{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T06:50:55Z","timestamp":1781592655042,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T00:00:00Z","timestamp":1607558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["VP29117938605"],"award-info":[{"award-number":["VP29117938605"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>(1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders.<\/jats:p>","DOI":"10.3390\/molecules25245840","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T22:15:36Z","timestamp":1607638536000},"page":"5840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-6428","authenticated-orcid":false,"given":"Hiroko","family":"Nakayama","sequence":"first","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan"},{"name":"Zebrafish Drug Screening Center, Mie University, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanae","family":"Hata","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izumi","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-795X","authenticated-orcid":false,"given":"Liqing","family":"Zang","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan"},{"name":"Zebrafish Drug Screening Center, Mie University, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngil","family":"Kim","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd, Osaka 544-0012, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djongchi","family":"Chu","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd, Osaka 544-0012, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lekh Raj","family":"Juneja","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd, Osaka 544-0012, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-4013","authenticated-orcid":false,"given":"Norihiro","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan"},{"name":"Zebrafish Drug Screening Center, Mie University, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-8262","authenticated-orcid":false,"given":"Yasuhito","family":"Shimada","sequence":"additional","affiliation":[{"name":"Zebrafish Drug Screening Center, Mie University, Tsu 514-8507, Japan"},{"name":"Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan"},{"name":"Department of Bioinformatics, Mie University Advanced Science Research Promotion Center, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.amepre.2012.01.005","article-title":"Successful weight loss among obese U.S. adults","volume":"42","author":"Nicklas","year":"2012","journal-title":"Am. 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