{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:45:05Z","timestamp":1777923905697,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800255"],"award-info":[{"award-number":["31800255"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871694"],"award-info":[{"award-number":["31871694"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LQ16C020002"],"award-info":[{"award-number":["LQ16C020002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Salvia mltiorrhiza Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB root has not been verified so far. The purpose of this study was to establish a fast and simple method to quantify the tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh Salvia Miltiorrhiza Bunge root without any pre-purification steps using 1H-NMR spectroscopy. The process is as follows: first, 70% methanol aqueous extracts of fresh Salvia Miltiorrhiza Bunge roots were quantitatively analyzed for tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone using 1H-NMR spectroscopy. Different internal standards were tested and the validated method was compared with HPLC. 3,4,5-trichloropyridine was chosen as the internal standard. Twelve samples of Salvia Miltiorrhiza Bunge were quantitatively analyzed by qNMR and HPLC respectively. Then, the results were analyzed by chemometric approaches. This NMR method offers a fast, stable, and accurate analysis of four ketones: tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh roots of Salvia Miltiorrhiza Bunge.<\/jats:p>","DOI":"10.3390\/molecules25092043","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T01:24:18Z","timestamp":1588123458000},"page":"2043","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Quantitative Determination and Validation of Four Ketones in Salvia miltiorrhiza Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy"],"prefix":"10.3390","volume":"25","author":[{"given":"Yuanyuan","family":"Li","sequence":"first","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoni","family":"Hou","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-9830","authenticated-orcid":false,"given":"Feng","family":"Su","sequence":"additional","affiliation":[{"name":"Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 18 Chao Wang Road, Hangzhou 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jipeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 18 Chao Wang Road, Hangzhou 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodan","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongfeng","family":"Yang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongsuo","family":"Liang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.ymben.2011.02.003","article-title":"Metabolic engineering tanshinone biosynthetic pathway in Salvia miltiorrhiza hairy root cultures","volume":"13","author":"Kai","year":"2011","journal-title":"Metab. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s10142-014-0385-0","article-title":"Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase","volume":"14","author":"Shi","year":"2014","journal-title":"Funct. Integr. Genomics"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1080\/10408398.2018.1474170","article-title":"Bioactivities, biosynthesis and biotechnological production of phenolic acids in Salvia miltiorrhiza","volume":"59","author":"Shi","year":"2018","journal-title":"Crit. Rev. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00253-010-2797-7","article-title":"Tanshinone biosynthesis in Salvia miltiorrhiza and production in plant tissue cultures","volume":"88","author":"Wang","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e10554","DOI":"10.1038\/s41598-017-10215-2","article-title":"Comprehensive transcriptome profiling of Salvia miltiorrhiza for discovery of genes associated with the biosynthesis of tanshinone and salvianolic acids","volume":"7","author":"Zhou","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4172\/2529-797X.1000005","article-title":"Clinical research investigating salvianolate injection for treating coronary angina pectoris of Qi blood stagnation","volume":"1","author":"Zhao","year":"2015","journal-title":"Curr. Res. Integr. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e2767","DOI":"10.1038\/cddis.2017.174","article-title":"Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation","volume":"8","author":"Lu","year":"2017","journal-title":"Cell Death Dis."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cha, J.D., Lee, J.H., Choi, K.M., Choi, S.M., and Park, J.H. (2014). Synergistic Effect between cryptotanshinone and antibiotics against clinic methicillin and vancomycin-resistant Staphylococcus aureus. Evid-Based Compl. Alt.","DOI":"10.1155\/2014\/450572"},{"key":"ref_9","first-page":"1514","article-title":"Advances in pharmacological research and clinical application of tanshinone","volume":"26","author":"Wang","year":"2011","journal-title":"Bright Chin. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"422","DOI":"10.2174\/1389201020666190404125213","article-title":"Tanshinone IIA Promotes Macrophage Cholesterol Efflux and Attenuates Atherosclerosis of apoE-\/- Mice by Omentin-1\/ABCA1 Pathway","volume":"20","author":"Tan","year":"2019","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_11","first-page":"217","article-title":"Researches on tanshinone of Salvia miltiorrhiza\u2019s fat-soluble active ingredient","volume":"35","author":"Li","year":"2015","journal-title":"J. Hebei Univ. (Nat. Sci. Ed.)"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.1111\/jcmm.14539","article-title":"Tanshinone I inhibits the growth and metastasis of osteosarcoma via suppressing JAK\/STAT3 signalling pathway","volume":"23","author":"Wang","year":"2019","journal-title":"J. Cell. Mol. Med."},{"key":"ref_13","first-page":"2631","article-title":"Research progress on molecular mechanism of tumor cell apoptosis induced by cryptotanshinone","volume":"31","author":"Ge","year":"2013","journal-title":"Chin. Arch. Tradit. Chin. Med."},{"key":"ref_14","first-page":"1633","article-title":"Inhibitory effect of dihydrotanshinone on proliferation of colorectal cancer cells by regulating Wnt\/\u03b2-catenin signaling pathways","volume":"35","author":"Cheng","year":"2019","journal-title":"Chin. J. Clin. Pharm."},{"key":"ref_15","first-page":"443","article-title":"Protective effect and mechanism of tanshinone IIA on neuronal apoptosis after hypoxic-ischemic brain damage in neonatal rats","volume":"31","author":"Wang","year":"2015","journal-title":"Chin. Pharm. Bull."},{"key":"ref_16","first-page":"487","article-title":"The content of four tanshinones in danshen tablets was determined by HPLC","volume":"34","author":"Qin","year":"2019","journal-title":"Northwestern J. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liang, S., Wang, Z., Yuan, J., Zhang, J., Dai, X., Qin, F., Zhang, J., and Sun, Y. (2019). Rapid Identification of Tanshinone IIA Metabolites in an Amyloid-\u03b21-42 Induced Alzherimer\u2019s Disease Rat Model using UHPLC-Q-Exactive Qrbitrap Mass Spectrometry. Molecules, 24.","DOI":"10.3390\/molecules24142584"},{"key":"ref_18","first-page":"690","article-title":"Determination of cryptotanshinone and tanshinone \u2161 A in human plasma by LC-MS\/MS","volume":"35","author":"Zhang","year":"2019","journal-title":"Chin. J. Clin. Pharm."},{"key":"ref_19","unstructured":"Lan, T.F. (2011). Studies on the Tanshinone Components and Determination Method of Salvia miltiorrhiza. [Master\u2019s Thesis, Shandong University of Traditional Chinese Medicine]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/nprot.2009.237","article-title":"NMR-based metabolomic analysis of plants","volume":"5","author":"Kim","year":"2010","journal-title":"Nat. Protoc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.foodchem.2018.09.008","article-title":"1H NMR-based metabolic characterization of Chinese Wuding chicken meat","volume":"274","author":"Xiao","year":"2019","journal-title":"Food. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.talanta.2018.09.036","article-title":"A validated 1H qNMR method for direct and simultaneous quantification of esculin, fraxin and (\u2212)-epicatechin in Hippocastani cortex","volume":"192","author":"Owczarek","year":"2019","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/pca.1198","article-title":"HPLC fingerprints combined with principal component analysis, hierarchical cluster analysis and linear discriminant analysis for the classification and differentiation of Peganum sp. indigenous to China","volume":"3","author":"Cheng","year":"2010","journal-title":"Phytochem. Anal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1002\/pca.2571","article-title":"Cluster analysis of commercial samples of Bauhinia spp. using HPLC-UV\/PDA and MCR-ALS\/PCA without peak alignment procedure","volume":"5","author":"Ardila","year":"2015","journal-title":"Phytochem. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.talanta.2010.09.019","article-title":"GC\/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus","volume":"1","author":"Lin","year":"2010","journal-title":"Talanta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.talanta.2017.06.063","article-title":"Development of a HS-SPME-GC\/MS protocol assisted by chemometric tools to study herbivore-induced volatiles in Mycia splendens","volume":"175","author":"Silva","year":"2017","journal-title":"Talanta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.jpba.2005.01.043","article-title":"Validation of quantitative NMR","volume":"38","author":"Malz","year":"2005","journal-title":"J. Pharm. Biomed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1039\/c1ay05247k","article-title":"Quantitative determination and validation of Carvedilol in pharmaceuticals using quantitative nuclear magnetic resonance spectroscopy","volume":"3","author":"Gadape","year":"2011","journal-title":"Anal. Methods"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/9\/2043\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:23:58Z","timestamp":1760361838000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/9\/2043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,28]]},"references-count":28,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["molecules25092043"],"URL":"https:\/\/doi.org\/10.3390\/molecules25092043","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,28]]}}}