{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T07:42:21Z","timestamp":1773819741103,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["CEITEC 2020 (LQ1601)"],"award-info":[{"award-number":["CEITEC 2020 (LQ1601)"]}]},{"name":"IGA TP MENDELU","award":["AF-IGA-2018-tym005"],"award-info":[{"award-number":["AF-IGA-2018-tym005"]}]},{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"publisher","award":["RO0518"],"award-info":[{"award-number":["RO0518"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Respiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system\u2014lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1\u03b2 (IL-1\u03b2) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL-1\u03b2 were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF\/TOF directly on the tissue cryosections. CD163, IL-1\u03b2 and protegrin-4 precursor were all significantly (p &lt; 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs.<\/jats:p>","DOI":"10.3390\/molecules25235723","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T20:09:40Z","timestamp":1607026180000},"page":"5723","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["MALDI MSI Reveals the Spatial Distribution of Protein Markers in Tracheobronchial Lymph Nodes and Lung of Pigs after Respiratory Infection"],"prefix":"10.3390","volume":"25","author":[{"given":"Tomas","family":"Do","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-714X","authenticated-orcid":false,"given":"Roman","family":"Guran","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, 612 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rea","family":"Jarosova","sequence":"additional","affiliation":[{"name":"Department of Morphology, Physiology and Animal Genetics, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Ondrackova","sequence":"additional","affiliation":[{"name":"Department of Immunology, Veterinary Research Institute, 621 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbysek","family":"Sladek","sequence":"additional","affiliation":[{"name":"Department of Morphology, Physiology and Animal Genetics, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5448-7235","authenticated-orcid":false,"given":"Martin","family":"Faldyna","sequence":"additional","affiliation":[{"name":"Department of Immunology, Veterinary Research Institute, 621 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, 612 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Mendel University in Brno, 613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7607-5058","authenticated-orcid":false,"given":"Ondrej","family":"Zitka","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, 613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, 612 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Mendel University in Brno, 613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.mcp.2019.03.007","article-title":"Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay","volume":"45","author":"Li","year":"2019","journal-title":"Mol. 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