{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:06Z","timestamp":1787029086535,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:00:00Z","timestamp":1589241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romanian Ministry of Research and Innovation, CNCS\u2013UEFISCDI","award":["PN-III-P4-ID-PCCF-2016\u20130050"],"award-info":[{"award-number":["PN-III-P4-ID-PCCF-2016\u20130050"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Carbonic anhydrase (CA) is a zinc enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate and proton. Currently, CA inhibitors are widely used as antiglaucoma, anticancer, and anti-obesity drugs and for the treatment of neurological disorders. Recently, the potential use of CA inhibitors to fight infections caused by protozoa, fungi, and bacteria has emerged as a new research line. In this article, the X-ray crystal structure of \u03b2-CA from Burkholderia pseudomallei was reported. The X-ray crystal structure of this new enzyme was solved at 2.7 \u212b resolution, revealing a tetrameric type II \u03b2-CA with a \u201cclosed\u201d active site in which the zinc is tetrahedrally coordinated to Cys46, Asp48, His102, and Cys105. B. pseudomallei is known to encode at least two CAs, a \u03b2-CA, and a \u03b3-CA. These proteins, playing a pivotal role in its life cycle and pathogenicity, offer a novel therapeutic opportunity to obtain antibiotics with a different mechanism of action. Furthermore, the new structure can provide a clear view of the \u03b2-CA mechanism of action and the possibility to find selective inhibitors for this class of CAs.<\/jats:p>","DOI":"10.3390\/molecules25102269","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T10:53:55Z","timestamp":1589280835000},"page":"2269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Crystal Structure of a Tetrameric Type II \u03b2-Carbonic Anhydrase from the Pathogenic Bacterium Burkholderia pseudomallei"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1470-7192","authenticated-orcid":false,"given":"Andrea","family":"Angeli","sequence":"first","affiliation":[{"name":"NEUROFARBA Department, Sezione di Scienze Farmaceutiche, Via Ugo Schiff 6, Universit\u00e0 degli Studi di Firenze, 50019 Sesto Fiorentino (Florence), Italy"},{"name":"Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, \u201cPetru Poni\u201d Institute of Macromolecular Chemistry, 700487 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7258-738X","authenticated-orcid":false,"given":"Marta","family":"Ferraroni","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, Via della Lastruccia 13, Universit\u00e0 degli Studi di Firenze, I-50019 Sesto Fiorentino (Florence), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4021-982X","authenticated-orcid":false,"given":"Mariana","family":"Pinteala","sequence":"additional","affiliation":[{"name":"Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, \u201cPetru Poni\u201d Institute of Macromolecular Chemistry, 700487 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stelian S.","family":"Maier","sequence":"additional","affiliation":[{"name":"Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, \u201cPetru Poni\u201d Institute of Macromolecular Chemistry, 700487 Iasi, Romania"},{"name":"Polymers Research Center, Polymeric Release Systems Research Group, \u201cGheorghe Asachi\u201d Technical University of Iasi, 700487 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan C.","family":"Simionescu","sequence":"additional","affiliation":[{"name":"Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, \u201cPetru Poni\u201d Institute of Macromolecular Chemistry, 700487 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1141-6146","authenticated-orcid":false,"given":"Fabrizio","family":"Carta","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Sezione di Scienze Farmaceutiche, Via Ugo Schiff 6, Universit\u00e0 degli Studi di Firenze, 50019 Sesto Fiorentino (Florence), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Del Prete","sequence":"additional","affiliation":[{"name":"Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3314-2411","authenticated-orcid":false,"given":"Clemente","family":"Capasso","sequence":"additional","affiliation":[{"name":"Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Sezione di Scienze Farmaceutiche, Via Ugo Schiff 6, Universit\u00e0 degli Studi di Firenze, 50019 Sesto Fiorentino (Florence), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/bmb\/ldr007","article-title":"Melioidosis: A clinical overview","volume":"99","author":"Limmathurotsakul","year":"2011","journal-title":"Br. 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