{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:26:10Z","timestamp":1792103170500,"version":"4.4.0"},"reference-count":27,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["5540280"],"award-info":[{"award-number":["5540280"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The present study investigated the antidiabetic properties of the extracts and fractions from leaves and stem bark of M. glabra based on dipeptidyl peptidase-4 (DPP-4) and \u03b1-Amylase inhibitory activity assays. The chloroform extract of the leaves was found to be most active towards inhibition of DPP-4 and \u03b1-Amylase with IC50 of 169.40 \u03bcg\/mL and 303.64 \u03bcg\/mL, respectively. Bioassay-guided fractionation of the leaves\u2019 chloroform extract revealed fraction 4 (CF4) as the most active fraction (DPP-4 IC50: 128.35 \u03bcg\/mL; \u03b1-Amylase IC50: 170.19 \u03bcg\/mL). LC-MS\/MS investigation of CF4 led to the identification of trans-decursidinol (1), swermirin (2), methyl 3,4,5-trimethoxycinnamate (3), renifolin (4), 4\u2032,5,6,7-tetramethoxy-flavone (5), isorhamnetin (6), quercetagetin-3,4\u2032-dimethyl ether (7), 5,3\u2032,4\u2032-trihydroxy-6,7-dimethoxy-flavone (8), and 2-methoxy-5-acetoxy-fruranogermacr-1(10)-en-6-one (9) as the major components. The computational study suggested that (8) and (7) were the most potent DPP-4 and \u03b1-Amylase inhibitors based on their lower binding affinities and extensive interactions with critical amino acid residues of the respective enzymes. The binding affinity of (8) with DPP-4 (\u22128.1 kcal\/mol) was comparable to that of sitagliptin (\u22128.6 kcal\/mol) while the binding affinity of (7) with \u03b1-Amylase (\u22128.6 kcal\/mol) was better than acarbose (\u22126.9 kcal\/mol). These findings highlight the phytochemical profile and potential antidiabetic compounds from M. glabra that may work as an alternative treatment for diabetes.<\/jats:p>","DOI":"10.3390\/molecules26010001","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T20:39:29Z","timestamp":1608669569000},"page":"1","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Identification of Dipeptidyl Peptidase-4 and \u03b1-Amylase Inhibitors from Melicope glabra (Blume) T. G. Hartley (Rutaceae) Using Liquid Chromatography Tandem Mass Spectrometry, In Vitro and In Silico Methods"],"prefix":"10.3390","volume":"26","author":[{"given":"Alexandra","family":"Quek","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nur Kartinee","family":"Kassim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"},{"name":"Integrated Chemical BioPhysics Research, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3345-0265","authenticated-orcid":false,"given":"Muhammad Alif Mohammad","family":"Latif","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khozirah","family":"Shaari","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"},{"name":"Natural Medicines &amp; Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5578-3415","authenticated-orcid":false,"given":"Dai Chuan","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei Cee","family":"Lim","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Mahsa University, Bandar Saujana Putra, Jenjarom, Selangor 42610, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1007\/s13205-018-1528-0","article-title":"Role of medicinal plants in the management of diabetes mellitus: A review","volume":"9","author":"Bindu","year":"2018","journal-title":"3 Biotech"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1590\/S0102-695X2010000400007","article-title":"Immunomodulatory and antibacterial activities of extracts from Rutaceae species","volume":"20","author":"Costa","year":"2010","journal-title":"Rev. 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