{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:56:37Z","timestamp":1785430597830,"version":"3.56.0"},"reference-count":59,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:00:00Z","timestamp":1602720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Byttneria pilosa is locally known as Harijora, and used by the native hill-tract people of Bangladesh for the treatment of rheumatalgia, snake bite, syphilis, fractured bones, elephantiasis and an antidote for poisoning. The present study was carried out to determine the possible anti-inflammatory, analgesic, neuropharmacological and anti-diarrhoeal activity of the methanol extract of B. pilosa leaves (MEBPL) through in vitro, in vivo and in silico approaches. In the anti-inflammatory study, evaluated by membrane stabilizing and protein denaturation methods, MEBPL showed a significant and dose dependent inhibition. The analgesic effect of MEBPL tested by inducing acetic acid and formalin revealed significant inhibition of pain in both tests. During the anxiolytic evaluation, the extract exhibited a significant and dose-dependent reduction of anxiety-like behaviour in mice. Similarly, mice treated with MEBPL demonstrated dose-dependent reduction in locomotion effect in the open field test and increased sedative effect in the thiopental sodium induced sleeping test. MEBPL also showed good anti-diarrheal activity in both castor oil induced diarrheal and intestinal motility tests. Besides, a previously isolated compound (beta-sitosterol) exhibited good binding affinity in docking and drug-likeliness properties in ADME\/T studies. Overall, B. pilosa is a biologically active plant and could be a potential source of drug leads, which warrants further advanced study.<\/jats:p>","DOI":"10.3390\/molecules25204737","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T08:14:50Z","timestamp":1602922490000},"page":"4737","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches"],"prefix":"10.3390","volume":"25","author":[{"given":"Mifta Ahmed","family":"Jyoti","sequence":"first","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niloy","family":"Barua","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Shafaet","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"},{"name":"Department of Theoretical and Computational Chemistry, University of Dhaka, Dhaka 1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muminul","family":"Hoque","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahmina Akter","family":"Bristy","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabnur","family":"Mahmud","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2329-0867","authenticated-orcid":false,"given":"Kamruzzaman","family":"Kamruzzaman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1330-2169","authenticated-orcid":false,"given":"Md.","family":"Adnan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"},{"name":"Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-8662","authenticated-orcid":false,"given":"Md. Nazim Uddin","family":"Chy","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2767-2646","authenticated-orcid":false,"given":"Arkajyoti","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mir Ezharul","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-2272","authenticated-orcid":false,"given":"Talha Bin","family":"Emran","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9215-9737","authenticated-orcid":false,"given":"Jesus","family":"Simal-Gandara","sequence":"additional","affiliation":[{"name":"Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo\u2014Ourense Campus, E32004 Ourense, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"190","DOI":"10.4103\/0973-1296.157734","article-title":"The potential of selected Australian medicinal plants with anti-Proteus activity for the treatment and prevention of rheumatoid arthritis","volume":"11","author":"Cock","year":"2015","journal-title":"Pharmacogn. Mag."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Amat, N., Amat, R., Abdureyim, S., Hoxur, P., Osman, Z., Mamut, D., and Kijjoa, A. (2014). Aqueous extract of Dioscorea opposita thunb. normalizes the hypertension in 2K1C hypertensive rats. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-36"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.jpain.2016.01.337","article-title":"(530) Distress intolerance and prescription opioid misuse among patients with chronic pain","volume":"17","author":"Cornelius","year":"2016","journal-title":"J. Pain"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.jep.2019.01.025","article-title":"Evaluation of anti-nociceptive and anti-inflammatory activities of the methanol extract of Holigarna caustica (Dennst.) Oken leaves","volume":"236","author":"Adnan","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Adnan, M., Chy, N.U., Kamal, A.M., Chowdhury, R., Islam, S., Hossain, A., Tareq, A.M., Bhuiyan, I.H., Uddin, N., and Tahamina, A. (2020). Unveiling pharmacological responses and potential targets insights of identified bioactive constituents of Cuscuta reflexa Roxb. leaves through in vivo and in silico approaches. Pharmaceuticals, 13.","DOI":"10.3390\/ph13030050"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4306\/pi.2015.12.1.1","article-title":"Pain and depression: A neurobiological perspective of their relationship","volume":"12","author":"Han","year":"2015","journal-title":"Psychiatry Investig."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hasanat, A., Kabir, M.S.H., Ansari, A., Chowdhury, T.A., Hossain, M.M., Islam, M.N., Ahmed, S., Chy, N.U., and Kamal, A.M. (2019). Ficus cunia Buch.-Ham. ex Roxb.(leaves): An experimental evaluation of the cytotoxicity, thrombolytic, analgesic and neuropharmacological activities of its methanol extract. J. Basic Clin. Physiol. Pharmacol., 30.","DOI":"10.1515\/jbcpp-2016-0140"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pollmann, A.S., Murphy, A.L., Bergman, J.C., and Gardner, D.M. (2015). Deprescribing benzodiazepines and Z-drugs in community-dwelling adults: A scoping review. BMC Pharmacol. Toxicol., 16.","DOI":"10.1186\/s40360-015-0019-8"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"116","DOI":"10.5152\/eurasianjmed.2018.0010","article-title":"Effects of Nonsteroidal Anti-Inflammatory Drugs at the Molecular Level","volume":"50","author":"Gunaydin","year":"2018","journal-title":"Eurasian J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/S0016-5085(71)80003-3","article-title":"Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum","volume":"60","author":"Pierce","year":"1971","journal-title":"Gastroenterology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.3390\/molecules24071367","article-title":"Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D. Don) & Mondal leaves through in vivo, in vitro, and in silico approaches","volume":"24","author":"Adnan","year":"2019","journal-title":"Molecules"},{"key":"ref_12","unstructured":"Khine, K.K. (2019). Phytochemical screening and some bioactivities of Byttneria pilosa Roxb. (Sat-le-pyat) Leaves and Stems. 2nd Myanmar Korea Conference Research Journal, Dagon University. Available online: https:\/\/www.dagonuniversity.edu.mm\/research\/."},{"key":"ref_13","first-page":"35","article-title":"Evaluation of thrombolytic and cytotoxic activities of an ornamental medicinal plant: Byttneria pilosa","volume":"3","author":"Ibrahim","year":"2015","journal-title":"Am. J. Biomed. Res."},{"key":"ref_14","first-page":"1585","article-title":"Possible anti-obesity activity of methanol extract of Byttneria pilosa Roxb. leaves","volume":"23","author":"Zaman","year":"2015","journal-title":"Middle-East. J. Sci. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Rahman, A., Sultana, R., Bin Emran, T., Islam, M.S., Chakma, J.S., Rashid, H., Hasan, C.M.M., and Rahman, M.A. (2013). Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement. Altern. Med., 13.","DOI":"10.1186\/1472-6882-13-25"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rashid, U., Khan, M.R., Jan, S., Bokhari, J., and Shah, N.A. (2013). Assessment of phytochemicals, antimicrobial and cytotoxic activities of extract and fractions from Fagonia olivieri (Zygophyllaceae). BMC Complement. Altern. Med., 13.","DOI":"10.1186\/1472-6882-13-167"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Williamson, E.M. (2017). Herbal Neurotoxicity: An Introduction to Its Occurrence and Causes in Toxicology of Herbal Products, Springer.","DOI":"10.1007\/978-3-319-43806-1_14"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.sajb.2017.07.003","article-title":"Anti-inflammatory, analgesic and antioxidant effects of phenolic compound from Algerian Mentha rotundifolia L. leaves on experimental animals","volume":"113","author":"Boussouf","year":"2017","journal-title":"S. Afr. J. Bot."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4229","DOI":"10.1039\/C7FO00917H","article-title":"Phenolic compounds, antioxidant activity, antiproliferative activity and bioaccessibility of Sea buckthorn (Hippopha\u00eb rhamnoides L.) berries as affected byin vitrodigestion","volume":"8","author":"Guo","year":"2017","journal-title":"Food Funct."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1517\/13543776.2014.870154","article-title":"Terpenes and derivatives as a new perspective for pain treatment: A patent review","volume":"24","author":"Serafini","year":"2014","journal-title":"Expert Opin. Ther. Patents"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.ijantimicag.2005.09.002","article-title":"Antimicrobial activity of flavonoids","volume":"26","author":"Cushnie","year":"2005","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/2008-2231-20-76","article-title":"Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum)","volume":"20","author":"Anosike","year":"2012","journal-title":"DARU J. Pharm. Sci."},{"key":"ref_23","first-page":"1","article-title":"Inhibition of protein denaturation by antirheumatic or antiphlogistic agents","volume":"149","author":"Mizushima","year":"1964","journal-title":"Arch. Int. Pharmacodyn.Ther."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gillette, J.R., Heinzelman, R.V., Szmuszkovicz, J., Leemann, H.G., Stich, K., Thomas, M., Martin-Smith, M., Khatoon, T., and Kunz, W. (1963). Progress in Drug Research\/Fortschritte der Arzneimittelforschung\/Progr\u00e8s des recherches pharmaceutiques, Springer Science and Business Media LLC.","DOI":"10.1007\/978-3-0348-7050-4"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3923\/ijp.2010.63.67","article-title":"Analgesic Activity of the Different Fractions of the Aerial Parts of Commelina benghalensis Linn","volume":"6","author":"Hasan","year":"2010","journal-title":"Int. J. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s11418-007-0224-x","article-title":"Antinociceptive, anti-inflammatory, and antipyretic properties of an aqueous extract of Dicranopteris linearis leaves in experimental animal models","volume":"62","author":"Zakaria","year":"2008","journal-title":"J. Nat. Med."},{"key":"ref_27","first-page":"100772","article-title":"In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)","volume":"23","author":"Barua","year":"2020","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_28","first-page":"215","article-title":"Preliminary pharmacological activity of the methanolic extract of Premna integrifolia barks in rats","volume":"4","author":"Khatun","year":"2014","journal-title":"Avicenna J. Phytomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"92","DOI":"10.4314\/ajtcam.v5i1.31261","article-title":"Phytochemical, Analgesic and Anti-Inflammatory Effects Of The Ethylacetate Extract Of The Leaves Of Pseudocedrella kotschyii","volume":"5","author":"Musa","year":"2008","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1248\/bpb.29.693","article-title":"Immunomodulatory and Anti-inflammatory Effects of Semecarpus anacardium LINN. Nut Milk Extract in Experimental Inflammatory Conditions","volume":"29","author":"Ramprasath","year":"2006","journal-title":"Biol. Pharm. Bull."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1096\/fasebj.4.5.2155149","article-title":"Molecular biology of GABA A receptors","volume":"4","author":"Olsen","year":"1990","journal-title":"FASEB J."},{"key":"ref_32","first-page":"165","article-title":"GABA and Glutamate Transporters in Brain","volume":"4","author":"Ezhou","year":"2013","journal-title":"Front. Endocrinol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e04895","DOI":"10.1016\/j.heliyon.2020.e04895","article-title":"An integrated exploration of pharmacological potencies of Bischofia javanica (Blume) leaves through experimental and computational modeling","volume":"6","author":"Chowdhury","year":"2020","journal-title":"Heliyon"},{"key":"ref_34","first-page":"20","article-title":"Studies on the anti-diarrheal properties of seed extract of Monodora tenuifolia","volume":"2","author":"Ezenwali","year":"2010","journal-title":"Int. J. App. Res. Nat. Prod."},{"key":"ref_35","first-page":"313","article-title":"Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega","volume":"3","author":"Raju","year":"2013","journal-title":"Avicenna J. Phytomed."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.2174\/157340911795677602","article-title":"Molecular Docking: A Powerful Approach for Structure-Based Drug Discovery","volume":"7","author":"Meng","year":"2011","journal-title":"Curr. Comput. Drug Des."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0304-3959(83)90201-4","article-title":"Ethical guidelines for investigations of experimental pain in conscious animals","volume":"16","author":"Zimmermann","year":"1983","journal-title":"Pain"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1055\/s-0029-1185950","article-title":"Antinociceptive Activity of the Essential Oil of Zingiber zerumbet","volume":"72","author":"Sulaiman","year":"2010","journal-title":"Planta Medica"},{"key":"ref_40","unstructured":"Harborne, A. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Springer Science & Business Media."},{"key":"ref_41","first-page":"3129","article-title":"Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata","volume":"7","author":"Omale","year":"2008","journal-title":"Afr. J. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1515\/jbcpp-2016-0023","article-title":"Anti-inflammatory, anti-diarrheal, thrombolytic and cytotoxic activities of an ornamental medicinal plant: Persicaria orientalis","volume":"28","author":"Ansari","year":"2017","journal-title":"J. Basic Clin. Physiol. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jep.2003.12.030","article-title":"Anti-inflammatory and analgesic activity of Peperomia pellucida (L.) HBK (Piperaceae)","volume":"91","author":"Dmitrieva","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_44","first-page":"385","article-title":"Antiinflammatory, analgesic and antipyretic activities of ethanolic root extract of Croton zambesicus","volume":"23","author":"Okokon","year":"2010","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0944-7113(96)80027-4","article-title":"Effects of Hypericum perforatum L. and Hypericum calycinum L. extracts on the central nervous system in mice","volume":"3","author":"Aydin","year":"1996","journal-title":"Phytomedicine"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0021-5198(19)36218-3","article-title":"A psychopharmacological analysis of behavior in rats","volume":"21","author":"Gupta","year":"1971","journal-title":"Jpn. J. Pharmacol."},{"key":"ref_47","first-page":"2029","article-title":"Neuro-pharmacological studies on SB 5833, a new psychotherapeutic agent of the benzodiazepine class","volume":"24","author":"Ferrini","year":"1974","journal-title":"Arzneimittelforschung"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0378-8741(00)00379-2","article-title":"Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea","volume":"76","author":"Shoba","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"ref_49","first-page":"291","article-title":"Nitric oxide and castor oil-induced diarrhea","volume":"268","author":"Mascolo","year":"1994","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10822-013-9644-8","article-title":"Protein and ligand preparation: Parameters, protocols, and influence on virtual screening enrichments","volume":"27","author":"Sastry","year":"2013","journal-title":"J. Comput. Mol. Des."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Jahan, I., Tona, M.R., Sharmin, S., Sayeed, M.A., Tania, F.Z., Paul, A., Chy, M.N.U., Rakib, A., Emran, T.B., and Simal-Gandara, J. (2020). GC-MS Phytochemical Profiling, Pharmacological Properties, and In Silico Studies of Chukrasia velutina Leaves: A Novel Source for Bioactive Agents. Molecules, 25.","DOI":"10.3390\/molecules25153536"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"28096","DOI":"10.1074\/jbc.M701335200","article-title":"Structural Basis of Enantioselective Inhibition of Cyclooxygenase-1 by S-\u03b1-Substituted Indomethacin Ethanolamides","volume":"282","author":"Harman","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_53","first-page":"644","article-title":"Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents","volume":"384","author":"Kurumbail","year":"1996","journal-title":"Nat. Cell Biol."},{"key":"ref_54","first-page":"334","article-title":"X-ray structures and mechanism of the human serotonin transporter","volume":"532","author":"Coleman","year":"2016","journal-title":"Nat. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5365","DOI":"10.1021\/bi500525s","article-title":"Structures of kcsa in complex with symmetrical quaternary ammonium compounds reveal a hydrophobic binding site","volume":"53","author":"Lenaeus","year":"2014","journal-title":"Biochemistry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5893","DOI":"10.1021\/jm500378a","article-title":"Discovery and Early Clinical Development of 2-{6-[2-(3,5-Dichloro-4-pyridyl)acetyl]-2,3-dimethoxyphenoxy}-N-propylacetamide (LEO 29102), a Soft-Drug Inhibitor of Phosphodiesterase 4 for Topical Treatment of Atopic Dermatitis","volume":"57","author":"Felding","year":"2014","journal-title":"J. Med. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1016\/j.bmc.2011.10.080","article-title":"Benzodiazepines and benzotriazepines as protein interaction inhibitors targeting bromodomains of the BET family","volume":"20","author":"Filippakopoulos","year":"2012","journal-title":"Bioorg. Med. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"18537","DOI":"10.1073\/pnas.0808082105","article-title":"Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin","volume":"105","author":"Kudryashov","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Natarajan, A., Sugumar, S., Bitragunta, S., and Balasubramanyan, N. (2015). Molecular docking studies of (4Z, 12Z)-cyclopentadeca-4, 12-dienone from Grewia hirsuta with some targets related to type 2 diabetes. BMC Complement. Altern. Med., 15.","DOI":"10.1186\/s12906-015-0588-5"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/20\/4737\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:22:05Z","timestamp":1760178125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/25\/20\/4737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,15]]},"references-count":59,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["molecules25204737"],"URL":"https:\/\/doi.org\/10.3390\/molecules25204737","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,15]]}}}