{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:40:08Z","timestamp":1790055608257,"version":"4.0.1"},"reference-count":59,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UBB of Cluj-Napoca (Romania) by Grant to support competitiveness","award":["Nr. AGC 30411 from 5.02.2020 (https:\/\/infocercetare.ubbcluj.ro\/Proiecte\/Proiecte\/Edit?id=14721), Nr. AGC 30412 from 5.02.2020 (https:\/\/infocercetare.ubbcluj.ro\/Proiecte\/Proiecte\/Edit?id=14722) and. Nr. AGC 30413 from 2020 (https:\/\/infocercetare.ubbcluj.ro"],"award-info":[{"award-number":["Nr. AGC 30411 from 5.02.2020 (https:\/\/infocercetare.ubbcluj.ro\/Proiecte\/Proiecte\/Edit?id=14721), Nr. AGC 30412 from 5.02.2020 (https:\/\/infocercetare.ubbcluj.ro\/Proiecte\/Proiecte\/Edit?id=14722) and. Nr. AGC 30413 from 2020 (https:\/\/infocercetare.ubbcluj.ro"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>5-fluorouracil (5-FU) is an anticancer drug used to inhibit the proliferation of many different tumor cells. Since severe events are associated with this compound, its combination with different anticancer drugs or adjuvants would allow the use of a significantly lower dose of 5-FU. In this study, we highlighted that the combination of allicin with 5-FU inhibited the cell migration and proliferation of colorectal and lung cancer cells. 5-FU inhibited cell growth with a similar inhibitory concentration for both normal and tumor cells (~200\u00b5M), while allicin showed different inhibitory concentrations. With an IC50 of 8.625 \u00b5M, lung cancer cells were the most sensitive to allicin. Compared to 5-FU and allicin single-agent treatments, the co-treatment showed a reduced viability rate, with p &lt; 0.05. The morphological changes were visible on all three cell lines, indicating that the treatment inhibited the proliferation of both normal and tumor cells. We highlighted different cell death mechanisms\u2014apoptosis for lung cancer and a non-apoptotic cell death for colorectal cancer. The synergistic antitumor effect of 5-FU combined with allicin was visible against lung and colorectal carcinoma cells. Better results were obtained when a lower concentration of 5-FU was combined with allicin than the single-agent treatment at IC50.<\/jats:p>","DOI":"10.3390\/molecules25081947","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T02:10:52Z","timestamp":1587607852000},"page":"1947","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-0791","authenticated-orcid":false,"given":"Adrian Bogdan","family":"\u021aigu","sequence":"first","affiliation":[{"name":"MedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400349 Cluj-Napoca, Romania"},{"name":"Faculty of Biology and Geology, Babe\u0219-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlad-Alexandru","family":"Toma","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Geology, Babe\u0219-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, Romania"},{"name":"Institute of Biological Research Cluj-Napoca, branch of NIRDBS Bucuresti, 400113 Cluj-Napoca, Romania"},{"name":"Department of Molecular and Biomolecular Physics, National Institute for R&D of Isotopic and MolecularTechnologies, 67-103 Donat, 400293 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustin C\u0103t\u0103lin","family":"Mo\u021b","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University,11 Arany Janos Street, 400028 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ancu\u021ba","family":"Jurj","sequence":"additional","affiliation":[{"name":"The Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400028 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-4622","authenticated-orcid":false,"given":"Cristian Silviu","family":"Moldovan","sequence":"additional","affiliation":[{"name":"MedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400349 Cluj-Napoca, Romania"},{"name":"The Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400028 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-0547","authenticated-orcid":false,"given":"Eva","family":"Fischer-Fodor","sequence":"additional","affiliation":[{"name":"MedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400349 Cluj-Napoca, Romania"},{"name":"Department of Radiobiology and Tumor Biology, the Oncology Institute \u201cProf Dr Ion Chiricuta\u201d, 400028 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3856-3784","authenticated-orcid":false,"given":"Ioana","family":"Berindan-Neagoe","sequence":"additional","affiliation":[{"name":"MedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, 400349 Cluj-Napoca, Romania"},{"name":"The Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400028 Cluj-Napoca, Romania"},{"name":"Department of Functional Genomics and Experimental Pathology, the Oncology Institute \u201cProf Dr Ion Chiricuta\u201d, 400028 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5892-9280","authenticated-orcid":false,"given":"Marcel","family":"P\u00e2rvu","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Geology, Babe\u0219-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tomeh, M.A., Hadianamrei, R., and Zhao, X. 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