{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:57:36Z","timestamp":1787029056210,"version":"build-2736575974"},"reference-count":15,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In research on natural molecules with cytotoxic activity that can be used for the development of new anticancer agents, the cytotoxic activity of hexane, chloroform, and methanol extracts from the roots of Acacia schaffneri against colon, lung, and skin cancer cell lines was explored. The hexane extract showed the best activity with an average IC50 of 10.6 \u00b5g mL\u22121. From this extract, three diterpenoids, phyllocladan-16\u03b1,19-diol (1), phyllocladan-16\u03b1-ol (2), and phylloclad-16-en-3-ol (3), were isolated and characterized by their physical and spectroscopic properties. Diterpenoids 1 and 2 were tested against the same cancer cell lines, as well as their healthy counterparts, CCD841 CoN, MRC5, and VH10, respectively. Compound 1 showed moderate activity (IC50 values between 24 and 70 \u03bcg mL\u22121), although it showed a selective effect against cancer cell lines. Compound 2 was practically inactive. The cytotoxicity mechanism of 1 was analyzed by cell cycle, indicating that the compound induces G0\/G1 cell cycle arrest. This effect might be generated by DNA alkylation damage. In addition, compound 1 decreased migration of HT29 cells.<\/jats:p>","DOI":"10.3390\/molecules25173944","type":"journal-article","created":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T06:06:22Z","timestamp":1598767582000},"page":"3944","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Isolation and Cytotoxic Activity of Phyllocladanes from the Roots of Acacia schaffneri (Leguminosae)"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0144-3009","authenticated-orcid":false,"given":"Jos\u00e9 de Jes\u00fas","family":"Manr\u00edquez-Torres","sequence":"first","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1954-2857","authenticated-orcid":false,"given":"Marco Antonio","family":"Hern\u00e1ndez-Lepe","sequence":"additional","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-7601","authenticated-orcid":false,"given":"Jos\u00e9 Rom\u00e1n","family":"Ch\u00e1vez-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Gonz\u00e1lez-Reyes","sequence":"additional","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idanya Rub\u00ed","family":"Seraf\u00edn-Higuera","sequence":"additional","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3801-4075","authenticated-orcid":false,"given":"Genaro","family":"Rodr\u00edguez-Uribe","sequence":"additional","affiliation":[{"name":"Medical and Psychology School, Autonomous University of Baja California, Universidad 14418, Parque Internacional Industrial Tijuana, Tijuana 22390, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas Mart\u00edn","family":"Torres-Valencia","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Autonomous University of Hidalgo, Km 4.5 Carretera Pachuca-Tulancingo, Mineral de la Reforma 42184, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21590","article-title":"Cancer Statistics, 2020","volume":"70","author":"Siegel","year":"2020","journal-title":"CA Cancer J. 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