{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:53:31Z","timestamp":1790189611620,"version":"4.1.0"},"reference-count":26,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T00:00:00Z","timestamp":1604880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Arrabidaea brachypoda is a plant commonly used for the treatment of kidney stones, arthritis and pain in traditional Brazilian medicine. Different in vitro and in vivo activities, ranging from antinociceptive to anti-Trypanosoma cruzi, have been reported for the dichloromethane root extract of Arrabidaea brachypoda (DCMAB) and isolated compounds. This work aimed to assess the in vitro anti-inflammatory activity in arthritic synoviocytes of the DCMAB, the hydroethanolic extract (HEAB) and three dimeric flavonoids isolated from the DCMAB. These compounds, brachydin A (1), B (2) and C (3), were isolated both by medium pressure liquid and high-speed counter current chromatography. Their quantification was performed by mass spectrometry on both DCMAB and HEAB. IL-1\u03b2 activated human fibroblast-like synoviocytes were incubated with both extracts and isolated compounds to determine the levels of pro-inflammatory cytokine IL-6 by enzyme-linked immunosorbent assay (ELISA). DCMAB inhibited 30% of IL-6 release at 25 \u00b5g\/mL, when compared with controls while HEAB was inactive. IC50 values determined for 2 and 3 were 3-fold higher than 1. The DCMAB activity seems to be linked to higher proportions of compounds 2 and 3 in this extract. These observations could thus explain the traditional use of A. brachypoda roots in the treatment of osteoarthritis.<\/jats:p>","DOI":"10.3390\/molecules25215219","type":"journal-article","created":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T19:08:29Z","timestamp":1604948909000},"page":"5219","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["In Vitro Anti-Inflammatory Activity in Arthritic Synoviocytes of A. brachypoda Root Extracts and Its Unusual Dimeric Flavonoids"],"prefix":"10.3390","volume":"25","author":[{"given":"Carlota","family":"Salgado","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3969-2406","authenticated-orcid":false,"given":"Hugo","family":"Morin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1997-8474","authenticated-orcid":false,"given":"Nayara","family":"Coriolano de Aquino","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica, Universidade Federal do Cear\u00e1, 60450-765 Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4472-8453","authenticated-orcid":false,"given":"Laurence","family":"Neff","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3578-1869","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Quintino da Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Naturais, Centro de Ci\u00eancia Exatas e Tecnologia, Departamento de Qu\u00edmica, 65080-805 S\u00e3o Lu\u00eds-MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner","family":"Vilegas","sequence":"additional","affiliation":[{"name":"Experimental Campus of the Paulista Coast, UNESP-S\u00e3o Paulo State University, 11330-900 S\u00e3o Vicente-SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9614-1099","authenticated-orcid":false,"given":"Laurence","family":"Marcourt","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0125-952X","authenticated-orcid":false,"given":"Jean-Luc","family":"Wolfender","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3752-2198","authenticated-orcid":false,"given":"Olivier","family":"Jordan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emerson","family":"Ferreira Queiroz","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6598-9521","authenticated-orcid":false,"given":"Eric","family":"All\u00e9mann","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland"},{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1211 Geneva-4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0305-1978(99)00076-9","article-title":"The distribution of iridoids in Bignoniaceae","volume":"28","author":"Schripsema","year":"2000","journal-title":"Biochem. 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