{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:33:15Z","timestamp":1789155195887,"version":"build-2803163510"},"reference-count":21,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Scientific and Technical Research Council","award":["PIP 11220110101153"],"award-info":[{"award-number":["PIP 11220110101153"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The treatment of leishmaniasis includes pentavalent antimony drugs but, because of the side effects, toxicity and cases of treatment failure or resistance, the search of new antileishmanial compounds are necessary. The aims of this study were to evaluate and compare the in vitro antileishmanial activity of four green tea catechins, and to assess the efficacy of topical (\u2212)-epigallocatechin gallate in a cutaneous leishmaniasis model. The antileishmanial activity of green tea catechins was evaluated against intracellular amastigotes, and cytotoxicity was performed with human monocytic cell line. BALB\/c mice were infected in the ear dermis with Leishmania (Leishmania) amazonensis and treated with topical 15% (\u2212)-epigallocatechin gallate, intraperitoneal Glucantime, and control group. The efficacy of treatments was evaluated by quantifying the parasite burden and by measuring the lesions size. (\u2212)-Epigallocatechin gallate and (\u2212)-epigallocatechin were the most active compounds with IC50 values &lt;59.6 \u00b5g\/mL and with a selectivity index &gt;1. Topical treatment with (\u2212)-epigallocatechin gallate decreased significantly both lesion size and parasite burden (80.4% inhibition) compared to control group (p &lt; 0.05), and moreover (\u2212)-epigallocatechin gallate showed a similar efficacy to Glucantime (85.1% inhibition), the reference drug for leishmaniasis treatment.<\/jats:p>","DOI":"10.3390\/molecules25071741","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T03:10:01Z","timestamp":1586833801000},"page":"1741","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Efficacy of Topical Treatment with (\u2212)-Epigallocatechin Gallate, A Green Tea Catechin, in Mice with Cutaneous Leishmaniasis"],"prefix":"10.3390","volume":"25","author":[{"given":"Andrea M.","family":"Sosa","sequence":"first","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta-CONICET, Salta 4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9125-8124","authenticated-orcid":false,"given":"Agust\u00edn","family":"Moya \u00c1lvarez","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta-CONICET, Salta 4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Bracamonte","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta-CONICET, Salta 4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Korenaga","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Kochi Medical School, Kochi University, Okocho Kohasu, Nankoku, Kochi Prefecture 783-8505, Japan"},{"name":"Faculty of Health Sciences, Kochi Gakuen University, Asahi-Tenjincho, Kochi, Kochi Prefecture 780-0955, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge D.","family":"Marco","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta-CONICET, Salta 4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola A.","family":"Barroso","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta-CONICET, Salta 4400, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0140-6736(18)31204-2","article-title":"Leishmaniasis","volume":"15","author":"Burza","year":"2018","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.4269\/ajtmh.2001.64.187","article-title":"Treatment failure in children in a randomized clinical trial with 10 and 20 days of meglumine antimonate for cutaneous leishmaniasis due to Leishmania viannia species","volume":"64","author":"Palacios","year":"2001","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/0006-2952(87)90724-6","article-title":"Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiluminescence","volume":"36","author":"Fraga","year":"1987","journal-title":"Biochem. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0378-8741(95)90039-X","article-title":"Antibacterial effect of theaflavin, polyphenon 60 (Camellia sinensis) and Euphorbia hirta on Shigella spp.\u2014A cell culture study","volume":"49","author":"Vijaya","year":"1995","journal-title":"J. Ethnopharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1158\/1078-0432.CCR-04-1976","article-title":"Growth inhibitory and antimetastatic effect of green tea polyphenols on metastasis-specific mouse mammary carcinoma 4T1 cells in vitro and in vivo systems","volume":"11","author":"Manjeshwar","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.exppara.2007.04.015","article-title":"Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)","volume":"117","author":"Esteva","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/AAC.48.1.69-74.2004","article-title":"Anti-Trypanosoma cruzi activity of green tea (Camellia sinensis) catechins","volume":"48","author":"Paveto","year":"2004","journal-title":"Antimicrob. Agents. Chemother."},{"key":"ref_8","first-page":"104","article-title":"Antileishmanial activity of green tea (Camellia sinensis) catechins against Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis. Studies on New and Old World Leishmaniasis and their transmission, with particular reference to Ecuador, Peru, Argentina and Pakistan","volume":"8","author":"Barroso","year":"2007","journal-title":"Res. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1021\/np400624d","article-title":"In vitro and in vivo effects of (\u2212)-epigallocatechin 3-O-gallate on Leishmania amazonensis","volume":"25","author":"Inacio","year":"2013","journal-title":"J. Nat. Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.exppara.2012.06.008","article-title":"Mitochondrial damage contribute to epigallocatechin-3-gallate induced death in Leishmania amazonensis","volume":"132","author":"Inacio","year":"2012","journal-title":"Exp. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Inacio, J.D., Gervazoni, L., Canto-Cavalheiro, M.M., and Almeida-Amaral, E.E. (2014). The effect of (\u2212)-epigallocatechin 3-O-gallate in vitro and in vivo in Leishmania braziliensis: Involvement of reactive oxygen species as a mechanism of action. PLoS Negl. Trop. Dis., 21.","DOI":"10.1371\/journal.pntd.0003093"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Dos Reis, M.B., Manjolin, L.C., Maquiaveli, C.C., Santos-Filho, O.A., and da Silva, E.R. (2013). Inhibition of Leishmania (Leishmania) amazonensis and rat arginases by green tea EGCG, (+)-catechin and (\u2212)-epicatechin: A comparative structural analysis of enzyme-inhibitor interactions. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0078387"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1017\/S0031182017000385","article-title":"Safety and efficacy of current alternatives in the topical treatment of cutaneous leishmaniasis: A systematic review","volume":"144","author":"Navasconi","year":"2017","journal-title":"Parasitology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s002800050903","article-title":"Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin","volume":"43","author":"Dvorakova","year":"1999","journal-title":"Cancer Chemother. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/AAC.49.11.176-182.2005","article-title":"Antileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium","volume":"49","author":"Tiuman","year":"2005","journal-title":"Antimicrob Agents Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1128\/JCM.00216-12","article-title":"Novel approach to in vitro drug susceptibility assessment of clinical strains of Leishmania spp.","volume":"50","author":"Valderrama","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1128\/AAC.50.4.1352-1364.2006","article-title":"Antitrypanosomal and antileishmanial activities of flavonoids and their analogues: In vitro, in vivo, structure-activity relationship, and quantitative structure-activity relationship studies","volume":"50","author":"Tasdemir","year":"2006","journal-title":"Antimicrob Agents Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"606","DOI":"10.4269\/ajtmh.2005.72.606","article-title":"Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in North Argentina","volume":"72","author":"Marco","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1080\/20477724.2017.1312777","article-title":"In vitro antileishmanial activity of fisetin flavonoid via inhibition of glutathione biosynthesis and arginase activity in Leishmania infantum","volume":"111","author":"Adinehbeigi","year":"2017","journal-title":"Pathog. Glob. Health."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0169-4758(96)40010-2","article-title":"A simple method for quantifying Leishmania in tissues of infected animals","volume":"13","author":"Lima","year":"1997","journal-title":"Parasitol. Today."},{"key":"ref_21","unstructured":"Pretlow, T.G., and Pretlow, T.P. (1986). Limiting dilution assays for the separation, characterization and quantification of biologically active particles and their clonal progeny. Cell Separation: Methods and Selected Applications, Academic Press."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/7\/1741\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:17:16Z","timestamp":1760174236000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/7\/1741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,10]]},"references-count":21,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["molecules25071741"],"URL":"https:\/\/doi.org\/10.3390\/molecules25071741","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,10]]}}}