{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:56:26Z","timestamp":1785333386420,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T00:00:00Z","timestamp":1589673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Silesia, Poland","award":["KNW-2-O14\/N\/9\/K, KNW-1-033\/N\/9\/O"],"award-info":[{"award-number":["KNW-2-O14\/N\/9\/K, KNW-1-033\/N\/9\/O"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The interaction of drugs with human serum albumin (HSA) is an important element of therapy. Albumin affects the distribution of the drug substance in the body, as well as its pharmacokinetic and pharmacodynamic properties. On the one hand, inflammation and protein glycation, directly associated with many pathological conditions and old age, can cause structural and functional modification of HSA, causing binding disorders. On the other hand, the widespread availability of various dietary supplements that affect the content of fatty acids in the body means that knowledge of the binding activity of transporting proteins, especially in people with chronic diseases, e.g., diabetes, will achieve satisfactory results of the selected therapy. Therefore, the aim of the present study was to evaluate the effect of a mixture of fatty acids (FA) with different saturated and unsaturated acids on the affinity of acetohexamide (AH), a drug with hypoglycaemic activity for glycated albumin, simulating the state of diabetes in the body. Based on fluorescence studies, we can conclude that the presence of both saturated and unsaturated FA disturbs the binding of AH to glycated albumin. Acetohexamide binds more strongly to defatted albumin than to albumin in the presence of fatty acids. The competitive binding of AH and FA to albumin may influence the concentration of free drug fraction and thus its therapeutic effect.<\/jats:p>","DOI":"10.3390\/molecules25102340","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:37:38Z","timestamp":1589971058000},"page":"2340","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["In Vitro Investigations of Acetohexamide Binding to Glycated Serum Albumin in the Presence of Fatty Acid"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6891-9625","authenticated-orcid":false,"given":"Agnieszka","family":"Szkudlarek","sequence":"first","affiliation":[{"name":"Department of Physical Pharmacy, Faculty of Pharmaceutical Siences in Sosnowiec, Medical University of Silesia in Katowice, 40-001 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5799-6337","authenticated-orcid":false,"given":"Micha\u0142","family":"Wilk","sequence":"additional","affiliation":[{"name":"Institute of Sport Sciences, Jerzy Kukuczka Academy of Physical Education in Katowice, 40-001 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-6309","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Maci\u0105\u017cek-Jurczyk","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy, Faculty of Pharmaceutical Siences in Sosnowiec, Medical University of Silesia in Katowice, 40-001 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.biochi.2010.12.003","article-title":"The glycation of albumin: Structural and functional impacts","volume":"93","author":"Rondeau","year":"2011","journal-title":"Biochimie"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1210\/jc.2007-1817","article-title":"The role of Advanced Glycation End Products in progression and complications of diabetes","volume":"93","author":"Goh","year":"2008","journal-title":"J. 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