{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T13:03:36Z","timestamp":1769605416193,"version":"3.49.0"},"reference-count":71,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T00:00:00Z","timestamp":1590883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Various diseases, including bacterial panicle blight (BPB) and sheath rot, threaten rice production. It has been established that Burkholderia glumae (B. glumae) is the causative agent of the above mentioned pathologies. In the present study, antagonistic activity, growth promotion, and the metabolite profiles of two rhizobacteria, isolated in different paddy fields, were assessed against B. glumae. Strains were identified based on 16S rRNA gene sequences, and the phylogenetic analyses showed that both strains belong to the genus Enterobacter, with high similarity to the strain Enterobacter tabaci NR146667.2 (99%). The antagonistic activity was assessed with the disc diffusion method. Active fractions were isolated through a liquid\/liquid extraction with ethyl acetate (EtOAc) from the fermentation media, and their antibacterial activities were evaluated following the Clinical and Laboratory Standards Institute (CLSI) guidelines. The Pikovskaya modified medium was used to test the ability of in vitro inorganic phosphorus solubilization, and BSB1 proved to be the best inorganic phosphorus solubilizer, with a solubilization index (SI) of 4.5 \u00b1 0.2. The glass-column fractionation of the EtOAc extracted from BCB11 produced an active fraction (25.9 mg) that inhibited the growth of five B. glumae strains by 85\u201395%. Further, metabolomic analysis, based on GC\u2013MS, showed 3-phenylpropanoic acid (3-PPA) to be the main compound both in this fraction (46.7%), and in the BSB1 extract (28.6%). This compound showed antibacterial activity against all five strains of B. glumae with a minimum inhibitory concentration (MIC) of 1000 mg\/L towards all of them. The results showed that rice rhizosphere microorganisms are a source of compounds that inhibit B. glumae growth and are promising plant growth promoters (PGP).<\/jats:p>","DOI":"10.3390\/molecules25112567","type":"journal-article","created":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T04:12:09Z","timestamp":1591157529000},"page":"2567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rice-Associated Rhizobacteria as a Source of Secondary Metabolites against Burkholderia glumae"],"prefix":"10.3390","volume":"25","author":[{"given":"Giann Carlos","family":"Pe\u00f1aloza Atuesta","sequence":"first","affiliation":[{"name":"Chemistry Department, Faculty of Sciences, University of Tolima, Ibagu\u00e9 730006299, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-070X","authenticated-orcid":false,"given":"Walter","family":"Murillo Arango","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Sciences, University of Tolima, Ibagu\u00e9 730006299, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8022-2540","authenticated-orcid":false,"given":"Jordi","family":"Eras","sequence":"additional","affiliation":[{"name":"Chemistry Department, Scientific Technical Services-TCEM, University of Lleida, 25198 Lleida, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0467-733X","authenticated-orcid":false,"given":"Diego Fern\u00e1ndo","family":"Oliveros","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Sciences, University of Tolima, Ibagu\u00e9 730006299, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonh Jairo","family":"M\u00e9ndez Arteaga","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Sciences, University of Tolima, Ibagu\u00e9 730006299, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.geoderma.2013.08.013","article-title":"The dimensions of soil security","volume":"213","author":"McBratney","year":"2014","journal-title":"Geoderma"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"495","DOI":"10.5958\/0976-4038.2014.00601.0","article-title":"Studies on yield, economics and energetics of rice (oryza sativa l.) in relation to crop establishment methods and nutrient management practices","volume":"5","author":"Mohanty","year":"2014","journal-title":"Int. J. Bio Resour. Stress Manag."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s12571-012-0168-1","article-title":"Crops that feed the world 7: Rice","volume":"4","author":"Seck","year":"2012","journal-title":"Food Secur."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Karki, H.S., Bishnu, K.S., Jae, W.H., Donald, E.G., Barphagha, I.K., Milton, C.R., Rebecca, A.M., Beom, S.K., and Jong, H.H. (2012). Diversities in virulence, antifungal activity, pigmentation and dna fingerprint among strains of burkholderia glumae. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0045376"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1007\/s00122-013-2145-2","article-title":"Identification of qrbs1, a qtl involved in resistance to bacterial seedling rot in rice","volume":"126","author":"Mizobuchi","year":"2013","journal-title":"Theor. Appl. Genet."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.rsci.2016.01.007","article-title":"Research status and prospect of burkholderia glumae, the pathogen causing bacterial panicle blight","volume":"23","author":"Cui","year":"2016","journal-title":"Rice Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Magbanua, Z.V., Mark, A., Teresia, B., Hsu, C.-Y., Showmaker, K.C., Philippe, C., Peng, D., Peterson, D.G., and Lu, S. (2014). Transcriptomic dissection of the rice\u2014Burkholderia glumae interaction. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-755"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jplph.2015.09.011","article-title":"Priming of pathogenesis related-proteins and enzymes related to oxidative stress by plant growth promoting rhizobacteria on rice plants upon abiotic and biotic stress challenge","volume":"188","author":"Ramos","year":"2015","journal-title":"J. Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1364-3703.2010.00676.x","article-title":"Burkholderia glumae: Next major pathogen of rice?","volume":"12","author":"Ham","year":"2011","journal-title":"Mol. Plant Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Shrestha, B.K., Hari, S.K., Donald, E.G., Nootjarin, J.K., and Jong, H.H. (2016). Biological control activities of rice-associated bacillus sp. strains against sheath blight and bacterial panicle blight of rice. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146764"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1186\/2193-1801-2-606","article-title":"Evaluation of rhizosphere, rhizoplane and phyllosphere bacteria and fungi isolated from rice in kenya for plant growth promoters","volume":"2","author":"Mwajita","year":"2013","journal-title":"SpringerPlus"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.pbi.2017.04.018","article-title":"Understanding and exploiting plant beneficial microbes","volume":"38","author":"Finkel","year":"2017","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.mib.2017.03.005","article-title":"Life in earth-the root microbiome to the rescue?","volume":"37","author":"Mauchline","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1093\/jxb\/erv157","article-title":"Role of root microbiota in plant productivity","volume":"66","author":"Tkacz","year":"2015","journal-title":"J. Exp. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.rhisph.2017.01.004","article-title":"Seed-Coating fenugreek with burkholderia rhizobacteria enhances yield in field trials and can combat fusarium wilt","volume":"3","author":"Kumar","year":"2017","journal-title":"Rhizosphere"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s12571-008-0002-y","article-title":"Food security: Definition and measurement","volume":"1","year":"2009","journal-title":"Food Secur."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1126\/science.1185383","article-title":"Food security: The challenge of feeding 9 billion people","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.tplants.2014.09.007","article-title":"What lies beneath: Belowground defense strategies in plants","volume":"20","author":"Barbara","year":"2015","journal-title":"Trends Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1007\/s00425-015-2382-5","article-title":"Differential responses of oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria","volume":"242","author":"Chamam","year":"2015","journal-title":"Planta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mib.2017.03.008","article-title":"The unseen rhizosphere root\u2013soil\u2013microbe interactions for crop production","volume":"37","author":"Zhang","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ecoleng.2015.07.019","article-title":"Using plant growth-promoting rhizobacteria (pgpr) to improve plant growth","volume":"84","author":"Grobelak","year":"2015","journal-title":"Ecol. Eng."},{"key":"ref_22","unstructured":"Harsh, B., and Janine, S. (2015). Chapter two-molecular patterns of rhizobacteria involved in plant immunity elicitation. Advances in Botanical Research, Academic Press."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.rhisph.2017.03.002","article-title":"Bacterial diversity of the rhizosphere and nearby surface soil of rice (oryza sativa) growing in the camargue (france)","volume":"3","author":"Osman","year":"2017","journal-title":"Rhizosphere"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"228","DOI":"10.5423\/PPJ.OA.10.2015.0218","article-title":"Biological control of rice bakanae by an endophytic bacillus oryzicola yc7007","volume":"32","author":"Hossain","year":"2016","journal-title":"Plant Pathol. J."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Su\u00e1rez-Moreno, Z.R., Vinchira-Villarraga, D.M., Vergara-Morales, D.I., Castellanos, L., Ramos, F.A., Guarnaccia, C., Degrassi, G., Venturi, V., and Moreno-Sarmiento, N. (2019). Plant-Growth promotion and biocontrol properties of three streptomyces spp. isolates to control bacterial rice pathogens. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00290"},{"key":"ref_26","first-page":"43","article-title":"Antifungal activity of pyocyanin produced by pseudomonas aeruginosa against fusarium oxysporum schlech a root-rot phytopathogenic fungi","volume":"9","author":"Mahmoud","year":"2016","journal-title":"IJPRIF"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1007\/s002849900037","article-title":"Pyrrolnitrin production by an enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens","volume":"32","author":"Leonid","year":"1996","journal-title":"Curr. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6968.2001.tb10556.x","article-title":"Enterobactin: The characteristic catecholate siderophore of enterobacteriaceae is produced by streptomyces species","volume":"196","author":"Krastel","year":"2001","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s003740050497","article-title":"Solubilization of inorganic phosphates and growth emergence of wheat as affected by azotobacter chroococcum mutants","volume":"28","author":"Kumar","year":"1999","journal-title":"Biol. Fertil. Soils"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"954","DOI":"10.3923\/ijar.2010.954.983","article-title":"Impact of plant growth promoting rhizobacteria on crop production","volume":"5","author":"Verma","year":"2010","journal-title":"Int. J. Agric. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00284-010-9674-6","article-title":"Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth","volume":"62","author":"Mathurot","year":"2011","journal-title":"Curr. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.ecoenv.2019.01.070","article-title":"Enhanced biodegradation of atrazine by arthrobacter sp. dns10 during co-culture with a phosphorus solubilizing bacteria: Enterobacter sp. p1","volume":"172","author":"Zhao","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1007\/s10482-015-0569-1","article-title":"Enterobacter tabaci sp. nov., a novel member of the genus enterobacter isolated from a tobacco stem","volume":"108","author":"Duan","year":"2015","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_34","first-page":"504","article-title":"Current overview on the study of bacteria in the rhizosphere by modern molecular techniques: A mini review","volume":"15","author":"Lagos","year":"2015","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_35","first-page":"117","article-title":"Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of polygonum cuspidatum and its endophytic actinomycete, streptomyces sp. A0916","volume":"14","author":"Wang","year":"2016","journal-title":"Chin. J. Nat. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.foodchem.2010.06.036","article-title":"Antityrosinase and antimicrobial activities of 2-phenylethanol, 2-phenylacetaldehyde and 2-phenylacetic acid","volume":"124","author":"Zhu","year":"2011","journal-title":"Food Chem."},{"key":"ref_37","first-page":"191","article-title":"Biological activity of phenylpropionic acid isolated from a terrestrial streptomycetes","volume":"56","author":"Narayana","year":"2007","journal-title":"Pol. J. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"19292","DOI":"10.3390\/molecules191219292","article-title":"Natural cinnamic acids, synthetic derivatives and hybrids with antimicrobial activity","volume":"19","author":"Guzman","year":"2014","journal-title":"Molecules"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.ejmech.2004.06.013","article-title":"Esters, amides and substituted derivatives of cinnamic acid: Synthesis, antimicrobial activity and qsar investigations","volume":"39","author":"Balasubramanian","year":"2004","journal-title":"Eur. J. Med. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.4067\/S0717-97072013000400024","article-title":"Biological activity of macromycetes isolated from chilean subantarctic ecosystems","volume":"58","author":"Rodrigo","year":"2013","journal-title":"J. Chil. Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5048","DOI":"10.1073\/pnas.0401416101","article-title":"Tyrosol is a quorum-sensing molecule in <em>candida albicans","volume":"101","author":"Chen","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1021\/jf0630217","article-title":"In vitro activity of olive oil polyphenols against helicobacter pylori","volume":"55","author":"Medina","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1016\/j.fct.2009.06.012","article-title":"enhanced bactericidal activity of enterocin as-48 in combination with essential oils, natural bioactive compounds and chemical preservatives against listeria monocytogenes in ready-to-eat salad","volume":"47","author":"Antonio","year":"2009","journal-title":"Food Chem. Toxicol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1365-2915.2007.00668.x","article-title":"Antibacterial substances of low molecular weight isolated from the blowfly, lucilia sericata","volume":"21","author":"Huberman","year":"2007","journal-title":"Med Vet. Entomol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.resmic.2010.04.006","article-title":"Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria","volume":"161","author":"Carolina","year":"2010","journal-title":"Res. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.ymben.2004.05.001","article-title":"Microbial export of lactic and 3-hydroxypropanoic acid: Implications for industrial fermentation processes","volume":"6","author":"Maris","year":"2004","journal-title":"Metab. Eng."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"629","DOI":"10.4315\/0362-028X-60.6.629","article-title":"Bactericidal activity of organic acids against salmonella typhimurium attached to broiler chicken skint","volume":"60","author":"Tamblyn","year":"1997","journal-title":"J. Food Prot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1039\/C8NP00032H","article-title":"Tryptophol and derivatives: Natural occurrence and applications to the synthesis of bioactive compounds","volume":"36","author":"Alessandro","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1007\/s11274-007-9392-9","article-title":"Tyrosol and tryptophol produced by ceratocystis adiposa","volume":"23","author":"Oswaldo","year":"2007","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.ejar.2016.10.006","article-title":"Induction of the synthesis of bioactive compounds of the marine alga tetraselmis tetrathele (west) butcher grown under salinity stress","volume":"42","year":"2016","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.phymed.2009.03.014","article-title":"Comparative research of chemical constituents, antifungal and antitumor properties of ether extracts of panax ginseng and its endophytic fungus","volume":"16","author":"Xu","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1093\/jxb\/erq412","article-title":"Auxin conjugates: Their role for plant development and in the evolution of land plants","volume":"62","author":"Jutta","year":"2011","journal-title":"J. Exp. Bot."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3795","DOI":"10.1128\/AEM.68.8.3795-3801.2002","article-title":"Role of pseudomonas putida indoleacetic acid in development of the host plant root system","volume":"68","author":"Patten","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jep.2005.05.038","article-title":"Antibacterial and antioxidant activities of ethanol extract from paullinia cupana mart","volume":"102","author":"Adriana","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00284-009-9501-0","article-title":"Antibacterial activity of phenolic compounds against the phytopathogen xylella fastidiosa","volume":"60","author":"Maddox","year":"2009","journal-title":"Curr. Microbiol."},{"key":"ref_56","first-page":"1235","article-title":"Anti-angiogenic, anti-inflammatory and anti-nociceptive activity of 4-hydroxybenzyl alcohol","volume":"59","author":"Jung","year":"2007","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.neures.2009.02.005","article-title":"Experimental stroke protection induced by 4-hydroxybenzyl alcohol is cancelled by bacitracin","volume":"64","author":"Elodie","year":"2009","journal-title":"Neurosci. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.brainres.2009.10.037","article-title":"Neuroprotective effect of 4-hydroxybenzyl alcohol against transient focal cerebral ischemia via anti-apoptosis in rats","volume":"1308","author":"Yu","year":"2010","journal-title":"Brain Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/jhet.349","article-title":"Biological importance of the indole nucleus in recent years: A comprehensive review","volume":"47","author":"Vikas","year":"2010","journal-title":"J. Heterocycl. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ijfoodmicro.2013.12.016","article-title":"Antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production","volume":"173","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1128\/AEM.67.8.3739-3745.2001","article-title":"Isolation and in vivo and in vitro antifungal activity of phenylacetic acid and sodium phenylacetate from streptomyces humidus","volume":"67","author":"Hwang","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s11947-008-0127-1","article-title":"Production and characterization of an antifungal compound (3-phenyllactic acid) produced by lactobacillus plantarum strain","volume":"3","author":"Prema","year":"2010","journal-title":"Food Bioprocess Technol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jbiotec.2016.11.016","article-title":"Biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass","volume":"257","author":"Lee","year":"2017","journal-title":"J. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Averesch, N.J.H., and Kr\u00f6mer, J.O. (2018). Metabolic engineering of the shikimate pathway for production of aromatics and derived compounds\u2014present and future strain construction strategies. Front. Bioeng. Biotechnol., 6.","DOI":"10.3389\/fbioe.2018.00032"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Wanger, A., Chavez, V., Huang, R.S.P., Wahed, A., Actor, J.K., and Dasgupta, A. (2017). Chapter 4-media for the clinical microbiology laboratory. Microbiology and Molecular Diagnosis in Pathology, Elsevier.","DOI":"10.1016\/B978-0-12-805351-5.00004-1"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.copbio.2009.09.012","article-title":"Production of aromatic compounds in bacteria","volume":"20","author":"Guillermo","year":"2009","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_67","first-page":"94","article-title":"Exploring the microbial production of aromatic fine chemicals to overcome the barriers of traditional methods","volume":"8","author":"Dias","year":"2017","journal-title":"Adv. Appl. Sci. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.rhisph.2018.06.002","article-title":"Metabolomic analysis of indolepyruvate decarboxylase pathway derivatives in the rhizobacterium enterobacter cloacae","volume":"6","author":"Harris","year":"2018","journal-title":"Rhizosphere"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1093\/nar\/21.9.2260","article-title":"A simple and rapid method for the preparation of gram-negative bacterial genomic DNA","volume":"21","author":"Chen","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"Mega X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_71","unstructured":"CLSI (2015). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard\u2014Tenth Edition, Clinical and Laboratory Standards Institute."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/11\/2567\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:34:22Z","timestamp":1760175262000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/11\/2567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,31]]},"references-count":71,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["molecules25112567"],"URL":"https:\/\/doi.org\/10.3390\/molecules25112567","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,31]]}}}