{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:57:28Z","timestamp":1787029048634,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004693","name":"Universiti Malaysia Kelantan","doi-asserted-by":"publisher","award":["R\/SGJP\/A0700\/00710A\/005\/2019\/00609"],"award-info":[{"award-number":["R\/SGJP\/A0700\/00710A\/005\/2019\/00609"]}],"id":[{"id":"10.13039\/501100004693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Alocasia longiloba, locally known as \u2018Keladi Candik\u2019, has been used traditionally to treat wounds, furuncle and joint inflammations. A. longiloba can be a new source of herbal medicine against hyperuricemia by inhibiting the activity of xanthine oxidase enzyme, the enzyme which is responsible for the development of hyperuricemia in human. Existing xanthine oxidase inhibitors (XOI drugs) show several side effects on gout patients. Therefore, an alternative herbal medicine from plants, with high therapeutic property and free of side effects, are greatly needed. This study was conducted to evaluate XO inhibitory activity, chemical composition, antioxidant activity and GC-MS profile of A. longiloba. Our results showed that ethanolic petiole extract exhibited the highest XO inhibitory activity (70.40 \u00b1 0.05%) with IC50 value of 42.71 \u03bcg\/mL, followed by ethanolic fruit extracts (61.44 \u00b1 1.24%) with the IC50 value of 51.32 \u03bcg\/mL. In a parallel study, the phytochemical analysis showed the presence of alkaloid, flavonoid, terpenoids, glycoside and saponin in petiole and fruit extracts, as well as higher total phenolic and flavonoid contents and strong scavenging activity on DPPH and ABTS antioxidant assay. The GC-MS analysis of fruit and petiole extracts revealed the presence of various compounds belonging to different chemical nature, among them are limonen-6-ol, \u03b1-DGlucopyranoside, paromomycin, aziridine, phenol, Heptatriacotanol, Phen-1,2,3-dimethyl and Betulin found in ethanolic fruit extract, and Phen-1,4-diol,2,3-dimethyl-, 1-Ethynyl-3,trans(1,1-dimethylethyl), Phenol,2,6-dimethoxy-4-(2-propenyl)- and 7-Methyl-Z-tetradecen-1-olacetate found in ethanolic petiole extract. Some compounds were documented as potent anti-inflammatory and arthritis related diseases by other researchers. In this study, the efficiency of solvents to extract bioactives was found to be ethanol &gt; water, methanol &gt; hexane &gt; chloroform. Together, our results suggest the prospective utilization of fruit and petiole of A. longiloba to inhibit the activity of XO enzyme.<\/jats:p>","DOI":"10.3390\/molecules25112658","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T08:49:11Z","timestamp":1591692551000},"page":"2658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Xanthine Oxidase Inhibitory Activity, Chemical Composition, Antioxidant Properties and GC-MS Analysis of Keladi Candik (Alocasia longiloba Miq)"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-6482","authenticated-orcid":false,"given":"Ferid","family":"Abdulhafiz","sequence":"first","affiliation":[{"name":"Faculty of Agro-Based Industry, University Malaysia Kelantan, Jeli Campus, Jeli 17600, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7793-8134","authenticated-orcid":false,"given":"Arifullah","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Faculty of Agro-Based Industry, University Malaysia Kelantan, Jeli Campus, Jeli 17600, Kelantan, Malaysia"},{"name":"Institute of Food Security and Sustainable Agriculture (IFSSA), University Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatimah","family":"Kayat","sequence":"additional","affiliation":[{"name":"Faculty of Agro-Based Industry, University Malaysia Kelantan, Jeli Campus, Jeli 17600, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matcha","family":"Bhaskar","sequence":"additional","affiliation":[{"name":"Division of Animal Biotechnology, Department of Zoology, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4837-4605","authenticated-orcid":false,"given":"Zulhazman","family":"Hamzah","sequence":"additional","affiliation":[{"name":"Faculty of Earth Science, University Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay Kumar","family":"Podapati","sequence":"additional","affiliation":[{"name":"Faculty of Agro-Based Industry, University Malaysia Kelantan, Jeli Campus, Jeli 17600, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8089-5219","authenticated-orcid":false,"given":"Lebaka Veeranjaneya","family":"Reddy","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh 516003, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"ref_1","unstructured":"Klemow, K.M., Bartlow, A., Crawford, J., Kocher, N., Shah, J., and Ritsick, M. (2011). Herbal Medicine: Biomolecular and Clinical Aspects, CRC Press."},{"key":"ref_2","first-page":"294858","article-title":"Xanthine oxidase: Isolation, assays of activity, and inhibition","volume":"8","author":"Kostic","year":"2015","journal-title":"J. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4103\/0253-7613.56063","article-title":"Medicinal plant-based health products: Where is the medicinal constituent?","volume":"41","author":"Mathur","year":"2009","journal-title":"Indian J. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Teke, G.N., and Kuete, V. (2014). Acute and subacute toxicities of African medicinal plants. Toxicological Survey of African Medicinal Plants, Elsevier.","DOI":"10.1016\/B978-0-12-800018-2.00005-4"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kabir, M.S.H., Hossain, M.M., Kabir, M.I., Ahmad, S., Chakrabarty, N., Rahman, M.A., and Rahman, M.M. (2016). Antioxidant, antidiarrheal, hypoglycemic and thrombolytic activities of organic and aqueous extracts of Hopea odorata leaves and in silico PASS prediction of its isolated compounds. BMC Complem. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1461-x"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.jare.2017.05.003","article-title":"Uric acid in plants and microorganisms: Biological applications and genetics\u2014A review","volume":"8","author":"Hafez","year":"2017","journal-title":"J. Adv. Res."},{"key":"ref_7","first-page":"16","article-title":"Prevalence of Hyperuricemia & relation of Serum uric Acid in Burn Patients amongst Different Gender and Age Groups","volume":"1","author":"Gaur","year":"2018","journal-title":"Int. J. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.1002\/elan.201500166","article-title":"Fabrication of a nanostructure based electrochemical sensor for voltammetric determination of epinephrine, uric acid and folic acid","volume":"27","author":"Beitollahi","year":"2015","journal-title":"Electroanalysis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.metabol.2017.06.006","article-title":"Uric acid promotes vascular stiffness, maladaptive inflammatory responses and proteinuria in western diet fed mice","volume":"74","author":"Aroor","year":"2017","journal-title":"Metabolism"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/1756-185X.13103","article-title":"Gout: An Asia-pacific update","volume":"20","author":"Paul","year":"2017","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0163-7258(81)90063-2","article-title":"Tight binding inhibitors of xanthine oxidase","volume":"14","author":"Hille","year":"1981","journal-title":"Pharmacol. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1021\/bi00363a004","article-title":"Noncompetitive and irreversible inhibition of xanthine oxidase by benzimidazole analogs acting at the functional flavin adenine dinucleotide cofactor","volume":"25","author":"Skibo","year":"1986","journal-title":"Biochemistry"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.ejmech.2017.11.071","article-title":"Xanthine oxidase inhibitory activity of natural and hemisynthetic flavonoids from Gardenia oudiepe (Rubiaceae) in vitro and molecular docking studies","volume":"143","author":"Santi","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.jep.2015.10.036","article-title":"Alocasia denudata Engler treatment enhance open wound healing activities in Wistar rat\u2019s skin","volume":"176","author":"Latif","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4103\/2221-1691.256727","article-title":"Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats","volume":"9","author":"Hamzah","year":"2019","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Jing, L., Ma, H., Fan, P., Gao, R., and Jia, Z. (2015). Antioxidant potential, total phenolic and total flavonoid contents of Rhododendron anthopogonoides and its protective effect on hypoxia-induced injury in PC12 cells. BMC Complem. Altern. Med., 15.","DOI":"10.1186\/s12906-015-0820-3"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"320","DOI":"10.4103\/0973-1296.90413","article-title":"Xanthine oxidase inhibitory activity of the methanolic extracts of selected Jordanian medicinal plants","volume":"7","author":"Hudaib","year":"2011","journal-title":"Pharmacogn. Mag."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"400","DOI":"10.3390\/molecules23020400","article-title":"The antioxidant and xanthine oxidase inhibitory activity of Plumeria rubra flowers","volume":"23","author":"Isa","year":"2018","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"C9","DOI":"10.1111\/j.1750-3841.2009.01392.x","article-title":"Phenolic composition and antioxidant activities of 11 celery cultivars","volume":"75","author":"Yao","year":"2010","journal-title":"J. Food Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.jfca.2010.12.005","article-title":"Total phenolic compounds and antioxidant potential of Hedychium spicatum Buch. Ham. ex D. Don in west Himalaya","volume":"24","author":"Rawat","year":"2011","journal-title":"India J. Food Compos. Anal."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 5.","DOI":"10.3390\/medicines5030093"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7320","DOI":"10.3390\/molecules16097320","article-title":"Toxicity of Rhododendron anthopogonoides essential oil and its constituent compounds towards Sitophilus zeamais","volume":"16","author":"Yang","year":"2011","journal-title":"Molecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.indcrop.2013.11.039","article-title":"Antioxidant and antidiabetic activity of Thymus quinquecostatus Celak","volume":"52","author":"Hyun","year":"2014","journal-title":"Ind. Crops Prod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jep.2004.10.026","article-title":"A double blind randomised placebo controlled cross over study of a herbal preparation containing Salacia reticulata in the treatment of type 2 diabetes","volume":"97","author":"Jayawardena","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1115646","DOI":"10.1080\/23311932.2015.1115646","article-title":"Effect of extraction solvents on recovery of bioactive compounds and antioxidant properties from macadamia (Macadamia tetraphylla) skin waste","volume":"1","author":"Dailey","year":"2015","journal-title":"Cogent Food Agric."},{"key":"ref_27","first-page":"293","article-title":"Xanthine oxidase inhibitory activity of some Indonesian medicinal plants and active fraction of selected plants","volume":"5","author":"Yumita","year":"2013","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_28","first-page":"16","article-title":"Novel natural anti-gout medication extract from Momordica charantia","volume":"4","author":"Alsultanee","year":"2014","journal-title":"J. Nat. Sci. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"107","DOI":"10.5897\/JPP2015.0349","article-title":"Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using gas chromatography-mass spectrometry (GC-MS)","volume":"7","author":"Hameed","year":"2015","journal-title":"J. Pharmacogn. Phytother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8","DOI":"10.5897\/JPP2015.0364","article-title":"Analysis of bioactive chemical compounds of Nigella sativa using gas chromatography-mass spectrometry","volume":"8","author":"Hadi","year":"2016","journal-title":"J. Pharmacogn. Phytother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejps.2006.04.006","article-title":"Pharmacological properties of the ubiquitous natural product betulin","volume":"29","author":"Alakurtti","year":"2006","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.trstmh.2008.09.008","article-title":"Paromomycin","volume":"103","author":"Davidson","year":"2009","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_33","first-page":"127","article-title":"Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques","volume":"8","author":"Kadhim","year":"2016","journal-title":"Int. J. Pharmacogn. Phytochem. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9421","DOI":"10.15376\/biores.11.4.9421-9437","article-title":"In vitro bioactivity and antimicrobial activity of Picea abies and Larix decidua wood and bark extracts","volume":"11","author":"Salem","year":"2016","journal-title":"BioResources"},{"key":"ref_35","first-page":"529","article-title":"In vitro Antibacterial Activity Assessment of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Lepidium sativum L. on Human Pathogenic Bacteria","volume":"9","author":"Hussein","year":"2016","journal-title":"Int. J. ChemTech Res."},{"key":"ref_36","first-page":"1019","article-title":"Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria","volume":"7","author":"Ayoola","year":"2008","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_37","first-page":"228","article-title":"Phytochemical constituents of some selected medicinal plants","volume":"3","author":"Njoku","year":"2009","journal-title":"Afr. J. Pure Appl. Chem."},{"key":"ref_38","first-page":"159","article-title":"Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout","volume":"19","author":"Azmi","year":"2012","journal-title":"Int. Food Res. J."},{"key":"ref_39","first-page":"90","article-title":"GC-MS analysis of Cassia italica leaf methanol extract","volume":"5","author":"Sermakkani","year":"2012","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"685","DOI":"10.5897\/AJB2005.000-3127","article-title":"Phytochemical constituents of some Nigerian medicinal plants","volume":"4","author":"Edeoga","year":"2005","journal-title":"Afr. J. Biotechnol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/11\/2658\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:36:36Z","timestamp":1760175396000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/11\/2658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,8]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["molecules25112658"],"URL":"https:\/\/doi.org\/10.3390\/molecules25112658","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,8]]}}}