and flavonoids (rutin; quercetin malonylhexoside; tiliroside) in aqueous and ethanolic-aqueous extracts of Lamii albi flos. The method was specific, accurate, and precise. Lamalbid was the most abundant compound both in aqueous (39.09 \u00b1 1.02 m\/g dry weight) and ethanolic-aqueous (26.66 \u00b1 0.64 m\/g dry weight) extracts. The quantities of selected compounds, except for chlorogenic acid and tiliroside, were higher in the aqueous extract than in the ethanolic-aqueous one. In conclusion, the method developed allowed for quantitation of compounds from different classes. In particular, chlorogenic acid and verbascoside have been proposed as reference compounds for routine quantitative control of Lamii albi flos.<\/jats:p>","DOI":"10.3390\/molecules25071721","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T05:31:16Z","timestamp":1586496676000},"page":"1721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Lamalbid, Chlorogenic Acid, and Verbascoside as Tools for Standardization of Lamium album Flowers\u2014Development and Validation of HPLC\u2013DAD Method"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-0174","authenticated-orcid":false,"given":"Monika E.","family":"Czerwi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banach street, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Kalinowska","sequence":"additional","affiliation":[{"name":"Students\u2019 Scientific Association at the Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banach street, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4514-4193","authenticated-orcid":false,"given":"Dominik","family":"Popowski","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banach street, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9630-8787","authenticated-orcid":false,"given":"Agnieszka","family":"Bazylko","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banach street, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,9]]},"reference":[{"key":"ref_1","unstructured":"(1998). Quality Control Methods for Medicinal Plant Materials, World Health Organization."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"596","DOI":"10.3724\/SP.J.1009.2013.00596","article-title":"Quality control of traditional Chinese medicines: A review","volume":"11","author":"Song","year":"2013","journal-title":"Chin. J. Nat. Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Tzima, K., Brunton, N.P., and Rai, D.K. (2018). Qualitative and quantitative analysis of polyphenols in Lamiaceae plants\u2014A review. Plants, 7.","DOI":"10.3390\/plants7020025"},{"key":"ref_4","unstructured":"ICH (2020, April 07). Harmonised Tripartite Guideline. Q2(R1) Validation of Analytical Procedures: Text and Methodology. Available online: https:\/\/pacificbiolabs.com\/wp-content\/uploads\/2017\/12\/Q2_R1__Guideline-4.pdf."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s11101-014-9349-1","article-title":"Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae)","volume":"13","author":"Zielinska","year":"2014","journal-title":"Phytochem. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.indcrop.2017.01.009","article-title":"Ontogenetic and trans-generational variation of essential oil composition in Agastache rugosa","volume":"97","author":"Zielinska","year":"2017","journal-title":"Ind. Crops Prod."},{"key":"ref_7","unstructured":"(2017). European Pharmacopoeia (Ph. Eur.), Council of Europe. [9th ed.]."},{"key":"ref_8","unstructured":"Bruneton, J. (1999). Pharmacognosy: Phytochemistry of Medicinal Plants, Lavoisier."},{"key":"ref_9","first-page":"43","article-title":"Ethnobotany, pharmacology and phytochemistry of the genus Lamium (Lamiaceae)","volume":"31","author":"Kaya","year":"2006","journal-title":"Fabad J. Pharm. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Salehi, B., Armstrong, L., Rescigno, A., Yeskaliyeva, B., Seitimova, G., Beyatli, A., Sharmeen, J., Mahomoodally, M.F., Sharopov, F., and Durazzo, A. (2019). Lamium plants-a comprehensive review on health benefits and biological activities. Molecules, 24.","DOI":"10.3390\/molecules24101913"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.3831\/KPI.2019.22.033","article-title":"Urtica dioica and Lamium album decrease glycogen synthase kinase-3 beta and increase K-Ras in diabetic rats","volume":"22","author":"Abedinzade","year":"2019","journal-title":"J. Pharmacopunct."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.3831\/KPI.2018.21.008","article-title":"Effects of standardized extracts of Lamium album and Urtica dioica on rat tracheal smooth muscle contraction","volume":"21","author":"Arefani","year":"2018","journal-title":"J. Pharmacopunct."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"37","DOI":"10.15171\/PS.2019.6","article-title":"The effects of Urtica dioica and Lamium album extracts on the expression level of cyclooxygenase-2 and caspase-3 in the liver and kidney of streptozotocin-induced diabetic rat","volume":"25","author":"Khanaki","year":"2019","journal-title":"Pharm. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"443","DOI":"10.2478\/acph-2019-0026","article-title":"Haemostatic activity of butanolic extracts of Lamium album and Lamium purpureum aerial parts","volume":"69","author":"Bubueanu","year":"2019","journal-title":"Acta Pharm."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/0031-9422(94)00727-B","article-title":"Phenylpropanoid esters from Lamium album flowers","volume":"38","author":"Budzianowski","year":"1995","journal-title":"Phytochemistry"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Czerwi\u0144ska, M.E., \u015awierczewska, A., and Granica, S. (2018). Bioactive constituents of Lamium album L. as inhibitors of cytokines secretion in human neutrophils. Molecules, 23.","DOI":"10.3390\/molecules23112770"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1055\/s-0043-107031","article-title":"Bioassay-guided isolation of iridoids and phenylpropanoids from aerial parts of Lamium album and their anti-inflammatory activity in human neutrophils","volume":"83","author":"Kiss","year":"2017","journal-title":"Planta Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.bse.2006.07.004","article-title":"LC-ESI-MS analysis of iridoid glucosides in Lamium species","volume":"35","author":"Alipieva","year":"2007","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.bse.2005.04.002","article-title":"Iridoid glucosides from Lamium album and Lamium maculatum (Lamiaceae)","volume":"34","author":"Alipieva","year":"2006","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.fitote.2006.04.004","article-title":"Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae","volume":"77","author":"Matkowski","year":"2006","journal-title":"Fitoterapia"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.foodres.2012.04.009","article-title":"Phenolic constituents of Lamium album: Focus on isoscutellarein derivatives","volume":"48","author":"Pereira","year":"2012","journal-title":"Food Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/13102818.2014.989094","article-title":"Changes in the functional characteristics of tumor and normal cells after treatment with extracts of white dead-nettle","volume":"29","author":"Veleva","year":"2015","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1016\/j.jplph.2014.05.010","article-title":"Adaptive changes in photosynthetic performance and secondary metabolites during white dead nettle micropropagation","volume":"171","author":"Dimitrova","year":"2014","journal-title":"J. Plant Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3530","DOI":"10.1002\/jsfa.8207","article-title":"Irrigation and fruit canopy position modify oil quality of olive trees (cv. Frantoio)","volume":"97","author":"Caruso","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/pca.2558","article-title":"Quantitative determination of secoiridoids and phenylpropanoids in different extracts of Ligustrum vulgare L. leaves by a validated HPTLC\u2013photodensitometry method","volume":"26","author":"Czerwinska","year":"2015","journal-title":"Phytochem. Anal."},{"key":"ref_26","first-page":"202","article-title":"Seasonal variations in antioxidant compounds of Olea europaea leaves collected from different Italian cultivars","volume":"89","author":"Blasi","year":"2016","journal-title":"J. App. Bot. Food Qual."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.foodres.2018.11.046","article-title":"Impact of conventional\/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves","volume":"115","author":"Rocchetti","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1080\/13880200390502559","article-title":"Separation and quantification of tiliroside from plant extracts by SPE\/RP-HPLC","volume":"41","author":"Nowak","year":"2003","journal-title":"Pharm. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/S0305-1978(01)00035-7","article-title":"Phytoecdysteroids from Lamium spp: Identification and distribution within plants","volume":"29","author":"Savchenko","year":"2001","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S0305-1978(02)00165-5","article-title":"Phenylpropanoid glycosides and diterpenoids from Salvia officinalis","volume":"31","author":"Hohmann","year":"2003","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/j.foodchem.2007.05.016","article-title":"Antioxidative properties and phytochemical composition of Ballota nigra infusion","volume":"105","author":"Sousa","year":"2007","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1007\/s00044-017-1817-x","article-title":"Iridoid and phenylpropanoid glycosides from the roots of Lantana montevidensis","volume":"26","author":"Mohamed","year":"2017","journal-title":"Med. Chem. Res."},{"key":"ref_33","unstructured":"(2016). Deutscher Arzneimittel-Codex\/Neus Rezeptur Formularium (DAC\/NRF). Monographie T-017. Taubnesselkraut (Lamii albi herba), ABDA\u2014Bundesvereiningung Deutscher Apothekerverb\u04d3nde. Govi-Verlag Pharmazeutischer Verlag GmbH. Eschborn Deutscher Apotheker Verlag. [5th ed.]."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tutin, T., Heywood, V., Burges, N., Moore, D., Valentine, D., Walters, S., and Webb, D. (1972). Flora Europaea, Cambridge University Press.","DOI":"10.2307\/1218149"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/7\/1721\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:16:47Z","timestamp":1760174207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/7\/1721"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,9]]},"references-count":34,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["molecules25071721"],"URL":"https:\/\/doi.org\/10.3390\/molecules25071721","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,9]]}}}