{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:06:28Z","timestamp":1761541588555,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study aimed to investigate how prolonged storage of adult retinal pigment epithelial (ARPE-19) cell sheets affects cell metabolism, morphology, viability, and phenotype. ARPE-19 cell sheets were stored at three temperatures (4 \u00b0C, 16 \u00b0C, and 37 \u00b0C) for three weeks. Metabolic status and morphology of the cells were monitored by sampling medium and examining cells by phase-contrast microscopy, respectively, throughout the storage period. Cell viability was analyzed by flow cytometry, and phenotype was determined by epifluorescence microscopy after the storage. Lactate production and glucose consumption increased heavily, while pH dropped considerably, through storage at 37 \u00b0C compared to 4 \u00b0C and 16 \u00b0C. During storage, morphology started to deteriorate first at 4 \u00b0C, then at 37 \u00b0C, and was maintained the longest at 16 \u00b0C. Viability of the cells after three weeks of storage was best preserved at 16 \u00b0C, while cells stored at 4 \u00b0C and 37 \u00b0C had reduced viability. Dedifferentiation indicated by reduced expression of retinal pigment epithelium-specific protein 65 (RPE65), zonula occludens protein 1 (ZO-1), and occludin after three weeks of storage was noticed in all experimental groups compared to control. We conclude that storage temperature affects the metabolic status of ARPE-19 cells and that 16 \u00b0C reduces metabolic activity while protecting viability and morphology.<\/jats:p>","DOI":"10.3390\/molecules25245809","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T22:15:36Z","timestamp":1607638536000},"page":"5809","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Effects of Prolonged Storage on ARPE-19 Cells Stored at Three Different Storage Temperatures"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7174-9225","authenticated-orcid":false,"given":"Rakibul","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rima Maria","family":"Corraya","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3871-9841","authenticated-orcid":false,"given":"Lara","family":"Pasovic","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"},{"name":"Department of Surgery, Akershus University Hospital, 1478 L\u00f8renskog, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2048-225X","authenticated-orcid":false,"given":"Ayyad Zartasht","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Christian D.","family":"Aass","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1900-4605","authenticated-orcid":false,"given":"Jon Roger","family":"Eidet","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"},{"name":"Department of Ophthalmology, Oslo University Hospital, 0450 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tor Paaske","family":"Utheim","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Oslo University Hospital, 0450 Oslo, Norway"},{"name":"Department of Ophthalmology, Oslo University Hospital, 0450 Oslo, Norway"},{"name":"Department of Ophthalmology, Stavanger University Hospital, 4011 Stavanger, Norway"},{"name":"Department of Ophthalmology, S\u00f8rlandet Hospital Arendal, 4838 Arendal, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1034\/j.1600-0854.2001.21202.x","article-title":"The Polarity of the Retinal Pigment Epithelium","volume":"2","author":"Marmorstein","year":"2001","journal-title":"Traffic"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1056\/NEJMra062326","article-title":"Age-related macular degeneration","volume":"355","year":"2006","journal-title":"N. 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