{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:14:28Z","timestamp":1776226468972,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Science Research Program through the National Research Foundation of Korea (NRF)","award":["2019R1A2C108414011 and 2019R1A2C108414012"],"award-info":[{"award-number":["2019R1A2C108414011 and 2019R1A2C108414012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The efficacy of \u03b1-cubebenoate isolated from Schisandra chinensis has been previously studied in three disease areas, namely inflammation, sepsis, and allergy, and its role in other diseases is still being explored. To identify the novel function of \u03b1-cubebenoate on lipid metabolism and related inflammatory response, alterations in fat accumulation, lipogenesis, lipolysis, and inflammasome activation were measured in 3T3-L1 preadipocytes and primary adipocytes treated with \u03b1-cubebenoate. Lipid accumulation significantly decreased in MDI (3-isobutyl-1-methylxanthine, dexamethasone, and insulin)-stimulated 3T3-L1 adipocytes treated with \u03b1-cubebenoate without any significant cytotoxicity. The mRNA levels of peroxisome proliferator-activated receptor (PPAR)\u03b3 and CCAAT-enhancer binding protein (C\/EBP) \u03b1 for adipogenesis, as well as adipocyte fatty acid binding protein 2 (aP2) and fatty acid synthetase (FAS) for lipogenesis, were reduced after \u03b1-cubebenoate treatment, while cell cycle arrest at G2\/M stage was restored in the same group. \u03b1-cubebenoate treatment induced glycerol release in primary adipocytes and enhanced expression of lipolytic proteins (HSL, perilipin, and ATGL) expression in MDI-stimulated 3T3-L1 adipocytes. Inflammasome activation and downstream cytokines expression were suppressed with \u03b1-cubebenoate treatment, but the expression of insulin receptor signaling factors was remarkably increased by \u03b1-cubebenoate treatment in MDI-stimulated 3T3-L1 adipocytes. These results indicate that \u03b1-cubebenoate may play a novel role as lipogenesis inhibitor, lipolysis stimulator, and inflammasome suppressor in MDI-stimulated 3T3-L1 adipocytes. Our results provide the possibility that \u03b1-cubebenoate can be considered as one of the candidates for obesity management.<\/jats:p>","DOI":"10.3390\/molecules25214995","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T23:06:12Z","timestamp":1604012772000},"page":"4995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Novel Function of \u03b1-Cubebenoate Derived from Schisandra chinensis as Lipogenesis Inhibitor, Lipolysis Stimulator and Inflammasome Suppressor"],"prefix":"10.3390","volume":"25","author":[{"given":"Su Ji","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Ju","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong Eun","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Whan","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Horticultural Bioscience, College of Natural Resources &amp; Life Science, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5144-1725","authenticated-orcid":false,"given":"Dae Youn","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science (BK21 Four Program), College of Natural Resources &amp; Life Science\/Life and Industry Convergence Research Institute\/Laboratory Animal Resources Center, Pusan National University, Miryang 50463, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nowak, A., Zak\u0142os-Szyda, M., B\u0142asiak, J., Nowak, A., Zhang, Z., and Zhang, B. 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