{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:55:13Z","timestamp":1780527313102,"version":"3.54.1"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Managing Director of the Educational Fund Management Agency of the Ministry of Finance of the Republic of Indonesia who has provided funding for Productive Innovative Research (RISPRO)","award":["PRJ-52 \/ LPDP \/ 2019"],"award-info":[{"award-number":["PRJ-52 \/ LPDP \/ 2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Piper betle var. nigra is a tropical plant closely related to the common piper. P. betle has also been dubbed a promising source of natural antioxidants in herbal health products, antibacterial, antifungal, antimalarial, cytotoxic activity against the cancer cell lines K562 and HL-60, and antileishmanial. The aim of this study to observation Antimicrobial activity and isolation of chemical compound. The antimicrobial activity of P. betle extract was performed by well diffusion method against two oral pathogenic bacteria (Streptococcus mutans and Streptococcus sanguinis) and opportunistic pathogenic yeast (Candida albicans). The inoculum (bacterial and yeast suspension) was prepared from a 24-h culture on NB for bacterial suspension and on TSB for yeast suspension. Extraction and isolation using various method of chromatography. Isolated compounds were characterized by spectroscopic means. Our study showed antimicrobial activity from crude ethanol extract of leaves P. betle L. var. nigra against two oral pathogenic bacteria and opportunistic pathogenic yeast with concentration 0.5% and 1%. The first report of two new amides derivatives, piperenamide A (1) and piperenamide B (2) in P. betle L. var. nigra.<\/jats:p>","DOI":"10.3390\/molecules26020335","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T11:36:11Z","timestamp":1610364971000},"page":"335","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Novel Amides Derivative with Antimicrobial Activity of Piper betle var. nigra Leaves from Indonesia"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1020-0423","authenticated-orcid":false,"given":"Fajar","family":"Prasetya","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriatno","family":"Salam","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor, Sumedang 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agung","family":"Rahmadani","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"},{"name":"Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor, Sumedang 45363, Indonesia"},{"name":"Departement of Chemistry Education, Faculty of Teaching and Education, Mulawarman University, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kansy","family":"Haikal","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor, Sumedang 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizma","family":"Febrina","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hady","family":"Anshory","sequence":"additional","affiliation":[{"name":"Departement of Pharmacy, Faculty of Mathematics and Natural Sciences, Islamic University of Indonesia, Jogjakarta 55584, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Arifuddin","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vita Olivia","family":"Siregar","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angga Cipta","family":"Narsa","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Herman","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7429-9215","authenticated-orcid":false,"given":"Islamudin","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niken","family":"Indriyanti","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arsyik","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolan","family":"Rusli","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laode","family":"Rijai","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3406-522X","authenticated-orcid":false,"given":"Hadi","family":"Kuncoro","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Kalimantan Timur, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Salehi, B., Zakaria, Z.A., Gyawali, R., Ibrahim, S.A., Rajkovic, J., Shinwari, Z.K., Khan, T., Sharifi-Rad, J., Ozleyen, A., and Turkdonmez, E. 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