{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:27:28Z","timestamp":1781821648518,"version":"3.54.5"},"reference-count":48,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Research institutes of Standards and Science","award":["KRISS-2020-GP2020-0003"],"award-info":[{"award-number":["KRISS-2020-GP2020-0003"]}]},{"DOI":"10.13039\/501100009338","name":"National Marine Biodiversity Institute of Korea","doi-asserted-by":"publisher","award":["2020M00600"],"award-info":[{"award-number":["2020M00600"]}],"id":[{"id":"10.13039\/501100009338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003668","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["710003-07-7-SB120, 116075-3"],"award-info":[{"award-number":["710003-07-7-SB120, 116075-3"]}],"id":[{"id":"10.13039\/501100003668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003654","name":"Korea Environmental Industry and Technology Institute","doi-asserted-by":"publisher","award":["A117-00197-0703-0"],"award-info":[{"award-number":["A117-00197-0703-0"]}],"id":[{"id":"10.13039\/501100003654","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A potential natural melanogenesis inhibitor was discovered in the form of a sesquiterpene isolated from the flowers of Inula britannica, specifically 6-O-isobutyrylbritannilactone (IBL). We evaluated the antimelanogenesis effects of IBL on B16F10 melanocytes and zebrafish embryos. As a result, we found that 3-isobutyl-1-methylxanthine (IBMX)-induced melanin production was reduced in a dose-dependent manner in B16F10 cells by IBL. We also analyzed B16F10 cells that were and were not treated with IBMX, investigating the melanin concentration, tyrosinase activity, mRNA levels. We also studied the protein expressions of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related proteins (TRP1, and TRP2). Furthermore, we found that melanin synthesis and tyrosinase expression were also inhibited by IBL through the modulation of the following signaling pathways: ERK, phosphoinositide 3-kinase (PI3K)\/AKT, and CREB. In addition, we studied antimelanogenic activity using zebrafish embryos and found that the embryos had significantly reduced pigmentation in the IBL-treated specimens compared to the untreated controls.<\/jats:p>","DOI":"10.3390\/molecules25173887","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T09:56:58Z","timestamp":1598435818000},"page":"3887","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Anti-Melanogenesis Activity of 6-O-Isobutyrylbritannilactone from Inula britannica on B16F10 Melanocytes and In Vivo Zebrafish Models"],"prefix":"10.3390","volume":"25","author":[{"given":"Dae Kil","family":"Jang","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, Dongguk University, Seoul 10326, Korea"},{"name":"StarlingForce Co., Ltd., Seoul 08511, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chau Ha","family":"Pham","sequence":"additional","affiliation":[{"name":"Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Korea"},{"name":"Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ik Soo","family":"Lee","sequence":"additional","affiliation":[{"name":"Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9870-0923","authenticated-orcid":false,"given":"Seung-Hyun","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Applied Marine Bioresource Science, National Marine Biodiversity Institute of Korea, Seocheon, Chungnam 33662, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hye","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Applied Marine Bioresource Science, National Marine Biodiversity Institute of Korea, Seocheon, Chungnam 33662, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Seung","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Dongguk University, Seoul 10326, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5951-2137","authenticated-orcid":false,"given":"Hee Min","family":"Yoo","sequence":"additional","affiliation":[{"name":"Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1242\/dev.106567","article-title":"The melanocyte lineage in development and disease","volume":"142","author":"Mort","year":"2015","journal-title":"Development"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1096\/fj.06-6649rev","article-title":"Human skin pigmentation: Melanocytes modulate skin color in response to stress","volume":"21","author":"Costin","year":"2007","journal-title":"FASEB J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1007\/s00018-009-8703-8","article-title":"Cellular mechanisms regulating human melanogenesis","volume":"66","author":"Park","year":"2009","journal-title":"Cell. 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