{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:51:43Z","timestamp":1790635903247,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Age-related macular degeneration (AMD) is a degenerative disease of the retina where the molecular mechanism involves the production of vascular endothelial growth factor (VEGF), a factor of poor prognosis of the progression of the disease. Previous studies have shown that resveratrol, a polyphenol of grapevines, can prevent VEGF secretion induced by stress from retinal cells. Considering the fundamental role played by VEGF in development and progression of AMD, we investigate the potential effect of red wine extract (RWE) on VEGF secretion and its signaling pathway in human retinal cells ARPE-19. To examine the effect of RWE in ARPE-19, a quantitative and qualitative analysis of the RWE was performed by HPLC MS\/MS. We show for the first time that RWE decreased VEGF-A secretion from ARPE-19 cells and its protein expression in concentration-dependent manner. RWE-induced alteration in VEGF-A production is associated with a down of VEGF-receptor 2 (VEGF-R2) protein expression and its phosphorylated intracytoplasmic domain. Subsequently, the activation of kinase pathway is disturbing and RWE prevents the phosphorylation of MEK and ERK 1\/2 in human retinal cells ARPE-19. Finally, this study sheds light on the interest that the use of polyphenolic cocktails could represent in a prevention strategy.<\/jats:p>","DOI":"10.3390\/molecules25235564","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T09:16:49Z","timestamp":1606468609000},"page":"5564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0038-3809","authenticated-orcid":false,"given":"Clarisse","family":"Cornebise","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavie","family":"Courtaut","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Taillandier-Coindard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Valls-Fonayet","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d\u2019Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-8697","authenticated-orcid":false,"given":"Tristan","family":"Richard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d\u2019Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Monchaud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"Institut de Chimie Mol\u00e9culaire (ICMUB), CNRS UMR6302, UBFC, F-21078 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Aires","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Delmas","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team, Bioactive Molecules and Health Research Group, F-21000 Dijon, France"},{"name":"Centre Anticanc\u00e9reux Georges Fran\u00e7ois Leclerc, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1097\/00001648-199803000-00014","article-title":"Alcohol and mortality in middle-aged men from eastern France","volume":"9","author":"Renaud","year":"1998","journal-title":"Epidemiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/S0009-9120(99)00051-X","article-title":"Moderate alcohol consumption: The gentle face of Janus","volume":"32","author":"Goldberg","year":"1999","journal-title":"Clin. 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