{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:07:06Z","timestamp":1790370426911,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T00:00:00Z","timestamp":1597708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Natural products remain a viable source of novel therapeutics, and as detection and extraction techniques improve, we can identify more molecules from a broader set of plant tissues. The aim of this study was an investigation of the cytotoxic and anti-plasmodial activities of the methanol extract from Stephania dielsiana Y.C. Wu leaves and its isolated compounds. Our study led to the isolation of seven alkaloids, among which oxostephanine (1) is the most active against several cancer cell lines including HeLa, MDA-MB231, MDA-MB-468, MCF-7, and non-cancer cell lines, such as 184B5 and MCF10A, with IC50 values ranging from 1.66 to 4.35 \u03bcM. Morever, oxostephanine (1) is on average two-fold more active against cancer cells than stephanine (3), having a similar chemical structure. Cells treated with oxostephanine (1) are arrested at G2\/M cell cycle, followed by the formation of aneuploidy and apoptotic cell death. The G2\/M arrest appears to be due, at least in part, to the inactivation of Aurora kinases, which is implicated in the onset and progression of many forms of human cancer. An in-silico molecular modeling study suggests that oxostephanine (1) binds to the ATP binding pocket of Aurora kinases to inactivate their activities. Unlike oxostephanine (1), thailandine (2) is highly effective against only the triple-negative MDA-MB-468 breast cancer cells. However, it showed excellent selectivity against the cancer cell line when compared to its effects on non-cancer cells. Furthermore, thailandine (2) showed excellent anti-plasmodial activity against both chloroquine-susceptible 3D7 and chloroquine-resistant W2 Plasmodium falciparum strains. The structure\u2013activity relationship of isolated compound was also discussed in this study. The results of this study support the traditional use of Stephania dielsiana Y.C. Wu and the lead molecules identified can be further optimized for the development of highly effective and safe anti-cancer and anti-plasmodial drugs.<\/jats:p>","DOI":"10.3390\/molecules25163755","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T12:48:43Z","timestamp":1597754923000},"page":"3755","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Cytotoxic and Anti-Plasmodial Activities of Stephania dielsiana Y.C. Wu Extracts and the Isolated Compounds"],"prefix":"10.3390","volume":"25","author":[{"given":"James","family":"Knockleby","sequence":"first","affiliation":[{"name":"Health Science North Research Institute, 56 Walford Road, Sudbury, ON P3E 2H2, Canada"},{"name":"Department of Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2360-3803","authenticated-orcid":false,"given":"Bruno","family":"Pradines","sequence":"additional","affiliation":[{"name":"D\u00e9partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, 13005 Marseille, France"},{"name":"Aix Marseille University, IRD, SSA, AP-HM, VITROME, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"},{"name":"Centre National de R\u00e9f\u00e9rence du Paludisme, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2322-5427","authenticated-orcid":false,"given":"Mathieu","family":"Gendrot","sequence":"additional","affiliation":[{"name":"D\u00e9partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, 13005 Marseille, France"},{"name":"Aix Marseille University, IRD, SSA, AP-HM, VITROME, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Mosnier","sequence":"additional","affiliation":[{"name":"D\u00e9partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, 13005 Marseille, France"},{"name":"Aix Marseille University, IRD, SSA, AP-HM, VITROME, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"},{"name":"Centre National de R\u00e9f\u00e9rence du Paludisme, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh Tam","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Graduate University of Science and Technology and Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 70750, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-6925","authenticated-orcid":false,"given":"Thi Thuy","family":"Trinh","sequence":"additional","affiliation":[{"name":"Graduate University of Science and Technology and Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 70750, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Health Science North Research Institute, 56 Walford Road, Sudbury, ON P3E 2H2, Canada"},{"name":"Departments of Medicine, University of Ottawa Medical School, Ottawa, ON K1H 5M8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6101-5252","authenticated-orcid":false,"given":"Phuong Mai","family":"Le","sequence":"additional","affiliation":[{"name":"National Research Council Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejmech.2017.08.045","article-title":"A comprehensive review on Aurora kinase: Small molecule inhibitors and clinical trial studies","volume":"140","author":"Borisa","year":"2017","journal-title":"Eur. 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