{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:40:55Z","timestamp":1783960855581,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,9]],"date-time":"2021-01-09T00:00:00Z","timestamp":1610150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers &quot;Digital biodesign and personalized healthcare&quot;","award":["075-15-2020-913"],"award-info":[{"award-number":["075-15-2020-913"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Atomic force microscopy (AFM)-based fishing is a promising method for the detection of low-abundant proteins. This method is based on the capturing of the target proteins from the analyzed solution onto a solid substrate, with subsequent counting of the captured protein molecules on the substrate surface by AFM. Protein adsorption onto the substrate surface represents one of the key factors determining the capturing efficiency. Accordingly, studying the factors influencing the protein adsorbability onto the substrate surface represents an actual direction in biomedical research. Herein, the influence of water motion in a flow-based system on the protein adsorbability and on its enzymatic activity has been studied with an example of horseradish peroxidase (HRP) enzyme by AFM, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and conventional spectrophotometry. In the experiments, HRP solution was incubated in a setup modeling the flow section of a biosensor communication. The measuring cell with the protein solution was placed near a coiled silicone pipe, through which water was pumped. The adsorbability of the protein onto the surface of the mica substrate has been studied by AFM. It has been demonstrated that incubation of the HRP solution near the coiled silicone pipe with flowing water leads to an increase in its adsorbability onto mica. This is accompanied by a change in the enzyme\u2019s secondary structure, as has been revealed by ATR-FTIR. At the same time, its enzymatic activity remains unchanged. The results reported herein can be useful in the development of models describing the influence of liquid flow on the properties of enzymes and other proteins. The latter is particularly important for the development of biosensors for biomedical applications\u2014particularly for serological analysis, which is intended for the early diagnosis of various types of cancer and infectious diseases. Our results should also be taken into account in studies of the effects of protein aggregation on hemodynamics, which plays a key role in human body functioning.<\/jats:p>","DOI":"10.3390\/molecules26020306","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T21:21:45Z","timestamp":1610313705000},"page":"306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase"],"prefix":"10.3390","volume":"26","author":[{"given":"Yuri D.","family":"Ivanov","sequence":"first","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana O.","family":"Pleshakova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan D.","family":"Shumov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey F.","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia A.","family":"Valueva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A.","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria O.","family":"Ershova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5961-7182","authenticated-orcid":false,"given":"Dmitry I.","family":"Larionov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor V.","family":"Repnikov","sequence":"additional","affiliation":[{"name":"Bruker Ltd., Moscow 119017, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina D.","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow 109472, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim Yu.","family":"Tatur","sequence":"additional","affiliation":[{"name":"Foundation of Perspective Technologies and Novations, Moscow 115682, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor N.","family":"Stepanov","sequence":"additional","affiliation":[{"name":"Foundation of Perspective Technologies and Novations, Moscow 115682, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim S.","family":"Ziborov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry, Moscow 119121, Russia"},{"name":"Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow 125412, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/nbt.1641","article-title":"Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations","volume":"28","author":"Rissin","year":"2010","journal-title":"Nat. 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