{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T06:34:10Z","timestamp":1780814050498,"version":"3.54.1"},"reference-count":43,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Leading Research Groups support project from the subsidy increased for the period 2020\u20132025 in the amount of 2% of the subsidy referred to in Art. 387 (3) of the Law of 20 July 2018 on Higher Education and Science, obtained in 2019.","award":["the Leading Research Groups support project from the subsidy increased for the period 2020\u20132025 in the amount of 2% of the subsidy referred to in Art. 387 (3) of the Law of 20 July 2018 on Higher Education and Science, obtained in 2019."],"award-info":[{"award-number":["the Leading Research Groups support project from the subsidy increased for the period 2020\u20132025 in the amount of 2% of the subsidy referred to in Art. 387 (3) of the Law of 20 July 2018 on Higher Education and Science, obtained in 2019."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chalcones are interesting candidates for anti-cancer drugs due to the ease of their synthesis and their extensive biological activity. The study presents antitumor activity of newly synthesized chalcone analogues with a methoxy group on a panel of canine lymphoma and leukemia cell lines. The antiproliferative effect of the 2\u2032-hydroxychalcone and its methoxylated derivatives was evaluated in MTT assay after 48 h of treatment in different concentrations. The proapoptotic activity was studied by cytometric analysis of cells stained with Annexin V\/FITC and propidium iodide and by measure caspases 3\/7 and 8 activation. The DNA damage was evaluated by Western blot analysis of phosphorylated histone H2AX. The new compounds had selective antiproliferative activity against the studied cell lines, the most effective were the 2\u2032-hydroxy-2\u2033,5\u2033-dimethoxychalcone and 2\u2032-hydroxy-4\u2032,6\u2032-dimethoxychalcone. 2\u2032-Hydroxychalcone and the two most active derivatives induced apoptosis and caspases participation, but some percentage of necrotic cells was also observed. Comparing phosphatidylserine externalization after treatment with the different compounds it was noted that the addition of two methoxy groups increased the proapoptotic potential. The most active compounds triggered DNA damage even in the cell lines resistant to chalcone-induced apoptosis. The results confirmed that the analogues could have anticancer potential in the treatment of canine lymphoma or leukemia.<\/jats:p>","DOI":"10.3390\/molecules25194362","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T03:03:39Z","timestamp":1600916619000},"page":"4362","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Chalcone Methoxy Derivatives Exhibit Antiproliferative and Proapoptotic Activity on Canine Lymphoma and Leukemia Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7449-1661","authenticated-orcid":false,"given":"Aleksandra","family":"Pawlak","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wroc\u0142aw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9725-7969","authenticated-orcid":false,"given":"Marta","family":"Henklewska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wroc\u0142aw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5377-6609","authenticated-orcid":false,"given":"Beatriz","family":"Hern\u00e1ndez Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wroc\u0142aw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0646-2295","authenticated-orcid":false,"given":"Mateusz","family":"\u0141u\u017cny","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-8608","authenticated-orcid":false,"given":"Ewa","family":"Koz\u0142owska","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u017cena","family":"Obmi\u0144ska-Mrukowicz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wroc\u0142aw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9757-6684","authenticated-orcid":false,"given":"Tomasz","family":"Janeczko","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"30379","DOI":"10.1039\/C8RA04669G","article-title":"Microbial transformations of 4\u2032-methylchalcones as an efficient method of obtaining novel alcohol and dihydrochalcone derivatives with antimicrobial activity","volume":"8","author":"Potaniec","year":"2018","journal-title":"RSC Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.ejmech.2015.01.051","article-title":"Chalcones and their therapeutic targets for the management of diabetes: Structural and pharmacological perspectives","volume":"92","author":"Mahapatra","year":"2015","journal-title":"Eur. 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