{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T03:05:36Z","timestamp":1783566336422,"version":"3.55.0"},"reference-count":72,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T00:00:00Z","timestamp":1595894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hungarian National Research, Development and Innovation Office","award":["GINOP-2.3.2-15-2016-00040"],"award-info":[{"award-number":["GINOP-2.3.2-15-2016-00040"]}]},{"name":"Hungarian National Research, Development and Innovation Office","award":["OTKA PD 106001"],"award-info":[{"award-number":["OTKA PD 106001"]}]},{"name":"Hungarian National Research, Development and Innovation Office","award":["NVKP_ 16-1-2016-0017"],"award-info":[{"award-number":["NVKP_ 16-1-2016-0017"]}]},{"name":"Hungarian National Research, Development and Innovation Office","award":["Bolyai J\u00e1nos fellowship"],"award-info":[{"award-number":["Bolyai J\u00e1nos fellowship"]}]},{"name":"Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak Academy of Sciences","award":["VEGA no. 2\/0104\/20"],"award-info":[{"award-number":["VEGA no. 2\/0104\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Search for new cardioprotective therapies is of great importance since no cardioprotective drugs are available on the market. In line with this need, several natural biomolecules have been extensively tested for their potential cardioprotective effects. Previously, we have shown that biglycan, a member of a diverse group of small leucine-rich proteoglycans, enhanced the expression of cardioprotective genes and decreased ischemia\/reperfusion-induced cardiomyocyte death via a TLR-4 dependent mechanism. Therefore, in the present study we aimed to test whether decorin, a small leucine-rich proteoglycan closely related to biglycan, could exert cardiocytoprotection and to reveal possible downstream signaling pathways. Methods: Primary cardiomyocytes isolated from neonatal and adult rat hearts were treated with 0 (Vehicle), 1, 3, 10, 30 and 100 nM decorin as 20 h pretreatment and maintained throughout simulated ischemia and reperfusion (SI\/R). In separate experiments, to test the mechanism of decorin-induced cardio protection, 3 nM decorin was applied in combination with inhibitors of known survival pathways, that is, the NOS inhibitor L-NAME, the PKG inhibitor KT-5823 and the TLR-4 inhibitor TAK-242, respectively. mRNA expression changes were measured after SI\/R injury. Results: Cell viability of both neonatal and adult cardiomyocytes was significantly decreased due to SI\/R injury. Decorin at 1, 3 and 10 nM concentrations significantly increased the survival of both neonatal and adult myocytes after SI\/R. At 3nM (the most pronounced protective concentration), it had no effect on apoptotic rate of neonatal cardiac myocytes. No one of the inhibitors of survival pathways (L-NAME, KT-5823, TAK-242) influenced the cardiocytoprotective effect of decorin. MYND-type containing 19 (Zmynd19) and eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1) were significantly upregulated due to the decorin treatment. In conclusion, this is the first demonstration that decorin exerts a direct cardiocytoprotective effect possibly independent of NO-cGMP-PKG and TLR-4 dependent survival signaling.<\/jats:p>","DOI":"10.3390\/molecules25153426","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T12:15:38Z","timestamp":1596111338000},"page":"3426","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Decorin Protects Cardiac Myocytes against Simulated Ischemia\/Reperfusion Injury"],"prefix":"10.3390","volume":"25","author":[{"given":"Ren\u00e1ta","family":"G\u00e1sp\u00e1r","sequence":"first","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Interdisciplinary Excellence Centre, University of Szeged, Dom ter 9, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamilla","family":"G\u00f6m\u00f6ri","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, Dom ter 12, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadett","family":"Kiss","sequence":"additional","affiliation":[{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"},{"name":"MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1089 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1gnes","family":"Sz\u00e1ntai","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, Dom ter 12, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1nos","family":"P\u00e1l\u00f3czi","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, Dom ter 12, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n V.","family":"Varga","sequence":"additional","affiliation":[{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Pipis","sequence":"additional","affiliation":[{"name":"Pharmahungary Group, Hajnoczy utca 6, H-6722 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barnab\u00e1s","family":"V\u00e1radi","sequence":"additional","affiliation":[{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bence","family":"\u00c1gg","sequence":"additional","affiliation":[{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"},{"name":"MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1089 Budapest, Hungary"},{"name":"Pharmahungary Group, Hajnoczy utca 6, H-6722 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Csont","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Interdisciplinary Excellence Centre, University of Szeged, Dom ter 9, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5210-5184","authenticated-orcid":false,"given":"P\u00e9ter","family":"Ferdinandy","sequence":"additional","affiliation":[{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"},{"name":"MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1089 Budapest, Hungary"},{"name":"Pharmahungary Group, Hajnoczy utca 6, H-6722 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Bartekov\u00e1","sequence":"additional","affiliation":[{"name":"Institute for Heart Research, Centre of Experimental Medicine, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 841 04 Bratislava, Slovak"},{"name":"Institute of Physiology, Comenius University in Bratislava, Sasinkova 2, 813 72 Bratislava, Slovak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anik\u00f3","family":"G\u00f6rbe","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, Dom ter 12, H-6720 Szeged, Hungary"},{"name":"Cardiometabolic Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, H-1089 Budapest, Hungary"},{"name":"MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, H-1089 Budapest, Hungary"},{"name":"Pharmahungary Group, Hajnoczy utca 6, H-6722 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/cvr\/cvt004","article-title":"Translating cardioprotection for patient benefit: Position paper from the Working Group of 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