{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:18:03Z","timestamp":1762157883900,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"inistry of Science and Technology, Taiwan","award":["MOST 108\u20132320-B-003\u2013003-MY3"],"award-info":[{"award-number":["MOST 108\u20132320-B-003\u2013003-MY3"]}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST-108\u20132320-B-264\u2013001 MY-2"],"award-info":[{"award-number":["MOST-108\u20132320-B-264\u2013001 MY-2"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cutibacterium acnes (formerly Propionibacterium acnes) is one of the major bacterial species responsible for acne vulgaris. Numerous bioactive compounds from Momordica charantia Linn. var. abbreviata Ser. have been isolated and examined for many years. In this study, we evaluated the suppressive effect of two cucurbitane-type triterpenoids, 5\u03b2,19-epoxycucurbita-6,23-dien-3\u03b2,19,25-triol (Kuguacin R; KR) and 3\u03b2,7\u03b2,25-trihydroxycucurbita-5,23-dien-19-al (TCD) on live C. acnes-stimulated in vitro and in vivo inflammatory responses. Using human THP-1 monocytes, KR or TCD suppressed C. acnes-induced production of interleukin (IL)-1\u03b2, IL-6 and IL-8 at least above 56% or 45%, as well as gene expression of these three pro-inflammatory cytokines. However, a significantly strong inhibitory effect on production and expression of tumor necrosis factor (TNF)-\u03b1 was not observed. Both cucurbitanes inhibited C. acnes-induced activation of the myeloid differentiation primary response 88 (MyD88) (up to 62%) and mitogen-activated protein kinases (MAPK) (at least 36%). Furthermore, TCD suppressed the expression of pro-caspase-1 and cleaved caspase-1 (p10). In a separate study, KR or TCD decreased C. acnes-stimulated mouse ear edema by ear thickness (20% or 14%), and reduced IL-1\u03b2-expressing leukocytes and neutrophils in mouse ears. We demonstrated that KR and TCD are potential anti-inflammatory agents for modulating C. acnes-induced inflammation in vitro and in vivo.<\/jats:p>","DOI":"10.3390\/molecules26030579","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T11:13:53Z","timestamp":1611314033000},"page":"579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Suppressive Effect of Two Cucurbitane-Type Triterpenoids from Momordica charantia on Cutibacterium acnes-Induced Inflammatory Responses in Human THP-1 Monocytic Cell and Mouse Models"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0318-0030","authenticated-orcid":false,"given":"Lu-Te","family":"Chuang","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6918-3322","authenticated-orcid":false,"given":"Wen-Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Chen","family":"Hou","sequence":"additional","affiliation":[{"name":"Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Ho","family":"Chyuan","sequence":"additional","affiliation":[{"name":"Hualien District Agricultural Research and Extension Station, Hualien 973, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiang","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi-I","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsung-Hsien","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Taipei Municipal Wan Fang Hospital and Taipei Medical University, Taipei 116, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-0685","authenticated-orcid":false,"given":"Po-Jung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, Taiwan"},{"name":"Program of Nutrition Science, School of Life Science, National Taiwan Normal University, Taipei 116, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"key":"ref_1","first-page":"27","article-title":"The role of inflammation in the pathology of acne","volume":"6","author":"Tanghetti","year":"2013","journal-title":"J. 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