{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T20:58:35Z","timestamp":1774731515291,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"High Value-Added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs","award":["117041-03-1-SB010"],"award-info":[{"award-number":["117041-03-1-SB010"]}]},{"name":"Ministry of Science and ICT grant","award":["2019A000022"],"award-info":[{"award-number":["2019A000022"]}]},{"name":"INNOPOLIS Foundation, Ministry of Science and ICT grant","award":["2019-DD-RD-0088"],"award-info":[{"award-number":["2019-DD-RD-0088"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Obesity is one of the most common metabolic diseases resulting in metabolic syndrome. In this study, we investigated the antiobesity effect of Gentiana lutea L. (GL) extract on 3T3-L1 preadipocytes and a high-fat-diet (HFD)-induced mouse model. For the induction of preadipocytes into adipocytes, 3T3-L1 cells were induced by treatment with 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone, and 1 \u03bcg\/mL insulin. Adipogenesis was assessed based on the messenger ribonucleic acid expression of adipogenic-inducing genes (adiponectin (Adipoq), CCAAT\/enhancer-binding protein alpha (Cebpa), and glucose transporter type 4 (Slc2a4)) and lipid accumulation in the differentiated adipocytes was visualized by Oil Red O staining. In vivo, obese mice were induced with HFD and coadministered with 100 or 200 mg\/kg\/day of GL extract for 12 weeks. GL extract treatment inhibited adipocyte differentiation by downregulating the expression of adipogenic-related genes in 3T3-L1 cells. In the obese mouse model, GL extract prevented HFD-induced weight gain, fatty hepatocyte deposition, and adipocyte size by decreasing the secretion of leptin and insulin. In conclusion, GL extract shows antiobesity effects in vitro and in vivo, suggesting that this extract can be beneficial in the prevention of obesity.<\/jats:p>","DOI":"10.3390\/molecules25102453","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T11:42:02Z","timestamp":1590406922000},"page":"2453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Antiobesity Effects of Gentiana lutea Extract on 3T3-L1 Preadipocytes and a High-Fat Diet-Induced Mouse Model"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1928-5531","authenticated-orcid":false,"given":"Eunkuk","family":"Park","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2031-0802","authenticated-orcid":false,"given":"Chang Gun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junho","family":"Kim","sequence":"additional","affiliation":[{"name":"Nine B Company, Daejeon 34121, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subin","family":"Yeo","sequence":"additional","affiliation":[{"name":"Nine B Company, Daejeon 34121, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Ae","family":"Kim","sequence":"additional","affiliation":[{"name":"Nine B Company, Daejeon 34121, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Whan","family":"Choi","sequence":"additional","affiliation":[{"name":"Natural Product Research Team, Biocenter, Gyeonggido Business and Science Accelerator, Suwon 16229, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0625-3530","authenticated-orcid":false,"given":"Seon-Yong","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Graduate School of Medicine, Ajou University, Suwon 16499, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/35007508","article-title":"Obesity as a medical problem","volume":"404","author":"Kopelman","year":"2000","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1038\/s41430-018-0100-7","article-title":"From fat cells through an obesity theory","volume":"72","author":"Sorensen","year":"2018","journal-title":"Eur. 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