{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:07:09Z","timestamp":1790269629791,"version":"4.1.0"},"reference-count":22,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Sailing Program","award":["20YF1421700"],"award-info":[{"award-number":["20YF1421700"]}]},{"name":"China Postdoctoral Science Foundation funded project","award":["2018M642028"],"award-info":[{"award-number":["2018M642028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Gardenia jasminoides Ellis is a famous fragrant flower in China. Previous pharmacological research mainly focuses on its fruit. In this study, the essential oil of the flower of \u2018Shanzhizi\u2019, which was a major variety for traditional Chinese medicine use, was extracted by hydro distillation and analyzed by GC-MS. Mouse anxiety models included open field, elevated plus maze (EPM), and light and dark box (LDB), which were used to evaluate its anxiolytic effect via inhalation. The involvement of monoamine system was studied by pretreatment with neurotransmitter receptor antagonists WAY100635, flumazenil and sulpiride. The monoamine neurotransmitters contents in the prefrontal cortex (PFC) and hippocampus after aroma inhalation were also analyzed. The results showed that inhalation of G. jasminoides essential oil could significantly elevated the time and entries into open arms in EPM tests and the time explored in the light chamber in LDB tests with no sedative effect. WAY100635 and sulpiride, but not flumazenil, blocked its anxiolytic effect. Inhalation of G. jasminoides essential oil significantly down-regulated the 5-HIAA\/5-HT in the PFC and reduced the 5-HIAA content in hippocampus compared to the control treatment. In conclusion, inhalation of gardenia essential oil showed an anxiolytic effect in mice. Monoamine, especially the serotonergic system, was involved in its anxiolytic effect.<\/jats:p>","DOI":"10.3390\/molecules25204702","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T08:14:50Z","timestamp":1602922490000},"page":"4702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Chemical Composition of Essential Oil from Flower of \u2018Shanzhizi\u2019 (Gardenia jasminoides Ellis) and Involvement of Serotonergic System in Its Anxiolytic Effect"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3366-3754","authenticated-orcid":false,"given":"Nan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1021\/acs.jafc.6b03866","article-title":"Discovery and LC-MS characterization of new crocins in Gardeniae fructus and their neuroprotective potential","volume":"65","author":"Ni","year":"2017","journal-title":"J. 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