{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:44:30Z","timestamp":1782474270363,"version":"3.54.5"},"reference-count":58,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1200610"],"award-info":[{"award-number":["1200610"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1180059"],"award-info":[{"award-number":["1180059"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012655","name":"Universidad de Antofagasta","doi-asserted-by":"publisher","award":["NEXER ANT1756"],"award-info":[{"award-number":["NEXER ANT1756"]}],"id":[{"id":"10.13039\/501100012655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p &lt; 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10\u22125 M). Pre-incubation of intestinal segments with 100 \u00b5g\/mL MIE significantly reduced (p &lt; 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10\u22125 M; p &lt; 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg\/kg) for 28 days significantly reduced (p &lt; 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.<\/jats:p>","DOI":"10.3390\/molecules25215149","type":"journal-article","created":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T09:04:34Z","timestamp":1604567074000},"page":"5149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0011-7957","authenticated-orcid":false,"given":"Roberto O.","family":"Yba\u00f1ez-Julca","sequence":"first","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Facultad de Farmacia y Bioqu\u00edmica, Universidad Nacional de Trujillo, Trujillo 13011, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7498-2121","authenticated-orcid":false,"given":"Daniel","family":"Asunci\u00f3n-Alvarez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Facultad de Farmacia y Bioqu\u00edmica, Universidad Nacional de Trujillo, Trujillo 13011, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8515-0654","authenticated-orcid":false,"given":"Ivan M.","family":"Quispe-D\u00edaz","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Facultad de Farmacia y Bioqu\u00edmica, Universidad Nacional de Trujillo, Trujillo 13011, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7997-9295","authenticated-orcid":false,"given":"Javier","family":"Palacios","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Aplicada, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique 1110939, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1803-9272","authenticated-orcid":false,"given":"Jorge","family":"B\u00f3rquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales, Departamento de Qu\u00edmica, Universidad de Antofagasta, Antofagasta 1270300, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4552-3123","authenticated-orcid":false,"given":"Mario J.","family":"Simirgiotis","sequence":"additional","affiliation":[{"name":"Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5110566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shagufta","family":"Perveen","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7092-9382","authenticated-orcid":false,"given":"Chukwuemeka R.","family":"Nwokocha","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Faculty of Medical Sciences, The University of the West Indies, Mona Campus, Kingston 7, KGN, Jamaica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5922-8892","authenticated-orcid":false,"given":"Fredi","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Experimental (EPhyL), Instituto Antofagasta (IA), Universidad de Antofagasta, Antofagasta 1270300, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-8582","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Paredes","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Facultad de Ciencias B\u00e1sicas, Universidad de Antofagasta, Antofagasta 1271155, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10341-018-0367-9","article-title":"Trends in Mango Research as Seen through Science Citation Expanded Index of Web of Science","volume":"60","author":"Kolle","year":"2018","journal-title":"Erwerbs-Obstbau"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1177\/1753944715572958","article-title":"Phytochemical and in vitro and in vivo biological investigation on the antihypertensive activity of mango leaves (Mangifera indica L.)","volume":"9","author":"Ronchi","year":"2015","journal-title":"Ther. 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