{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:25:28Z","timestamp":1782109528668,"version":"3.54.5"},"reference-count":73,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T00:00:00Z","timestamp":1608249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Les Laboratoires SERVIER","award":["PI M5113G1900N"],"award-info":[{"award-number":["PI M5113G1900N"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds.<\/jats:p>","DOI":"10.3390\/molecules25245992","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T04:12:01Z","timestamp":1608523921000},"page":"5992","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6673-3843","authenticated-orcid":false,"given":"Rhodin C.","family":"Joseph","sequence":"first","affiliation":[{"name":"Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Federal University of Par\u00e1 (UFPA), Espa\u00e7o Inova\u00e7\u00e3o, Av. Perimetral da Ci\u00eancia, Bel\u00e9m, Par\u00e1 66095-630, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus","family":"Silva da Fonseca Diniz","sequence":"additional","affiliation":[{"name":"Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Federal University of Par\u00e1 (UFPA), Espa\u00e7o Inova\u00e7\u00e3o, Av. Perimetral da Ci\u00eancia, Bel\u00e9m, Par\u00e1 66095-630, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane","family":"Magno do Nascimento","sequence":"additional","affiliation":[{"name":"Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Federal University of Par\u00e1 (UFPA), Espa\u00e7o Inova\u00e7\u00e3o, Av. Perimetral da Ci\u00eancia, Bel\u00e9m, Par\u00e1 66095-630, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abra\u00e3o de Jesus","family":"Barbosa Muribeca","sequence":"additional","affiliation":[{"name":"Chemistry Post-Graduation Program, Institute of Exact and Natural Sciences, UFPA, Av. Bernardo Say\u00e3o, Bel\u00e9m, Par\u00e1 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan Carlos","family":"Costa Santiago","sequence":"additional","affiliation":[{"name":"Chemistry Post-Graduation Program, Institute of Exact and Natural Sciences, UFPA, Av. Bernardo Say\u00e3o, Bel\u00e9m, Par\u00e1 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luziane","family":"da Cunha Borges","sequence":"additional","affiliation":[{"name":"Chemistry Post-Graduation Program, Institute of Exact and Natural Sciences, UFPA, Av. 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